PMID- 34268302
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210717
IS  - 2296-634X (Print)
IS  - 2296-634X (Electronic)
IS  - 2296-634X (Linking)
VI  - 9
DP  - 2021
TI  - SP1-Mediated Upregulation of Long Noncoding RNA ZFAS1 Involved in Non-syndromic 
      Cleft Lip and Palate via Inactivating WNT/beta-Catenin Signaling Pathway.
PG  - 662780
LID - 10.3389/fcell.2021.662780 [doi]
LID - 662780
AB  - Non-syndromic cleft lip and palate (NSCLP) is one of the most common congenital 
      malformations with multifactorial etiology. Although long non-coding RNAs 
      (lncRNAs) have been implicated in the development of lip and palate, their roles 
      in NSCLP are not fully elucidated. This study aimed to investigate how 
      dysregulated lncRNAs contribute to NSCLP. Using lncRNA sequencing, bioinformatics 
      analysis, and clinical tissue sample detection, we identified that lncRNA ZFAS1 
      was significantly upregulated in NSCLP. The upregulation of ZFAS1 mediated by SP1 
      transcription factor (SP1) inhibited expression levels of Wnt family member 4 
      (WNT4) through the binding with CCCTC-binding factor (CTCF), subsequently 
      inactivating the WNT/beta-catenin signaling pathway, which has been reported to play 
      a significant role on the development of lip and palate. Moreover, in vitro, the 
      overexpression of ZFAS1 inhibited cell proliferation and migration in human oral 
      keratinocytes and human umbilical cord mesenchymal stem cells (HUC-MSCs) and also 
      repressed chondrogenic differentiation of HUC-MSCs. In vivo, ZFAS1 suppressed 
      cell proliferation and numbers of chondrocyte in the zebrafish ethmoid plate. In 
      summary, these results indicated that ZFAS1 may be involved in NSCLP by affecting 
      cell proliferation, migration, and chondrogenic differentiation through 
      inactivating the WNT/beta-catenin signaling pathway.
CI  - Copyright (c) 2021 Chen, Jia, Cai, Ye, Wu, Wan, Zhang, Wu, Xu, Guo, Tian, Ma and 
      Ma.
FAU - Chen, Shiyu
AU  - Chen S
AD  - Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
FAU - Jia, Zhonglin
AU  - Jia Z
AD  - State Key Laboratory of Oral Diseases and National Clinical Research Center for 
      Oral Diseases and Department of Cleft Lip and Palate, West China Hospital of 
      Stomatology, Sichuan University, Chengdu, China.
FAU - Cai, Ming
AU  - Cai M
AD  - Department of Oral and Cranio-Maxillofacial Surgery, Shanghai Ninth People's 
      Hospital, College of Stomatology, Shanghai Jiao Tong University School of 
      Medicine, Shanghai, China.
FAU - Ye, Mujie
AU  - Ye M
AD  - Children's Hospital of Fudan University, Shanghai, China.
FAU - Wu, Dandan
AU  - Wu D
AD  - Department of Oral and Cranio-Maxillofacial Surgery, Shanghai Ninth People's 
      Hospital, College of Stomatology, Shanghai Jiao Tong University School of 
      Medicine, Shanghai, China.
FAU - Wan, Teng
AU  - Wan T
AD  - Department of Oral and Cranio-Maxillofacial Surgery, Shanghai Ninth People's 
      Hospital, College of Stomatology, Shanghai Jiao Tong University School of 
      Medicine, Shanghai, China.
FAU - Zhang, Bowen
AU  - Zhang B
AD  - School of Basic Medical Sciences, Fudan University, Shanghai, China.
FAU - Wu, Peixuan
AU  - Wu P
AD  - School of Basic Medical Sciences, Fudan University, Shanghai, China.
FAU - Xu, Yuexin
AU  - Xu Y
AD  - School of Basic Medical Sciences, Fudan University, Shanghai, China.
FAU - Guo, Yuntao
AU  - Guo Y
AD  - Medical Laboratory of Nantong ZhongKe, Nantong, China.
FAU - Tian, Chan
AU  - Tian C
AD  - Center for Reproductive Medicine, Department of Obstetrics and Gynecology, 
      National Clinical Research Center for Obstetrics and Gynecology, Ministry of 
      Education, Beijing, China.
AD  - Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive 
      Technology, Peking University Third Hospital, Beijing, China.
AD  - Key Laboratory of Assisted Reproduction, Peking University, Beijing, China.
FAU - Ma, Duan
AU  - Ma D
AD  - Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
AD  - School of Basic Medical Sciences, Fudan University, Shanghai, China.
AD  - Children's Hospital of Fudan University, Shanghai, China.
FAU - Ma, Jing
AU  - Ma J
AD  - ENT Institute, Department of Facial Plastic and Reconstructive Surgery, Eye & ENT 
      Hospital, Fudan University, Shanghai, China.
LA  - eng
PT  - Journal Article
DEP - 20210629
PL  - Switzerland
TA  - Front Cell Dev Biol
JT  - Frontiers in cell and developmental biology
JID - 101630250
PMC - PMC8275830
OTO - NOTNLM
OT  - CTCF
OT  - NSCLP
OT  - Sp1
OT  - Wnt/beta-catenin pathway
OT  - ZFAS1
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/07/17 06:00
MHDA- 2021/07/17 06:01
PMCR- 2021/01/01
CRDT- 2021/07/16 06:41
PHST- 2021/02/01 00:00 [received]
PHST- 2021/05/27 00:00 [accepted]
PHST- 2021/07/16 06:41 [entrez]
PHST- 2021/07/17 06:00 [pubmed]
PHST- 2021/07/17 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fcell.2021.662780 [doi]
PST - epublish
SO  - Front Cell Dev Biol. 2021 Jun 29;9:662780. doi: 10.3389/fcell.2021.662780. 
      eCollection 2021.