PMID- 34289257
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210925
IS  - 2578-5745 (Electronic)
IS  - 2578-5745 (Linking)
VI  - 3
IP  - 9
DP  - 2021 Sep
TI  - Serum MMP3 Correlated With IL1beta Messenger RNA Expressions of Peripheral Blood 
      Mononuclear Cells in Patients With Relapsing Polychondritis With Respiratory 
      Involvement.
PG  - 636-641
LID - 10.1002/acr2.11301 [doi]
AB  - OBJECTIVE: Respiratory involvement was intimately associated with poorer 
      prognosis in patients with relapsing polychondritis (RP). We previously reported 
      that high serum matrix metalloproteinase-3 (MMP3) was frequently observed in 
      patients with RP with respiratory involvement. Elevated MMP3 secreted through 
      local inflammation may be associated with the development of airway lesions. 
      METHODS: We collected peripheral blood mononuclear cells (PBMCs) and sera from 30 
      patients with RP and 14 healthy individuals. Interleukin (IL) 1beta, IL6, and tumor 
      necrosis factor (TNF) alpha messenger RNA (mRNA) expressions were analyzed in freshly 
      isolated and cultured PBMCs with phytohemagglutinin and phorbol myristate acetate 
      stimulation by real-time reverse transcription polymerase chain reaction and 
      serum MMP3 by enzyme-linked immunosorbent assay (ELISA). RESULTS: We confirmed 
      our previous finding that patients with respiratory involvements showed higher 
      serum MMP3 compared with patients lacking respiratory involvement. IL1beta mRNA 
      expression was significantly higher in patients with RP than in healthy 
      individuals after mitogenic stimulation. TNFalpha mRNA expression after stimulation 
      was significantly lower in patients with RP compared with in healthy individuals. 
      We performed correlation analyses between MMP3 and cytokine mRNA expressions in 
      patients with RP. In patients with respiratory involvement, MMP3 correlated with 
      IL1beta and IL6 after stimulation. In patients without respiratory involvement, no 
      positive correlations between MMP3 and cytokine mRNA expressions were observed 
      regardless of culture condition. We did not find any positive correlations 
      between MMP3 and TNFalpha mRNA expression in patients with RP. CONCLUSION: It is 
      possible that IL1beta mRNA expression associates by some means with airway 
      inflammation via the secretion of MMP3 in patients with RP. Involvement of 
      proinflammatory cytokines, including IL1beta, was suggested for the pathophysiology 
      of airway lesions in patients with RP.
CI  - (c) 2021 The Authors. ACR Open Rheumatology published by Wiley Periodicals LLC on 
      behalf of American College of Rheumatology.
FAU - Shimizu, Jun
AU  - Shimizu J
AUID- ORCID: 0000-0003-3270-4443
AD  - St. Marianna University School of Medicine, Kawasaki, Japan.
FAU - Wakisaka, Sueshige
AU  - Wakisaka S
AD  - St. Marianna University School of Medicine, Kawasaki, Japan.
FAU - Suzuki, Tomoko
AU  - Suzuki T
AD  - St. Marianna University School of Medicine, Kawasaki, Japan.
FAU - Suzuki, Noboru
AU  - Suzuki N
AD  - St. Marianna University School of Medicine, Kawasaki, Japan.
LA  - eng
GR  - Grants-in-Aid from the Research Committee of Rare Disease, the Ministry of 
      Health, Labor and Welfare of Japan/
PT  - Journal Article
DEP - 20210721
PL  - United States
TA  - ACR Open Rheumatol
JT  - ACR open rheumatology
JID - 101740025
PMC - PMC8449037
EDAT- 2021/07/22 06:00
MHDA- 2021/07/22 06:01
PMCR- 2021/07/21
CRDT- 2021/07/21 17:44
PHST- 2020/11/16 00:00 [received]
PHST- 2021/06/11 00:00 [accepted]
PHST- 2021/07/22 06:00 [pubmed]
PHST- 2021/07/22 06:01 [medline]
PHST- 2021/07/21 17:44 [entrez]
PHST- 2021/07/21 00:00 [pmc-release]
AID - ACR211301 [pii]
AID - 10.1002/acr2.11301 [doi]
PST - ppublish
SO  - ACR Open Rheumatol. 2021 Sep;3(9):636-641. doi: 10.1002/acr2.11301. Epub 2021 Jul 
      21.