PMID- 34288575
OWN - NLM
STAT- MEDLINE
DCOM- 20220228
LR  - 20220801
IS  - 2053-1095 (Electronic)
IS  - 2053-1095 (Linking)
VI  - 7
IP  - 5
DP  - 2021 Sep
TI  - Effects of hydroethanolic extracts of Terminalia chebula and Thymbra spicata on 
      ram fresh semen under normal and oxidative stress conditions.
PG  - 1778-1785
LID - 10.1002/vms3.580 [doi]
AB  - The objective of this study was to evaluate protective effects of hydroethanolic 
      extracts of Terminalia chebula and Thymbra spicata on viability, lipid 
      peroxidation (LPO) and DNA integrity of ram fresh semen under normal and 
      oxidative stress (OS) conditions. Antioxidant activities of different 
      concentrations of Terminalia chebula and Thymbra spicata extracts were evaluated 
      with DPPH assay. Semen samples were taken from three fertile adult rams. After 
      diluting semen with Tris-base extender, different concentrations of Terminalia 
      chebula and Thymbra spicata (30, 300, and 3000 mug/ml) extracts were used under 
      normal and induced OS conditions. The group not receiving any supplements was 
      considered as control group. A total of 50 muM hydrogen peroxide was used to 
      induce OS. MTT solution was added to each of treatment groups which were kept in 
      an incubator at 37 degrees C for 2 h. After incubation, readings were obtained by ELISA 
      reader. DNA integrity and LPO were determined with acridine orange (AO) staining 
      and malondialdehyde (MDA) assay. Higher concentrations of Terminalia chebula and 
      Thymbra spicata extracts preserved viability and DNA integrity while reducing MDA 
      concentrations compared to other treatment groups. Also, under induced OS, higher 
      concentrations of both extracts reduced detrimental effects of H(2) O(2) . In 
      conclusion, it seems that addition of Terminalia chebula and Thymbra spicata 
      extracts can reduce induced OS in spermatozoa.
CI  - (c) 2021 The Authors. Veterinary Medicine and Science published by John Wiley & 
      Sons Ltd.
FAU - Mohammadi, Tayebeh
AU  - Mohammadi T
AUID- ORCID: 0000-0002-8460-8261
AD  - Basic Sciences and Pathobiology Department, Faculty of Veterinary Medicine, Razi 
      University, Kermanshah, Iran.
FAU - Soltani, Leila
AU  - Soltani L
AUID- ORCID: 0000-0002-5007-5110
AD  - Department of Animal Sciences, College of Agriculture and Natural Resources, Razi 
      University, Kermanshah, Iran.
LA  - eng
PT  - Journal Article
DEP - 20210720
PL  - England
TA  - Vet Med Sci
JT  - Veterinary medicine and science
JID - 101678837
RN  - 0 (Antioxidants)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Animals
MH  - Antioxidants/pharmacology
MH  - Oxidative Stress
MH  - Plant Extracts/pharmacology
MH  - *Semen/drug effects
MH  - Sheep
MH  - *Terminalia
PMC - PMC8464289
OTO - NOTNLM
OT  - Terminalia chebula
OT  - Thymbra spicata
OT  - oxidative stress
OT  - ram
OT  - spermatozoa
COIS- The authors declare no conflict of interest.
EDAT- 2021/07/22 06:00
MHDA- 2022/03/01 06:00
PMCR- 2021/07/20
CRDT- 2021/07/21 13:54
PHST- 2021/07/22 06:00 [pubmed]
PHST- 2022/03/01 06:00 [medline]
PHST- 2021/07/21 13:54 [entrez]
PHST- 2021/07/20 00:00 [pmc-release]
AID - VMS3580 [pii]
AID - 10.1002/vms3.580 [doi]
PST - ppublish
SO  - Vet Med Sci. 2021 Sep;7(5):1778-1785. doi: 10.1002/vms3.580. Epub 2021 Jul 20.