PMID- 34295935
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210724
IS  - 2297-1769 (Print)
IS  - 2297-1769 (Electronic)
IS  - 2297-1769 (Linking)
VI  - 8
DP  - 2021
TI  - Effects of a High-Grain Diet With a Buffering Agent on Milk Protein Synthesis in 
      Lactating Goats.
PG  - 696703
LID - 10.3389/fvets.2021.696703 [doi]
LID - 696703
AB  - Chinese dairy industries have developed rapidly, providing consumers with 
      high-quality sources of nutrition. However, many problems have also appeared 
      during the development process, especially the low quality of milk. To improve 
      milk quality, a large amount of concentrated feed is usually added to the diet 
      within a certain period of time, which increases the milk production to a certain 
      extent. However, long-term feeding with high-concentration feed can lead to 
      subacute rumen acidosis. Therefore, the present study aimed to determine the 
      effect of adding a buffer on subacute rumen acidosis, and the improvement of milk 
      production and milk quality. We also aimed to study the mechanism of promoting 
      mammary gland lactation. A total of 12 healthy mid-lactating goats were randomly 
      divided into two groups, they were high-grain diet group (Control) and buffering 
      agent group. To understand the effects of high-grain diets with buffers on amino 
      acids in jugular blood and the effects of amino acids on milk protein synthesis, 
      Milk-Testing Milkoscan 4000, commercial kits, and high-performance liquid 
      chromatography (HPLC) measurements were integrated with the milk protein rate, 
      the amino acid concentration in jugular venous blood samples, quantitative 
      real-time PCR, comparative proteomics, and western blotting to study 
      differentially expressed proteins and amino acids in mammary gland tissues of 
      goats fed high-grain diets. Feeding lactating goats with buffering agent 
      increased the percentage of milk protein in milk, significantly increased the 
      amino acid content of jugular blood (p < 0.05), and increase the amino acid 
      transporter levels in the mammary gland. Compared with the high-grain group, 
      2-dimensional electrophoresis technology, matrix-assisted laser 
      desorption/ionization-time of flight/time of flight proteomics analyzer, and 
      western blot analysis further verified that the expression levels of beta casein 
      (CSN2) and lactoferrin (LF) proteins in the mammary glands of lactating goats 
      were higher when fed a high-grain diets and buffers. The mechanism of increased 
      milk protein synthesis was demonstrated to be related to the activation of 
      mammalian target of rapamycin (mTOR) pathway signals.
CI  - Copyright (c) 2021 He, Nie, Wang, Yan and Zhang.
FAU - He, Meilin
AU  - He M
AD  - The Key Laboratory of Animal Physiology and Biochemistry, Ministry of 
      Agriculture, Nanjing Agricultural University, Nanjing, China.
FAU - Nie, Xintian
AU  - Nie X
AD  - College of Engineering, Nanjing Agricultural University, Nanjing, China.
FAU - Wang, Huanhuan
AU  - Wang H
AD  - The Key Laboratory of Animal Physiology and Biochemistry, Ministry of 
      Agriculture, Nanjing Agricultural University, Nanjing, China.
FAU - Yan, Shuping
AU  - Yan S
AD  - The Key Laboratory of Animal Physiology and Biochemistry, Ministry of 
      Agriculture, Nanjing Agricultural University, Nanjing, China.
FAU - Zhang, Yuanshu
AU  - Zhang Y
AD  - The Key Laboratory of Animal Physiology and Biochemistry, Ministry of 
      Agriculture, Nanjing Agricultural University, Nanjing, China.
LA  - eng
PT  - Journal Article
DEP - 20210706
PL  - Switzerland
TA  - Front Vet Sci
JT  - Frontiers in veterinary science
JID - 101666658
PMC - PMC8291223
OTO - NOTNLM
OT  - HPLC
OT  - amino acids
OT  - buffering agent
OT  - comparative proteomics
OT  - lactating dairy goats
OT  - milk protein
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/07/24 06:00
MHDA- 2021/07/24 06:01
PMCR- 2021/01/01
CRDT- 2021/07/23 06:52
PHST- 2021/04/17 00:00 [received]
PHST- 2021/06/11 00:00 [accepted]
PHST- 2021/07/23 06:52 [entrez]
PHST- 2021/07/24 06:00 [pubmed]
PHST- 2021/07/24 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fvets.2021.696703 [doi]
PST - epublish
SO  - Front Vet Sci. 2021 Jul 6;8:696703. doi: 10.3389/fvets.2021.696703. eCollection 
      2021.