PMID- 34272446
OWN - NLM
STAT- MEDLINE
DCOM- 20211108
LR  - 20211108
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 11
IP  - 1
DP  - 2021 Jul 16
TI  - MCM-2, Ki-67, and EGFR downregulated expression levels in advanced stage 
      laryngeal squamous cell carcinoma.
PG  - 14607
LID - 10.1038/s41598-021-94077-9 [doi]
LID - 14607
AB  - We present the conceptual study investigated the capacity of minichromosome 
      maintenance-2 (MCM-2), Ki-67, and epidermal growth factor receptor (EGFR) to 
      assess the severity and progression of laryngeal squamous cell carcinoma (LSCC) 
      disease and to study the correlations among these markers. A total of 30 patients 
      with LSCC with immunohistochemistry (IHC) staining for MCM-2, Ki-67 and EGFR were 
      examined. Mean expression levels of the three markers were evaluated for 
      comparing between early and advanced stages of LSCC. The mean MCM-2, Ki-67, and 
      EGFR expression levels were significantly decreased in advanced-stage compared 
      with early-stage LSCC. Pearson correlation analysis showed a statistically 
      significant correlation between the MCM-2 and Ki-67. Regarding subgroup analyses, 
      MCM-2, Ki-67, and EGFR showed significant differences between early- and 
      advanced-stage LSCC with non-recurrence, while for the recurrent subgroup LSCC, 
      only MCM-2 revealed a significant difference between early- and advanced-stage 
      LSCC. Altogether, these results support the role for downregulation of MCM-2, 
      Ki-67 and EGFR in advanced-stage LSCC and correlation of MCM-2 and Ki-67 
      expressions that would be a promising strategy to predict prognosis of LSCC 
      including severity and progression. We contextualize our findings and advocate 
      the position of the biological markers, especially MCM-2, as an emerging 
      evaluation tool for LSCC disease.
CI  - (c) 2021. The Author(s).
FAU - Vivatvakin, Sarocha
AU  - Vivatvakin S
AD  - Department of Physiology, Faculty of Medicine, Chulalongkorn University, 1873 
      Rama 4 Road, Patumwan, Bangkok, 10330, Thailand.
FAU - Ratchataswan, Thanaporn
AU  - Ratchataswan T
AD  - Department of Physiology, Faculty of Medicine, Chulalongkorn University, 1873 
      Rama 4 Road, Patumwan, Bangkok, 10330, Thailand.
FAU - Leesutipornchai, Thiratest
AU  - Leesutipornchai T
AD  - Department of Physiology, Faculty of Medicine, Chulalongkorn University, 1873 
      Rama 4 Road, Patumwan, Bangkok, 10330, Thailand.
FAU - Ruangritchankul, Komkrit
AU  - Ruangritchankul K
AD  - Department of Pathology, Faculty of Medicine, Chulalongkorn University, Bangkok, 
      Thailand.
FAU - Keelawat, Somboon
AU  - Keelawat S
AD  - Department of Pathology, Faculty of Medicine, Chulalongkorn University, Bangkok, 
      Thailand.
FAU - Mahattanasakul, Patnarin
AU  - Mahattanasakul P
AD  - Department of Otolaryngology, Head and Neck Surgery, Faculty of Medicine, 
      Chulalongkorn University, Bangkok, Thailand.
AD  - Department of Otolaryngology, Head and Neck Surgery, King Chulalongkorn Memorial 
      Hospital, Thai Red Cross Society, Bangkok, Thailand.
FAU - Bongsebandhu-Phubhakdi, Saknan
AU  - Bongsebandhu-Phubhakdi S
AD  - Department of Physiology, Faculty of Medicine, Chulalongkorn University, 1873 
      Rama 4 Road, Patumwan, Bangkok, 10330, Thailand. saknan@live.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210716
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Ki-67 Antigen)
RN  - EC 2.7.10.1 (EGFR protein, human)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - EC 3.6.4.12 (MCM2 protein, human)
RN  - EC 3.6.4.12 (Minichromosome Maintenance Complex Component 2)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/*metabolism
MH  - Carcinoma, Squamous Cell/diagnosis/*metabolism/pathology
MH  - Disease Progression
MH  - ErbB Receptors/metabolism
MH  - Female
MH  - Follow-Up Studies
MH  - Humans
MH  - Immunohistochemistry
MH  - Ki-67 Antigen/*metabolism
MH  - Laryngeal Neoplasms/diagnosis/*metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - Minichromosome Maintenance Complex Component 2/*metabolism
MH  - Neoplasm Staging
MH  - Prognosis
MH  - Severity of Illness Index
PMC - PMC8285532
COIS- The authors declare no competing interests.
EDAT- 2021/07/18 06:00
MHDA- 2021/11/09 06:00
PMCR- 2021/07/16
CRDT- 2021/07/17 06:06
PHST- 2021/01/25 00:00 [received]
PHST- 2021/07/06 00:00 [accepted]
PHST- 2021/07/17 06:06 [entrez]
PHST- 2021/07/18 06:00 [pubmed]
PHST- 2021/11/09 06:00 [medline]
PHST- 2021/07/16 00:00 [pmc-release]
AID - 10.1038/s41598-021-94077-9 [pii]
AID - 94077 [pii]
AID - 10.1038/s41598-021-94077-9 [doi]
PST - epublish
SO  - Sci Rep. 2021 Jul 16;11(1):14607. doi: 10.1038/s41598-021-94077-9.