PMID- 34284381
OWN - NLM
STAT- MEDLINE
DCOM- 20220208
LR  - 20220208
IS  - 1421-9662 (Electronic)
IS  - 0001-5792 (Linking)
VI  - 145
IP  - 1
DP  - 2022
TI  - In vitro Immunomodulatory Effects of Human Umbilical Cord-Derived Mesenchymal 
      Stem Cells on Peripheral Blood Cells from Warm Autoimmune Hemolytic Anemia 
      Patients.
PG  - 63-71
LID - 10.1159/000506759 [doi]
AB  - INTRODUCTION: Autoimmune hemolytic anemia is a potentially lethal disease 
      characterized by autoimmune hemolysis. Although human umbilical cord-derived 
      mesenchymal stem cells (hUC-MSCs) have been reported as a promising therapy, 
      there is limited evidence regarding warm autoimmune hemolytic anemia (wAIHA) 
      patients. This study aimed to investigate the potential therapeutic effects of 
      hUC-MSCs via immune regulation in wAIHA patients. METHODS: Peripheral blood 
      mononuclear cells (PBMCs) from 10 wAIHA patients and 8 healthy controls were 
      isolated from peripheral blood and cultured for 3 days with or without the 
      presence of hUC-MSCs; PBMCs were co-cultured with hUC-MSCs using Transwell 
      assays. The supernatant cytokine levels were measured after culture through 
      AimPlex Multiple Immunoassays for Flow, including IL-2, IL-4, IL-10, IFN-gamma, 
      TNF-alpha, and IL-17A. The percentages of regulatory T cells, regulatory B cells, and 
      Th1/Th2 in PBMCs were also assessed before and after culturing. RESULTS: In the 
      wAIHA group, hUC-MSCs could upregulate the Treg and Breg proportions after 
      culturing for 3 days, and the Treg and Breg percentages increased after 
      co-culturing with hUC-MSCs in the wAIHA group compared with PBMC cultured alone 
      for 3 days (8.29 +/- 8.59 vs. 6.82 +/- 1.32, 3.82 +/- 1.87 vs. 1.75 +/- 1.20, 
      respectively). Compared with the PBMC wAIHA group, the levels of TNF-alpha (2.13 +/- 
      2.07 vs. 16.20 +/- 21.13 pg/mL, p = 0.019) and IL-10 (10.51 +/- 18.42 vs. 37.78 +/- 
      44.20 pg/mL, p = 0.012) were significantly elevated in the PBMC + hUC-MSCs wAIHA 
      group. CONCLUSION: The hUC-MSCs contributed to the increasing proportion of 
      regulatory cell populations in PBMCs of wAIHA patients, thereby potentially 
      regulating autoimmune response; thus, hUC-MSCs may be a promising approach for 
      wAIHA treatment.
CI  - (c) 2021 S. Karger AG, Basel.
FAU - Ma, Chunya
AU  - Ma C
AD  - Department of Blood Transfusion, First Medical Center of Chinese PLA General 
      Hospital, Beijing, China.
FAU - Feng, Yannan
AU  - Feng Y
AD  - Department of Blood Transfusion, First Medical Center of Chinese PLA General 
      Hospital, Beijing, China.
FAU - Yang, Lu
AU  - Yang L
AD  - Department of Blood Transfusion, First Medical Center of Chinese PLA General 
      Hospital, Beijing, China.
FAU - Wang, Shufang
AU  - Wang S
AD  - Department of Blood Transfusion, First Medical Center of Chinese PLA General 
      Hospital, Beijing, China.
FAU - Sun, Xiaolin
AU  - Sun X
AD  - Department of Blood Transfusion, First Medical Center of Chinese PLA General 
      Hospital, Beijing, China.
FAU - Tai, Shengfei
AU  - Tai S
AD  - Department of Blood Transfusion, First Medical Center of Chinese PLA General 
      Hospital, Beijing, China.
FAU - Guan, Xiaozhen
AU  - Guan X
AD  - Department of Blood Transfusion, First Medical Center of Chinese PLA General 
      Hospital, Beijing, China.
FAU - Wang, Deqing
AU  - Wang D
AD  - Department of Blood Transfusion, First Medical Center of Chinese PLA General 
      Hospital, Beijing, China.
FAU - Yu, Yang
AU  - Yu Y
AD  - Department of Blood Transfusion, First Medical Center of Chinese PLA General 
      Hospital, Beijing, China.
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
DEP - 20210720
PL  - Switzerland
TA  - Acta Haematol
JT  - Acta haematologica
JID - 0141053
RN  - 0 (Cytokines)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Anemia, Hemolytic, Autoimmune/*immunology/pathology
MH  - Coculture Techniques
MH  - Cytokines/*immunology
MH  - Female
MH  - Humans
MH  - *Immunomodulation
MH  - Lymphocytes/*immunology/pathology
MH  - Male
MH  - Mesenchymal Stem Cells/*immunology/pathology
MH  - Middle Aged
MH  - Umbilical Cord/*immunology/pathology
OTO - NOTNLM
OT  - Cytokines
OT  - Human umbilical cord-derived mesenchymal stem cells
OT  - Immunomodulation
OT  - Peripheral blood mononuclear cells
OT  - Warm autoimmune hemolytic anemia
EDAT- 2021/07/21 06:00
MHDA- 2022/02/09 06:00
CRDT- 2021/07/20 20:26
PHST- 2019/04/28 00:00 [received]
PHST- 2020/02/24 00:00 [accepted]
PHST- 2021/07/21 06:00 [pubmed]
PHST- 2022/02/09 06:00 [medline]
PHST- 2021/07/20 20:26 [entrez]
AID - 000506759 [pii]
AID - 10.1159/000506759 [doi]
PST - ppublish
SO  - Acta Haematol. 2022;145(1):63-71. doi: 10.1159/000506759. Epub 2021 Jul 20.