PMID- 34275826
OWN - NLM
STAT- MEDLINE
DCOM- 20220120
LR  - 20220120
IS  - 1011-601X (Print)
IS  - 1011-601X (Linking)
VI  - 34
IP  - 2
DP  - 2021 Mar
TI  - Antioxidant and haematinic effects of methanolic and aqueous methanolic roots 
      extracts of Rauwolfia serpentina Benth in type 2 diabetic mice.
PG  - 529-535
AB  - Present investigation was carried out to evaluate the antioxidant and haematinic 
      effects of methanolic (MREt) and aqueous methanolic (AqMREt) root extracts of R. 
      serpentina in mice model of type 2 diabetes (T2D). Experimental mice were divided 
      into nine groups (six per group) as: fructose-induced (T2D) diabetic group 
      (distilled water 1ml/kg), negative control (0.05% DMSO 1ml/kg), positive control 
      (pioglitazone 15mg/kg) and six test groups (MREt 10, 30 & 60mg/kg & AqMREt 50, 
      100 & 150mg/kg). Whereas tenth group was served as normal control (1ml/kg 
      distilled water). All test doses of MREt & AqMREt significantly (p<0.05) 
      decreases the percent inhibition of catalase (CAT) and superoxide dismutase (SOD) 
      when compared with diabetic controls. Treatment with both extracts also improved 
      the total hemoglobin (Hb), red blood cell (RBC), white blood cell (WBC) counts, 
      packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular 
      hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) in test 
      groups. Fourier transform infrared (FTIR) spectral analysis revealed the presence 
      of phenols moiety in both extracts. Findings suggested that AqMREt possesses more 
      antioxidant and haematinic potential while the MREt of R. serpentina moderately 
      possesses the same activities, which might be due to the high content of phenols 
      present in AqMREt.
FAU - Azmi, Muhammad Bilal
AU  - Azmi MB
AD  - Department of Biochemistry, Dow Medical College, Dow University of Health 
      Sciences, Karachi, Pakistan/ Department of Biochemistry, University of Karachi, 
      Karachi, Pakistan.
FAU - A Qureshi, Shamim
AU  - A Qureshi S
AD  - Department of Biochemistry, Dow Medical College, Dow University of Health 
      Sciences, Karachi, Pakistan.
FAU - Ahmed, Shakil
AU  - Ahmed S
AD  - Industrial Analytical Center, HEJ Research Institute of Chemistry, International 
      Center for Chemical and Biological Sciences, University of Karachi, Karachi, 
      Pakistan.
FAU - Sultana, Saleha
AU  - Sultana S
AD  - Department of Biochemistry, Dow Medical College, Dow University of Health 
      Sciences, Karachi, Pakistan.
FAU - Khan, Auwais Ahmed
AU  - Khan AA
AD  - Department of Pharmacology, Dow College of Pharmacy, Dow University of Health 
      Sciences, Karachi, Pakistan.
FAU - Mudassir, Hina Akram
AU  - Mudassir HA
AD  - Department of Biochemistry, Federal Urdu University of Arts, Science and 
      Technology, Karachi, Pakistan.
LA  - eng
PT  - Journal Article
PL  - Pakistan
TA  - Pak J Pharm Sci
JT  - Pakistan journal of pharmaceutical sciences
JID - 9426356
RN  - 0 (Antioxidants)
RN  - 0 (Hematinics)
RN  - 0 (Hemoglobins)
RN  - 0 (Plant Extracts)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Catalase/drug effects/metabolism
MH  - Diabetes Mellitus, Experimental/*blood
MH  - Diabetes Mellitus, Type 2/*blood
MH  - Erythrocyte Count
MH  - Erythrocyte Indices/*drug effects
MH  - Hematinics/*pharmacology
MH  - Hematocrit
MH  - Hemoglobins/drug effects/metabolism
MH  - Leukocyte Count
MH  - Mice
MH  - Plant Extracts/*pharmacology
MH  - *Plant Roots
MH  - *Rauwolfia
MH  - Superoxide Dismutase/drug effects/metabolism
EDAT- 2021/07/20 06:00
MHDA- 2022/01/21 06:00
CRDT- 2021/07/19 05:42
PHST- 2021/07/19 05:42 [entrez]
PHST- 2021/07/20 06:00 [pubmed]
PHST- 2022/01/21 06:00 [medline]
PST - ppublish
SO  - Pak J Pharm Sci. 2021 Mar;34(2):529-535.