PMID- 3429612
OWN - NLM
STAT- MEDLINE
DCOM- 19880321
LR  - 20131121
IS  - 0270-4145 (Print)
IS  - 0270-4145 (Linking)
VI  - 7
IP  - 4
DP  - 1987
TI  - Hydrocortisone-induced embryotoxicity and embryonic drug disposition in H-2 
      congenic mice.
PG  - 341-56
AB  - Congenic mouse strains C57BL/10Sn (B10) and B10.A/SgSn (B10A), genetically 
      different only in the region of the H-2 complex, were compared for sensitivity to 
      hydrocortisone-induced embryotoxicity and embryonic drug disposition. Pregnant 
      B10A mice dosed intramuscularly with 0, 100, 150, and 200 mg hydrocortisone/kg 
      body weight and B10 mice injected with 0, 200, 400, 600, and 800 mg/kg, both on 
      gestational day (GD) 12, were evaluated on GD 18 for reproductive toxicity. The 
      induction of cleft palate demonstrated a linear dose-response by probit analysis: 
      The ED50s were 143.6 mg/kg and 512.0 mg/kg for B10A and B10 mice, respectively. 
      Comparison of fetal weight revealed statistically significant intrauterine growth 
      retardation at all doses administered to B10 mice. However, growth retardation 
      was shown only in the high-dose group in the B10A strain. Embryonic drug 
      concentrations were evaluated by administration of hydrocortisone to mice of both 
      strains on GD 12, at the ED50 for cleft palate production in the B10A strain, 
      with 3H-hydrocortisone (5 muci/mouse) added as a tracer. Maternal serum and 
      embryos were analyzed for steroid content. Disposition and pharmacokinetics of 
      3H-hydrocortisone were similar in both strains, with the majority of serum 
      radioactivity recovered as hydrocortisone and the major radioactive peak in 
      embryos comigrating with cortisone. The results indicate that H-2 haplotype does 
      not influence hydrocortisone-induced cleft palate sensitivity through an 
      alteration of embryonic drug exposure.
FAU - Roberts, L G
AU  - Roberts LG
AD  - California Primate Research Center, University of California, Davis 95616.
FAU - Hendrickx, A G
AU  - Hendrickx AG
LA  - eng
GR  - RR 00169/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Denmark
TA  - J Craniofac Genet Dev Biol
JT  - Journal of craniofacial genetics and developmental biology
JID - 8109845
RN  - 0 (H-2 Antigens)
RN  - 0 (Teratogens)
RN  - WI4X0X7BPJ (Hydrocortisone)
SB  - IM
MH  - Animals
MH  - Body Weight/drug effects
MH  - Cleft Palate/chemically induced
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Fetus/*drug effects/metabolism
MH  - H-2 Antigens/*genetics
MH  - Hydrocortisone/pharmacokinetics/*toxicity
MH  - Maternal-Fetal Exchange
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred Strains
MH  - Pregnancy
MH  - *Teratogens/pharmacokinetics
EDAT- 1987/01/01 00:00
MHDA- 1987/01/01 00:01
CRDT- 1987/01/01 00:00
PHST- 1987/01/01 00:00 [pubmed]
PHST- 1987/01/01 00:01 [medline]
PHST- 1987/01/01 00:00 [entrez]
PST - ppublish
SO  - J Craniofac Genet Dev Biol. 1987;7(4):341-56.