PMID- 34289478
OWN - NLM
STAT- MEDLINE
DCOM- 20210917
LR  - 20210917
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 161
IP  - 5
DP  - 2021
TI  - FISH-Based Karyotype Analyses of Four Dracaena Species.
PG  - 272-277
LID - 10.1159/000516897 [doi]
AB  - The genus Dracaena is the main source of dragon's blood, which is a plant resin 
      and has been used as traditional medicine since ancient times in different 
      civilizations. However, the chromosome numbers and karyotypes present in this 
      genus remain poorly understood. In this study, fluorescence in situ hybridization 
      (FISH) using oligonucleotide probes for ribosomal DNAs (5S and 45S rDNA) and 
      telomeric repeats (TTTAGGG)3 was applied to analyze 4 related species: Dracaena 
      terniflora Roxb., Dracaena cambodiana Pierre ex Gagnep., Aizong (Dracaena sp.), 
      and Dracaena cochinchinensis (Lour.) S.C. Chen. In all 4 species, both 5S and 45S 
      rDNA showed hybridization signals in the paracentromeric region of a pair of 
      chromosomes; the sizes of the 45S rDNA signals were larger than those of the 5S 
      rDNA. Importantly, the telomeric repeat signals were located in the telomeric 
      regions of almost all chromosomes. The results indicated that the chromosome 
      number of all 4 Dracaena species is 2n = 40, and the lengths of the mitotic 
      metaphase chromosomes range from 0.99 to 2.98 mum. Our results provide useful 
      cytogenetic information, which will be beneficial to future studies in genome 
      structure of the genus Dracaena.
CI  - (c) 2021 S. Karger AG, Basel.
FAU - Zhao, Hongyou
AU  - Zhao H
AD  - College of Pharmaceutical Science, Yunnan University of Chinese Medicine, 
      Kunming, China.
AD  - Institute of Medicinal Plant Development, Yunnan Branch, Chinese Academy of 
      Medical Sciences & Peking Union Medical College, Jinghong, China.
AD  - Yunnan Key Laboratory of Southern Medicinal Resources, Jinghong, China.
FAU - Li, Shuang
AU  - Li S
AD  - Institute of Medicinal Plant Development, Yunnan Branch, Chinese Academy of 
      Medical Sciences & Peking Union Medical College, Jinghong, China.
AD  - Yunnan Key Laboratory of Southern Medicinal Resources, Jinghong, China.
FAU - Yang, Chunyong
AU  - Yang C
AD  - Institute of Medicinal Plant Development, Yunnan Branch, Chinese Academy of 
      Medical Sciences & Peking Union Medical College, Jinghong, China.
AD  - Yunnan Key Laboratory of Southern Medicinal Resources, Jinghong, China.
FAU - Li, Ge
AU  - Li G
AD  - Institute of Medicinal Plant Development, Yunnan Branch, Chinese Academy of 
      Medical Sciences & Peking Union Medical College, Jinghong, China.
AD  - Yunnan Key Laboratory of Southern Medicinal Resources, Jinghong, China.
FAU - Wang, Yanfang
AU  - Wang Y
AD  - Institute of Medicinal Plant Development, Yunnan Branch, Chinese Academy of 
      Medical Sciences & Peking Union Medical College, Jinghong, China.
AD  - Yunnan Key Laboratory of Southern Medicinal Resources, Jinghong, China.
FAU - Peng, Jianming
AU  - Peng J
AD  - Institute of Medicinal Plant Development, Yunnan Branch, Chinese Academy of 
      Medical Sciences & Peking Union Medical College, Jinghong, China.
AD  - Yunnan Key Laboratory of Southern Medicinal Resources, Jinghong, China.
FAU - Yan, Zhen
AU  - Yan Z
AD  - Institute of Medicinal Plant Development, Yunnan Branch, Chinese Academy of 
      Medical Sciences & Peking Union Medical College, Jinghong, China.
AD  - Yunnan Key Laboratory of Southern Medicinal Resources, Jinghong, China.
FAU - Li, Rongying
AU  - Li R
AD  - Institute of Medicinal Plant Development, Yunnan Branch, Chinese Academy of 
      Medical Sciences & Peking Union Medical College, Jinghong, China.
AD  - Yunnan Key Laboratory of Southern Medicinal Resources, Jinghong, China.
FAU - Wang, Yanqian
AU  - Wang Y
AD  - Institute of Medicinal Plant Development, Yunnan Branch, Chinese Academy of 
      Medical Sciences & Peking Union Medical College, Jinghong, China.
AD  - Yunnan Key Laboratory of Southern Medicinal Resources, Jinghong, China.
FAU - Zhang, Lixia
AU  - Zhang L
AD  - Institute of Medicinal Plant Development, Yunnan Branch, Chinese Academy of 
      Medical Sciences & Peking Union Medical College, Jinghong, China.
AD  - Yunnan Key Laboratory of Southern Medicinal Resources, Jinghong, China.
LA  - eng
PT  - Journal Article
DEP - 20210721
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (RNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 5S)
RN  - 0 (RNA, ribosomal, 45S)
SB  - IM
MH  - Centromere
MH  - China
MH  - Chromosome Mapping/*methods
MH  - Chromosomes, Plant/*chemistry
MH  - Dracaena/classification/*genetics
MH  - In Situ Hybridization, Fluorescence/methods
MH  - *Karyotype
MH  - Karyotyping/methods
MH  - Phylogeography
MH  - RNA, Ribosomal/genetics
MH  - RNA, Ribosomal, 5S/genetics
MH  - Telomere
OTO - NOTNLM
OT  - 5S and 45S rDNA
OT  - Dracaena
OT  - FISH
OT  - Karyotype analysis
OT  - Telomeric repeat
EDAT- 2021/07/22 06:00
MHDA- 2021/09/18 06:00
CRDT- 2021/07/21 20:11
PHST- 2020/12/21 00:00 [received]
PHST- 2021/04/28 00:00 [accepted]
PHST- 2021/07/22 06:00 [pubmed]
PHST- 2021/09/18 06:00 [medline]
PHST- 2021/07/21 20:11 [entrez]
AID - 000516897 [pii]
AID - 10.1159/000516897 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2021;161(5):272-277. doi: 10.1159/000516897. Epub 2021 Jul 
      21.