PMID- 34268255
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210717
IS  - 2251-9637 (Print)
IS  - 2251-9645 (Electronic)
IS  - 2251-9637 (Linking)
VI  - 10
IP  - 1
DP  - 2021 Winter
TI  - Level of miR-101a and miR-107 in Human Adipose Mesenchymal Stem Cells Committed 
      to Insulin-producing Cells.
PG  - 68-74
LID - 10.22088/IJMCM.BUMS.10.1.68 [doi]
AB  - Mesenchymal stem cells have the fundamental ability to differentiate into 
      multiple cells such as osteoblasts, neural cells, and insulin-producing cells. 
      MicroRNAs (miRNAs) are single-strand and small non-coding RNAs involved in stem 
      cells orientation into mature cells. There is no comprehensive data about the 
      dynamic of distinct miRNAs during the differentiation of mesenchymal cells from 
      adipose tissue into insulin-producing cells. In this study, we first 
      differentiated adipose-derived mesenchymal stem cells into insulin-producing 
      cells by a three-stepwise protocol. Differentiation capacity was confirmed by the 
      dithizone staining method and hormone (insulin and C peptide) release analysis 
      via electrochemiluminescence technique. In the final phase, the expression of 
      hsa-miR-101a and hsa-miR-107 and two pancreatic genes, sex-determining region 
      Y-box (SOX) 6 and neuronal differentiation 1 (NeuroD1) were examined during the 
      differentiation procedure on days 0, 7, 14, 21, and 28 after induction, by using 
      real-time PCR assay. The level of C-peptide and insulin were also measured at the 
      end of the experiment. Dithizone staining showed trans-differentiation of 
      adipose-derived mesenchymal stem cells into pancreatic beta cells evidenced with 
      red-to-brown appearance compared to the control group, indicating the potency to 
      insulin production. These features were at maximum levels 28 days after cell 
      differentiation. Real-time PCR revealed the increase of NeuroD1 and reduction of 
      SOX6 during differentiation of stem cells toward insulin-producing cells (P 
      <0.05). Both miR-101a and miR-107 showed prominent expression at day 28 (P 
      <0.05). Changes in the expression of miR-101a and miR-107coincided with 
      alteration of NeuroD1 and SOX6 that could affect mesenchymal stem cells 
      commitment toward insulin-like beta cells.
FAU - Rajabi, Hadi
AU  - Rajabi H
AD  - Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
AD  - Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
FAU - Aslani, Somayeh
AU  - Aslani S
AD  - Department of Clinical Biochemistry, Faculty of Medicine, Hamedan University of 
      Medical Sciences, Hamedan, Iran.
FAU - Rahbarghazi, Reza
AU  - Rahbarghazi R
AD  - Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
LA  - eng
PT  - Journal Article
DEP - 20210522
PL  - Iran
TA  - Int J Mol Cell Med
JT  - International journal of molecular and cellular medicine
JID - 101598104
PMC - PMC8256832
OTO - NOTNLM
OT  - Mesenchymal stem cells
OT  - differentiation
OT  - insulin-producing cells
OT  - microRNAs
COIS- Authors declare that there is no conflict of interest.
EDAT- 2021/07/17 06:00
MHDA- 2021/07/17 06:01
PMCR- 2021/01/01
CRDT- 2021/07/16 06:40
PHST- 2020/08/28 00:00 [received]
PHST- 2021/03/31 00:00 [accepted]
PHST- 2021/07/16 06:40 [entrez]
PHST- 2021/07/17 06:00 [pubmed]
PHST- 2021/07/17 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.22088/IJMCM.BUMS.10.1.68 [doi]
PST - ppublish
SO  - Int J Mol Cell Med. 2021 Winter;10(1):68-74. doi: 10.22088/IJMCM.BUMS.10.1.68. 
      Epub 2021 May 22.