PMID- 34281481
OWN - NLM
STAT- MEDLINE
DCOM- 20211213
LR  - 20220204
IS  - 2165-5987 (Electronic)
IS  - 2165-5979 (Print)
IS  - 2165-5979 (Linking)
VI  - 12
IP  - 1
DP  - 2021 Dec
TI  - Delphinidin-3-sambubioside from Hibiscus sabdariffa. L attenuates hyperlipidemia 
      in high fat diet-induced obese rats and oleic acid-induced steatosis in HepG2 
      cells.
PG  - 3837-3849
LID - 10.1080/21655979.2021.1950259 [doi]
AB  - Hibiscus sabdariffa. L is folk medicine that is often used for its hypolipidemic 
      and antihypertensive effects; however, the active compound responsible for its 
      anti-obesity effect is presently unknown. Delphinidin-3-sambubioside (Dp3-Sam) is 
      an anthocyanin, was extracted from Hibiscus sabdariffa L. The present research 
      aimed to investigate the role of Dp3-Sam in the prevention of hyperlipidemia in 
      vivo and in vitro. Rats were fed with a standard chow diet (Control group) or 
      high-fat diet (HFD and DP group) for eight weeks. Besides, HepG2 cells were 
      stimulated with 0.2 mM oleic acid, with or without Dp3-Sam (100-200 microg/ml). Lipid 
      profiles were measured by commercial kits. Oil Red O staining was performed to 
      measure the hepatic and intracellular lipid levels. The key genes of lipid 
      metabolism were measured by RT-PCR. In HFD-fed rats, Dp3-Sam reduced the body 
      weight gain, visceral fat, and abdominal fat and decreased hepatic lipid 
      deposits. Dp3-Sam decreased intracellular TG levels and lipid accumulation in 
      oleic acid-treated HepG2 cells. Besides, Dp3-Sam downregulated the mRNA 
      expression of HMG-CoA reductase (HMGCR), sterol regulatory element-binding 
      protein-1 c (SREBP-1 C), fatty acid synthase (FASN), and acetyl-CoA carboxylase 
      (ACC) and upregulated the mRNA expression of cholesterol 7alpha-hydroxylase (CYP7A1), 
      carnitine palmitoyltransferase1 (CPT1), acyl-coenzyme A oxidase (ACOX), and 
      peroxisome proliferator-activated receptor alpha (PPARalpha). Dp3-Sam up-regulated 
      the expression of phosphorylation of AMP-activated protein kinase (pAMPK) in 
      HFD-fed rats. Our findings indicated that Dp3-Sam possesses the potential to 
      improve lipid metabolism dysfunction and our results offered evidence for the use 
      of Dp3-Sam as therapy for the prevention of obesity and dyslipidemia.
FAU - Long, Qionghua
AU  - Long Q
AD  - Department of General Medicine, Yanan Hospital of Kunming, Kunming, China.
FAU - Chen, Hongyan
AU  - Chen H
AD  - Department of General Medicine, Yanan Hospital of Kunming, Kunming, China.
FAU - Yang, Wenhui
AU  - Yang W
AD  - Department of General Medicine, Yanan Hospital of Kunming, Kunming, China.
FAU - Yang, Li
AU  - Yang L
AD  - Department of General Medicine, Yanan Hospital of Kunming, Kunming, China.
FAU - Zhang, Lijuan
AU  - Zhang L
AUID- ORCID: 0000-0002-3586-8585
AD  - Department of Neurology, Yanan Hospital of Kunming, Kunming, China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Bioengineered
JT  - Bioengineered
JID - 101581063
RN  - 0 (Anthocyanins)
RN  - 0 (delphinidin 3-sambubioside)
RN  - 2UMI9U37CP (Oleic Acid)
SB  - IM
MH  - Animals
MH  - Anthocyanins/*pharmacology
MH  - Diet, High-Fat/adverse effects
MH  - Fatty Liver/metabolism
MH  - Hep G2 Cells
MH  - *Hibiscus
MH  - Humans
MH  - Hyperlipidemias/*metabolism
MH  - Lipid Metabolism/*drug effects
MH  - Liver/drug effects
MH  - Obesity/*metabolism
MH  - Oleic Acid
MH  - Rats
MH  - Rats, Sprague-Dawley
PMC - PMC8806893
OTO - NOTNLM
OT  - Hibiscus sabdariffa
OT  - diet-induced obesity
OT  - hyperlipidemia
OT  - lipid metabolism
COIS- No potential conflict of interest was reported by the author(s).
EDAT- 2021/07/21 06:00
MHDA- 2021/12/15 06:00
PMCR- 2021/07/20
CRDT- 2021/07/20 05:31
PHST- 2021/07/20 05:31 [entrez]
PHST- 2021/07/21 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2021/07/20 00:00 [pmc-release]
AID - 1950259 [pii]
AID - 10.1080/21655979.2021.1950259 [doi]
PST - ppublish
SO  - Bioengineered. 2021 Dec;12(1):3837-3849. doi: 10.1080/21655979.2021.1950259.