PMID- 34282004
OWN - NLM
STAT- MEDLINE
DCOM- 20210809
LR  - 20210809
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Linking)
VI  - 207
IP  - 3
DP  - 2021 Aug 1
TI  - Single-Cell Analysis Reveals the Heterogeneity of Monocyte-Derived and Peripheral 
      Type-2 Conventional Dendritic Cells.
PG  - 837-848
LID - 10.4049/jimmunol.2100094 [doi]
AB  - Dendritic cells (DCs) are critical for pathogen recognition and Ag 
      processing/presentation. Human monocyte-derived DCs (moDCs) have been extensively 
      used in experimental studies and DC-based immunotherapy approaches. However, the 
      extent of human moDC and peripheral DCs heterogeneity and their interrelationship 
      remain elusive. In this study, we performed single-cell RNA sequencing of human 
      moDCs and blood DCs. We identified seven subtypes within moDCs: five corresponded 
      to type 2 conventional DCs (cDC2s), and the other two were CLEC10A(+)CD127(+) 
      cells with no resemblance to any peripheral DC subpopulations characterized to 
      date. Moreover, we defined five similar subtypes in human cDC2s, revealed the 
      potential differentiation trajectory among them, and unveiled the transcriptomic 
      differences between moDCs and cDC2s. We further studied the transcriptomic 
      changes of each moDC subtype during maturation, demonstrating SLAMF7 and IL15RA 
      as maturation markers and CLEC10A and SIGLEC10 as markers for immature DCs. These 
      findings will enable more accurate functional/developmental analyses of human 
      cDC2s and moDCs.
CI  - Copyright (c) 2021 by The American Association of Immunologists, Inc.
FAU - Gao, Yuehan
AU  - Gao Y
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen 
      University, Guangzhou, China.
FAU - Li, He
AU  - Li H
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen 
      University, Guangzhou, China.
FAU - Li, Zhaohuai
AU  - Li Z
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen 
      University, Guangzhou, China.
FAU - Xie, Lihui
AU  - Xie L
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen 
      University, Guangzhou, China.
FAU - Liu, Xiuxing
AU  - Liu X
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen 
      University, Guangzhou, China.
FAU - Huang, Zhaohao
AU  - Huang Z
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen 
      University, Guangzhou, China.
FAU - Chen, Binyao
AU  - Chen B
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen 
      University, Guangzhou, China.
FAU - Lin, Xianchai
AU  - Lin X
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen 
      University, Guangzhou, China.
FAU - Wang, Xianggui
AU  - Wang X
AD  - Eye Center of Xiangya Hospital, Central South University, Changsha, China; and 
      suwr3@mail.sysu.edu.cn zhyfeng@mail.sysu.edu.cn wangxg@csu.edu.cn.
AD  - Hunan Key Laboratory of Ophthalmology, Changsha, China.
FAU - Zheng, Yingfeng
AU  - Zheng Y
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen 
      University, Guangzhou, China; suwr3@mail.sysu.edu.cn zhyfeng@mail.sysu.edu.cn 
      wangxg@csu.edu.cn.
FAU - Su, Wenru
AU  - Su W
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen 
      University, Guangzhou, China; suwr3@mail.sysu.edu.cn zhyfeng@mail.sysu.edu.cn 
      wangxg@csu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210719
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (CLEC10A protein, human)
RN  - 0 (Cytokines)
RN  - 0 (IL15RA protein, human)
RN  - 0 (Lectins)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Interleukin-15)
RN  - 0 (SIGLEC10 protein, human)
RN  - 0 (SLAMF7 protein, human)
RN  - 0 (Signaling Lymphocytic Activation Molecule Family)
SB  - IM
MH  - Adult
MH  - Cell Differentiation/genetics
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*physiology
MH  - Female
MH  - Gene Expression Profiling
MH  - Humans
MH  - Lectins/genetics
MH  - Lectins, C-Type/genetics
MH  - Male
MH  - Monocytes/*physiology
MH  - Receptors, Cell Surface/genetics
MH  - Receptors, Interleukin-15/genetics
MH  - Sequence Analysis, RNA
MH  - Signaling Lymphocytic Activation Molecule Family/genetics
MH  - Single-Cell Analysis/*methods
MH  - Th2 Cells/immunology
MH  - Young Adult
EDAT- 2021/07/21 06:00
MHDA- 2021/08/10 06:00
CRDT- 2021/07/20 06:11
PHST- 2021/02/02 00:00 [received]
PHST- 2021/05/24 00:00 [accepted]
PHST- 2021/07/21 06:00 [pubmed]
PHST- 2021/08/10 06:00 [medline]
PHST- 2021/07/20 06:11 [entrez]
AID - jimmunol.2100094 [pii]
AID - 10.4049/jimmunol.2100094 [doi]
PST - ppublish
SO  - J Immunol. 2021 Aug 1;207(3):837-848. doi: 10.4049/jimmunol.2100094. Epub 2021 
      Jul 19.