PMID- 34281187
OWN - NLM
STAT- MEDLINE
DCOM- 20210806
LR  - 20210806
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 22
IP  - 13
DP  - 2021 Jul 1
TI  - Best Practices and Progress in Precision-Cut Liver Slice Cultures.
LID - 10.3390/ijms22137137 [doi]
LID - 7137
AB  - Thirty-five years ago, precision-cut liver slices (PCLS) were described as a 
      promising tool and were expected to become the standard in vitro model to study 
      liver disease as they tick off all characteristics of a good in vitro model. In 
      contrast to most in vitro models, PCLS retain the complex 3D liver structures 
      found in vivo, including cell-cell and cell-matrix interactions, and therefore 
      should constitute the most reliable tool to model and to investigate pathways 
      underlying chronic liver disease in vitro. Nevertheless, the biggest disadvantage 
      of the model is the initiation of a procedure-induced fibrotic response. In this 
      review, we describe the parameters and potential of PCLS cultures and discuss 
      whether the initially described limitations and pitfalls have been overcome. We 
      summarize the latest advances in PCLS research and critically evaluate PCLS use 
      and progress since its invention in 1985.
FAU - Dewyse, Liza
AU  - Dewyse L
AD  - Liver Cell Biology Research Group, Department of Basic Biomedical Sciences, Vrije 
      Universiteit Brussel, 1090 Brussels, Belgium.
FAU - Reynaert, Hendrik
AU  - Reynaert H
AD  - Department of Gastro-Enterology and Hepatology, Universitair Ziekenhuis Brussel, 
      1090 Brussels, Belgium.
FAU - van Grunsven, Leo A
AU  - van Grunsven LA
AD  - Liver Cell Biology Research Group, Department of Basic Biomedical Sciences, Vrije 
      Universiteit Brussel, 1090 Brussels, Belgium.
LA  - eng
GR  - G042719N/Fonds Wetenschappelijk Onderzoek/
GR  - AAP/Vrije Universiteit Brussel/
PT  - Journal Article
PT  - Review
DEP - 20210701
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
SB  - IM
MH  - Animals
MH  - Humans
MH  - Liver/cytology/metabolism/*pathology
MH  - Liver Diseases/metabolism/pathology
MH  - Microtomy/*methods
MH  - Models, Biological
MH  - Practice Guidelines as Topic
MH  - Tissue Culture Techniques/*methods
PMC - PMC8267882
OTO - NOTNLM
OT  - 3D
OT  - PCLS
OT  - chronic liver disease
OT  - in vitro liver
COIS- The authors declare no conflict of interest.
EDAT- 2021/07/21 06:00
MHDA- 2021/08/07 06:00
PMCR- 2021/07/01
CRDT- 2021/07/20 01:03
PHST- 2021/05/31 00:00 [received]
PHST- 2021/06/25 00:00 [revised]
PHST- 2021/06/30 00:00 [accepted]
PHST- 2021/07/20 01:03 [entrez]
PHST- 2021/07/21 06:00 [pubmed]
PHST- 2021/08/07 06:00 [medline]
PHST- 2021/07/01 00:00 [pmc-release]
AID - ijms22137137 [pii]
AID - ijms-22-07137 [pii]
AID - 10.3390/ijms22137137 [doi]
PST - epublish
SO  - Int J Mol Sci. 2021 Jul 1;22(13):7137. doi: 10.3390/ijms22137137.