PMID- 34276693
OWN - NLM
STAT- MEDLINE
DCOM- 20211210
LR  - 20211214
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 12
DP  - 2021
TI  - Circulating Plasmacytoid and Conventional Dendritic Cells Are Numerically and 
      Functionally Deficient in Patients With Scrub Typhus.
PG  - 700755
LID - 10.3389/fimmu.2021.700755 [doi]
LID - 700755
AB  - BACKGROUND: Dendritic cells (DCs) are specialized antigen-presenting cells known 
      to bridge innate and adaptive immune reactions. However, the relationship between 
      circulating DCs and Orientia tsutsugamushi infection is unclear. Therefore, this 
      study aimed to examine the level and function of plasmacytoid DCs (pDCs) and 
      conventional DCs (cDCs), two subsets of circulating DCs, in scrub typhus 
      patients. METHODS: The study included 35 scrub typhus patients and 35 healthy 
      controls (HCs). pDC and cDC levels, CD86 and CD274 expression, and cytokine 
      levels were measured using flow cytometry. RESULTS: Circulating pDC and cDC 
      levels were found to be significantly reduced in scrub typhus patients, which 
      were correlated with disease severity. The patients displayed increased 
      percentages of CD86(+) pDCs, CD274(+) pDCs, and CD274(+) cDCs in the peripheral 
      blood. The alterations in the levels and surface phenotypes of pDCs and cDCs were 
      recovered in the remission state. In addition, the production of interferon 
      (IFN)-alpha and tumor necrosis factor (TNF)-alpha by circulating pDCs, and interleukin 
      (IL)-12 and TNF-alpha by circulating cDCs was reduced in scrub typhus patients. 
      Interestingly, our in vitro experiments showed that the percentages of CD86(+) 
      pDCs, CD274(+) pDCs, and CD274(+) cDCs were increased in cultures treated with 
      cytokines including IFN-gamma, IL-12, and TNF-alpha. CONCLUSIONS: This study demonstrates 
      that circulating pDCs and cDCs are numerically deficient and functionally 
      impaired in scrub typhus patients. In addition, alterations in the expression 
      levels of surface phenotypes of pDCs and cDCs could be affected by 
      pro-inflammatory cytokines.
CI  - Copyright (c) 2021 Kang, Park, Jin, Cho, Oh, Kim, Kim, Park, Jung, Kim, Kim, Jo, 
      Ju, Kee and Park.
FAU - Kang, Seung-Ji
AU  - Kang SJ
AD  - Department of Infectious Diseases, Chonnam National University Medical School and 
      Hospital, Gwangju, South Korea.
FAU - Park, Ki-Jeong
AU  - Park KJ
AD  - Department of Rheumatology, Chonnam National University Medical School and 
      Hospital, Gwangju, South Korea.
FAU - Jin, Hye-Mi
AU  - Jin HM
AD  - Department of Rheumatology, Chonnam National University Medical School and 
      Hospital, Gwangju, South Korea.
FAU - Cho, Young-Nan
AU  - Cho YN
AD  - Department of Rheumatology, Chonnam National University Medical School and 
      Hospital, Gwangju, South Korea.
FAU - Oh, Tae Hoon
AU  - Oh TH
AD  - Department of Infectious Diseases, Chonnam National University Medical School and 
      Hospital, Gwangju, South Korea.
FAU - Kim, Seong Eun
AU  - Kim SE
AD  - Department of Infectious Diseases, Chonnam National University Medical School and 
      Hospital, Gwangju, South Korea.
FAU - Kim, Uh Jin
AU  - Kim UJ
AD  - Department of Infectious Diseases, Chonnam National University Medical School and 
      Hospital, Gwangju, South Korea.
FAU - Park, Kyung-Hwa
AU  - Park KH
AD  - Department of Infectious Diseases, Chonnam National University Medical School and 
      Hospital, Gwangju, South Korea.
FAU - Jung, Sook-In
AU  - Jung SI
AD  - Department of Infectious Diseases, Chonnam National University Medical School and 
      Hospital, Gwangju, South Korea.
FAU - Kim, Tae-Ok
AU  - Kim TO
AD  - Department of Pulmonology, Chonnam National University Medical School and 
      Hospital, Gwangju, South Korea.
FAU - Kim, Hyo Shin
AU  - Kim HS
AD  - Department of Surgery, Chonnam National University Medical School and Hospital, 
      Gwangju, South Korea.
FAU - Jo, Young-Goun
AU  - Jo YG
AD  - Department of Surgery, Chonnam National University Medical School and Hospital, 
      Gwangju, South Korea.
FAU - Ju, Jae Kyun
AU  - Ju JK
AD  - Department of Surgery, Chonnam National University Medical School and Hospital, 
      Gwangju, South Korea.
FAU - Kee, Seung-Jung
AU  - Kee SJ
AD  - Department of Laboratory Medicine, Chonnam National University Medical School and 
      Hospital, Gwangju, South Korea.
FAU - Park, Yong-Wook
AU  - Park YW
AD  - Department of Rheumatology, Chonnam National University Medical School and 
      Hospital, Gwangju, South Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210701
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
SB  - IM
MH  - Adult
MH  - Aged
MH  - Cohort Studies
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Scrub Typhus/*immunology
PMC - PMC8281928
OTO - NOTNLM
OT  - Orientia tsutsugamushi
OT  - conventional dendritic cells.
OT  - cytokine
OT  - plasmacytoid dendritic cells
OT  - scrub typhus
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/07/20 06:00
MHDA- 2021/12/15 06:00
PMCR- 2021/01/01
CRDT- 2021/07/19 05:52
PHST- 2021/04/26 00:00 [received]
PHST- 2021/06/21 00:00 [accepted]
PHST- 2021/07/19 05:52 [entrez]
PHST- 2021/07/20 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2021.700755 [doi]
PST - epublish
SO  - Front Immunol. 2021 Jul 1;12:700755. doi: 10.3389/fimmu.2021.700755. eCollection 
      2021.