PMID- 34277611
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220424
IS  - 2296-634X (Print)
IS  - 2296-634X (Electronic)
IS  - 2296-634X (Linking)
VI  - 9
DP  - 2021
TI  - CircSNHG5 Sponges Mir-495-3p and Modulates CITED2 to Protect Cartilage Endplate 
      From Degradation.
PG  - 668715
LID - 10.3389/fcell.2021.668715 [doi]
LID - 668715
AB  - BACKGROUND: Intervertebral disc degeneration (IDD) is a highly prevalent 
      degenerating disease that produces tremendous amount of low back and neck pain. 
      The cartilage endplate (CEP) is vitally important to intervertebral discs in both 
      physiological and pathological conditions. In addition, circular RNAs (circRNAs) 
      have been shown to be involved in the regulation of various diseases, including 
      IDD. However, the particular role of circRNAs in cervical vertebral CEP 
      degeneration remains unclear. Here, we examined the unique role of circRNAs in 
      CEP of patients with cervical fracture and degenerative cervical myelopathy 
      (DCM). METHODS: Human competitive endogenous RNA (ceRNA) microarray was performed 
      by previous research. Western blot (WB), immunofluorescence (IF), quantitative 
      RT-PCR (qRT-PCR), luciferase assay, and fluorescence in situ hybridization (FISH) 
      were employed to analyze the function of circSNHG5 and its downstream effectors, 
      miR-495-3p, and CITED2. RESULTS: We demonstrated that circSNHG5 expression was 
      substantially low in degenerative CEP tissues. Knockdown of circSNHG5 in 
      chondrocytes resulted in a loss of cell proliferation and followed by degradation 
      of extracellular matrix (ECM). In addition, circSNHG5 was shown to sponge 
      miR-495-3p and modulate the expression of the downstream gene CITED2. This 
      mechanism of action was further validated via overexpression and knockdown of 
      CITED2. CONCLUSION: Our findings identified a novel circSNHG5-miR-495-3p axis 
      responsible for IDD progression. Future investigations into IDD therapy may 
      benefit from targeting this axis.
CI  - Copyright (c) 2021 Zhang, Hu, Ding, Yuan, Jia, Wu and Cheng.
FAU - Zhang, Jian
AU  - Zhang J
AD  - Department of Orthopedics, The Second Affiliated Hospital of Nanchang University, 
      Nanchang, China.
AD  - Institute of Orthopedics of Jiangxi Province, Nanchang, China.
AD  - Institute of Minimally Invasive Orthopedics, Nanchang University, Nanchang, 
      China.
FAU - Hu, Shen
AU  - Hu S
AD  - Department of Orthopedics, The Second Affiliated Hospital of Nanchang University, 
      Nanchang, China.
AD  - Institute of Orthopedics of Jiangxi Province, Nanchang, China.
AD  - Institute of Minimally Invasive Orthopedics, Nanchang University, Nanchang, 
      China.
FAU - Ding, Rui
AU  - Ding R
AD  - Department of Orthopedics, The Second Affiliated Hospital of Nanchang University, 
      Nanchang, China.
AD  - Institute of Orthopedics of Jiangxi Province, Nanchang, China.
AD  - Institute of Minimally Invasive Orthopedics, Nanchang University, Nanchang, 
      China.
FAU - Yuan, Jinghong
AU  - Yuan J
AD  - Department of Orthopedics, The Second Affiliated Hospital of Nanchang University, 
      Nanchang, China.
AD  - Institute of Orthopedics of Jiangxi Province, Nanchang, China.
AD  - Institute of Minimally Invasive Orthopedics, Nanchang University, Nanchang, 
      China.
FAU - Jia, Jingyu
AU  - Jia J
AD  - Department of Orthopedics, The Second Affiliated Hospital of Nanchang University, 
      Nanchang, China.
AD  - Institute of Orthopedics of Jiangxi Province, Nanchang, China.
AD  - Institute of Minimally Invasive Orthopedics, Nanchang University, Nanchang, 
      China.
FAU - Wu, Tianlong
AU  - Wu T
AD  - Department of Orthopedics, The Second Affiliated Hospital of Nanchang University, 
      Nanchang, China.
AD  - Institute of Orthopedics of Jiangxi Province, Nanchang, China.
AD  - Institute of Minimally Invasive Orthopedics, Nanchang University, Nanchang, 
      China.
FAU - Cheng, Xigao
AU  - Cheng X
AD  - Department of Orthopedics, The Second Affiliated Hospital of Nanchang University, 
      Nanchang, China.
AD  - Institute of Orthopedics of Jiangxi Province, Nanchang, China.
AD  - Institute of Minimally Invasive Orthopedics, Nanchang University, Nanchang, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20210701
PL  - Switzerland
TA  - Front Cell Dev Biol
JT  - Frontiers in cell and developmental biology
JID - 101630250
PMC - PMC8281349
OTO - NOTNLM
OT  - cartilage endplate
OT  - circular RNA
OT  - competing endogenous RNA
OT  - intervertebral disc degeneration
OT  - microRNA
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/07/20 06:00
MHDA- 2021/07/20 06:01
PMCR- 2021/01/01
CRDT- 2021/07/19 06:00
PHST- 2021/02/17 00:00 [received]
PHST- 2021/05/27 00:00 [accepted]
PHST- 2021/07/19 06:00 [entrez]
PHST- 2021/07/20 06:00 [pubmed]
PHST- 2021/07/20 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fcell.2021.668715 [doi]
PST - epublish
SO  - Front Cell Dev Biol. 2021 Jul 1;9:668715. doi: 10.3389/fcell.2021.668715. 
      eCollection 2021.