PMID- 34284090
OWN - NLM
STAT- MEDLINE
DCOM- 20210928
LR  - 20210928
IS  - 1878-3511 (Electronic)
IS  - 1201-9712 (Linking)
VI  - 110
DP  - 2021 Sep
TI  - Circulating IgA/IgG memory B cells against Mycobacterium tuberculosis 
      dormancy-associated antigens Rv2659c and Rv3128c in active and latent 
      tuberculosis.
PG  - 75-82
LID - S1201-9712(21)00589-0 [pii]
LID - 10.1016/j.ijid.2021.07.033 [doi]
AB  - OBJECTIVE: To elucidate the antigenic potential of dormancy-associated antigens 
      Rv2659c and Rv3128c of Mycobacterium tuberculosis by examining the persistence of 
      specific IgG and IgA memory B cells (MBCs) among patients with active 
      tuberculosis (TB), household contacts with latent tuberculosis (LTBI), and an 
      endemic healthy control group. METHODS: Fresh peripheral blood mononuclear cells 
      from the three study groups were used to enumerate the numbers of IgG and IgA 
      MBCs specific to recombinant protein Rv2659c and Rv3128c by ELISpot assay. The 
      composition of MBC subsets IgA(+) and IgG (+) was analyzed by flow cytometry. 
      RESULTS: The number of IgA MBCs specific to antigen Rv2659c was significantly 
      higher in the LTBI group than the TB group. In contrast, no significant 
      difference was found in IgA or IgG MBCs against antigen Rv3128c. The number of 
      IgA(+) MBCs was significantly higher than that of IgG(+) MBCs in the classical 
      MBC subset of the LTBI group. CONCLUSION: The results indicated that the 
      dormancy-associated antigen Rv2659c induced an IgA MBCs response in individuals 
      with latent TB, and IgA(+) classical MBCs formed a major portion of the MBCs 
      subset. This new knowledge will be beneficial for the development of novel TB 
      vaccines and their control of latent TB.
CI  - Copyright (c) 2021 The Author(s). Published by Elsevier Ltd.. All rights reserved.
FAU - Soe, Phyu Thwe
AU  - Soe PT
AD  - Department of Clinical Microbiology and Applied Technology, Faculty of Medical 
      Technology, Mahidol University, Bangkok, Thailand; Department of Medical 
      Laboratory Technology, University of Medical Technology, Mandalay, Myanmar.
FAU - Hanthamrongwit, Jariya
AU  - Hanthamrongwit J
AD  - Department of Clinical Microbiology and Applied Technology, Faculty of Medical 
      Technology, Mahidol University, Bangkok, Thailand.
FAU - Saelee, Chutiphon
AU  - Saelee C
AD  - Department of Clinical Microbiology and Applied Technology, Faculty of Medical 
      Technology, Mahidol University, Bangkok, Thailand.
FAU - Kyaw, Soe Paing
AU  - Kyaw SP
AD  - Clinical Pathology Laboratory, (1000) Bedded General Hospital, Nay Pyi Taw, 
      Myanmar.
FAU - Khaenam, Prasong
AU  - Khaenam P
AD  - Center of Standardization and Product Validation, Faculty of Medical Technology, 
      Mahidol University, Bangkok, Thailand.
FAU - Warit, Saradee
AU  - Warit S
AD  - Industrial Tuberculosis Team, IMMBRG, National Center for Genetic Engineering and 
      Biotechnology (BIOTEC), NSTDA, Pathum Thani, Thailand.
FAU - Satproedprai, Nusara
AU  - Satproedprai N
AD  - Genomic Medicine and Innovation Support Division, Department of Medical Sciences, 
      Ministry of Public Health, Thailand.
FAU - Mahasirimongkol, Surakameth
AU  - Mahasirimongkol S
AD  - Genomic Medicine and Innovation Support Division, Department of Medical Sciences, 
      Ministry of Public Health, Thailand.
FAU - Yanai, Hideki
AU  - Yanai H
AD  - Department of Clinical Laboratory, Fukujuji Hospital, Japan Anti-Tuberculosis 
      Association (JATA), Tokyo, Japan.
FAU - Chootong, Patchanee
AU  - Chootong P
AD  - Department of Clinical Microbiology and Applied Technology, Faculty of Medical 
      Technology, Mahidol University, Bangkok, Thailand.
FAU - Leepiyasakulchai, Chaniya
AU  - Leepiyasakulchai C
AD  - Department of Clinical Microbiology and Applied Technology, Faculty of Medical 
      Technology, Mahidol University, Bangkok, Thailand. Electronic address: 
      chaniya.lee@mahidol.edu.
LA  - eng
PT  - Journal Article
DEP - 20210718
PL  - Canada
TA  - Int J Infect Dis
JT  - International journal of infectious diseases : IJID : official publication of the 
      International Society for Infectious Diseases
JID - 9610933
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Immunoglobulin A)
RN  - 0 (Immunoglobulin G)
SB  - IM
MH  - Antigens, Bacterial
MH  - B-Lymphocytes
MH  - Humans
MH  - Immunoglobulin A
MH  - Immunoglobulin G
MH  - *Latent Tuberculosis
MH  - Leukocytes, Mononuclear
MH  - *Mycobacterium tuberculosis
OTO - NOTNLM
OT  - Latent tuberculosis infection
OT  - Memory B cells
OT  - Rv2659c
OT  - Rv3128c
EDAT- 2021/07/21 06:00
MHDA- 2021/09/29 06:00
CRDT- 2021/07/20 20:12
PHST- 2021/05/19 00:00 [received]
PHST- 2021/07/12 00:00 [revised]
PHST- 2021/07/13 00:00 [accepted]
PHST- 2021/07/21 06:00 [pubmed]
PHST- 2021/09/29 06:00 [medline]
PHST- 2021/07/20 20:12 [entrez]
AID - S1201-9712(21)00589-0 [pii]
AID - 10.1016/j.ijid.2021.07.033 [doi]
PST - ppublish
SO  - Int J Infect Dis. 2021 Sep;110:75-82. doi: 10.1016/j.ijid.2021.07.033. Epub 2021 
      Jul 18.