PMID- 34295778
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220425
IS  - 2224-4344 (Print)
IS  - 2224-4344 (Electronic)
IS  - 2224-4336 (Linking)
VI  - 10
IP  - 6
DP  - 2021 Jun
TI  - Effect of SOCS3 on apoptosis of human trophoblasts via adjustment of the 
      JAK2/STAT3 signaling pathway in preterm birth.
PG  - 1637-1646
LID - 10.21037/tp-21-39 [doi]
AB  - BACKGROUND: The expression of suppressor of cytokine signaling 3 (SOCS3) was 
      induced by interleukin-6 (IL-6) in preterm placental tissues. However, its role 
      in IL-6 induced apoptosis of trophoblast cells derived from preterm placental 
      tissues remains to be elucidated. METHODS: Primary cytotrophoblasts from human 
      preterm placental tissues were used to stably knock down and overexpress the 
      level of SOCS3 by corresponding lentiviral vectors and the expression of SOCS3 
      was validated by quantitative reverse transcription polymerase chain reaction 
      (qRT-PCR) and Western blot. The effect of SOCS3 overexpression or knockdown on 
      the proliferation and apoptosis of IL-6 treated human cytotrophoblasts were 
      determined by Cell Counting Kit-8 (CCK8) assay and Annexin-V/Propidium Iodide 
      (PI) double-staining assay, respectively. Based on it, we detected the proteins 
      associated with the Janus Tyrosine Kinase (JAK)/Signal Transducer and Activator 
      of Transcription (STAT) pathway and apoptosis, such as JAK2, p-JAK2, STAT3, 
      p-STAT3, B-cell lymphoma-2 (Bcl-2) and BCL2-associated X (Bax) by Western blot. 
      RESULTS: IL-6-treatment resulted in significant apoptosis of human 
      cytotrophoblasts. Overexpressing SOCS3 in the cytotrophoblasts reduced cell 
      apoptosis, while the knockdown of SCOS3 had the opposite effects. Further 
      analyses showed that SOCS3 overexpression inhibited JAK2 and STAT3 
      phosphorylation, which was induced by IL-6 stimulation. CONCLUSIONS: SOCS3 plays 
      a protective role in human preterm placental tissue-derived cytotrophoblasts from 
      IL-6 induced apoptosis by feedback inhibition of JAK2/STAT3 signaling.
CI  - 2021 Translational Pediatrics. All rights reserved.
FAU - Yin, Yin
AU  - Yin Y
AD  - Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing 
      Medical University, Nanjing, China.
FAU - Qu, Lin
AU  - Qu L
AD  - Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing 
      Medical University, Nanjing, China.
FAU - Zhu, Dicong
AU  - Zhu D
AD  - State Key Laboratory of Reproductive Medicine, Nanjing Medical University, 
      Nanjing, China.
FAU - Wu, Yang
AU  - Wu Y
AD  - Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing 
      Medical University, Nanjing, China.
FAU - Zhou, Xin
AU  - Zhou X
AD  - Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing 
      Medical University, Nanjing, China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Transl Pediatr
JT  - Translational pediatrics
JID - 101649179
PMC - PMC8261589
OTO - NOTNLM
OT  - JAK2/STAT3 signaling
OT  - Preterm birth
OT  - apoptosis
OT  - interleukin-6 (IL-6)
OT  - suppressor of cytokine signaling 3 (SOCS3)
COIS- Conflicts of Interest: All authors have completed the ICMJE uniform disclosure 
      form (available at http://dx.doi.org/10.21037/tp-21-39). The authors have no 
      conflicts of interest to declare.
EDAT- 2021/07/24 06:00
MHDA- 2021/07/24 06:01
PMCR- 2021/06/01
CRDT- 2021/07/23 06:51
PHST- 2021/01/29 00:00 [received]
PHST- 2021/05/12 00:00 [accepted]
PHST- 2021/07/23 06:51 [entrez]
PHST- 2021/07/24 06:00 [pubmed]
PHST- 2021/07/24 06:01 [medline]
PHST- 2021/06/01 00:00 [pmc-release]
AID - tp-10-06-1637 [pii]
AID - 10.21037/tp-21-39 [doi]
PST - ppublish
SO  - Transl Pediatr. 2021 Jun;10(6):1637-1646. doi: 10.21037/tp-21-39.