PMID- 34274485 OWN - NLM STAT- MEDLINE DCOM- 20210816 LR - 20210816 IS - 1879-0038 (Electronic) IS - 0378-1119 (Linking) VI - 801 DP - 2021 Oct 30 TI - Novel DNA methylation markers of PRRSV-specific antibodies and their intergenerational transmission from pregnant sows to piglets. PG - 145831 LID - S0378-1119(21)00426-1 [pii] LID - 10.1016/j.gene.2021.145831 [doi] AB - The main strategy for preventing porcine reproductive and respiratory syndrome (PRRS) is vaccination. However, current commercial porcine reproductive and respiratory syndrome virus (PRRSV) vaccines have limited effectiveness and may even cause infections in pigs. The identification of stable molecular markers associated with immune responses to PRRSV vaccination in pigs provides a new approach for PRRS prevention. DNA methylation, the most stable epigenetic molecular marker related to PRRSV vaccination, has not been investigated. In the current research, we used whole genome bisulfite sequencing (WGBS) to investigate DNA methylation in pregnant sows that received PRRSV vaccination and their piglets with high and low PRRSV-specific antibody levels. By performing methylation data analysis and basing on our previous transcriptomic studies, we identified several differentially methylated genes (DMGs) that are involved in the pathways of inflammatory and immune responses. Among the DMGs, ISG15, MX1, SERPINE1, GNG11 and IFIT3 were common hub genes in the two generations. MX1 and GNG11 were located in quantitative trait loci related with PRRSV antibody titer and PRRSV susceptibility, respectively. These results suggest that PRRSV vaccination in sows induces DNA methylation changes in genes and DNA methylation changes occur through intergenerational transmission. The novel DNA methylation markers and target genes observed in our study provide new insights into the molecular mechanisms of immune responses to PRRSV vaccination across two pig generations. CI - Copyright (c) 2021 The Authors. Published by Elsevier B.V. All rights reserved. FAU - Zhang, Fengxia AU - Zhang F AD - National Engineering Laboratory for Animal Breeding, MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, China; Innovation Team of Pig Health Breeding, Institute of Animal Husbandry and Veterinary, Tianjin Academy of Agricultural Sciences, Tianjin, China. FAU - Chen, Siqian AU - Chen S AD - National Engineering Laboratory for Animal Breeding, MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, China. FAU - Yang, Ting AU - Yang T AD - National Engineering Laboratory for Animal Breeding, MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, China. FAU - Ao, Hong AU - Ao H AD - The State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China. FAU - Zhai, Liwei AU - Zhai L AD - National Engineering Laboratory for Animal Breeding, MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, China. FAU - Li, Qianjun AU - Li Q AD - Innovation Team of Pig Health Breeding, Institute of Animal Husbandry and Veterinary, Tianjin Academy of Agricultural Sciences, Tianjin, China. FAU - Xing, Kai AU - Xing K AD - Animal Science and Technology College, Beijing University of Agriculture, Beijing, China. FAU - Liu, Yibing AU - Liu Y AD - National Engineering Laboratory for Animal Breeding, MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, China. FAU - Liu, Huatao AU - Liu H AD - National Engineering Laboratory for Animal Breeding, MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, China. FAU - Yu, Ying AU - Yu Y AD - National Engineering Laboratory for Animal Breeding, MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, China. Electronic address: yuying@cau.edu.cn. FAU - Wang, Chuduan AU - Wang C AD - National Engineering Laboratory for Animal Breeding, MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, China Agricultural University, Beijing, China. Electronic address: cdwang@cau.edu.cn. LA - eng PT - Journal Article DEP - 20210716 PL - Netherlands TA - Gene JT - Gene JID - 7706761 RN - 0 (Antibodies, Viral) RN - 0 (Viral Vaccines) SB - IM MH - Animals MH - Animals, Newborn/immunology/virology MH - Antibodies, Viral/*blood/genetics MH - *DNA Methylation MH - Female MH - Gene Expression Regulation MH - Gene Ontology MH - Infectious Disease Transmission, Vertical MH - Porcine Reproductive and Respiratory Syndrome/*genetics/immunology/transmission MH - Pregnancy MH - Pregnancy, Animal MH - Protein Interaction Maps/genetics/immunology MH - Quantitative Trait Loci MH - Swine MH - Viral Vaccines/*immunology OTO - NOTNLM OT - DNA methylation OT - Intergenerational transmission OT - PRRSV-specific antibody OT - Pig EDAT- 2021/07/19 06:00 MHDA- 2021/08/17 06:00 CRDT- 2021/07/18 20:37 PHST- 2020/11/13 00:00 [received] PHST- 2021/07/01 00:00 [revised] PHST- 2021/07/13 00:00 [accepted] PHST- 2021/07/19 06:00 [pubmed] PHST- 2021/08/17 06:00 [medline] PHST- 2021/07/18 20:37 [entrez] AID - S0378-1119(21)00426-1 [pii] AID - 10.1016/j.gene.2021.145831 [doi] PST - ppublish SO - Gene. 2021 Oct 30;801:145831. doi: 10.1016/j.gene.2021.145831. Epub 2021 Jul 16.