PMID- 34296991
OWN - NLM
STAT- MEDLINE
DCOM- 20210728
LR  - 20210728
IS  - 1466-5034 (Electronic)
IS  - 1466-5026 (Linking)
VI  - 71
IP  - 7
DP  - 2021 Jul
TI  - Flavobacterium muglaense sp. nov. isolated from internal organs of apparently 
      healthy rainbow trout.
LID - 10.1099/ijsem.0.004903 [doi]
AB  - Two yellow-pigmented isolates, F-60(T) and F-392, were isolated from the internal 
      organs of an apparently healthy rainbow trout (Oncorhynchus mykiss). The strains 
      were identified as members of the genus Flavobacterium based on the results of 
      16S rRNA gene sequence analysis. Strains F-60(T) and F-392 had the highest 16S 
      rRNA gene sequence identity level of 97.4 % to the type strain of Flavobacterium 
      crassostreae LPB0076(T). A polyphasic taxonomic approach including phenotypic, 
      chemotaxonomic and genomic characterization was employed to ascertain the 
      taxonomic position of the strains within the genus Flavobacterium. Digital 
      DNA-DNA hybridization (dDDH) and average nucleotide identity based on blast 
      (ANIb) values for strains F-60(T) and F-392 were calculated as 100 %. However, 
      dDDH and ANI analyses between the strains and their close neighbours confirmed 
      that both strains represent a novel species in the genus Flavobacterium. The 
      strains shared the highest dDDH and ANIb levels of 23.3 and 77.9%, respectively, 
      with the type strain of Flavobacterium frigidarium DSM 17623(T) while those 
      values for F. crassostreae LPB0076(T) were obtained as 21.4-21.5 % and 76.3 %. 
      The DNA G+C content of the strains was 34.5 mol%. Chemotaxonomic and phylogenomic 
      analyses of these isolates confirmed that both strains are representatives of a 
      novel species for which the name Flavobacterium muglaense sp. nov. is proposed, 
      with F-60(T) as the type strain (=JCM 34196(T)=KCTC 82256(T)).
FAU - Duman, Muhammed
AU  - Duman M
AD  - Department of Aquatic Animal Diseases, Faculty of Veterinary Medicine, Bursa 
      Uludag University, Bursa 16059, Turkey.
FAU - Ay, Hilal
AU  - Ay H
AD  - Department of Molecular Biology and Genetics, Faculty of Science and Arts, 
      Ondokuz Mayis University, Samsun 55139, Turkey.
FAU - Altun, Soner
AU  - Altun S
AD  - Department of Aquatic Animal Diseases, Faculty of Veterinary Medicine, Bursa 
      Uludag University, Bursa 16059, Turkey.
FAU - Sahin, Nevzat
AU  - Sahin N
AD  - Department of Molecular Biology and Genetics, Faculty of Science and Arts, 
      Ondokuz Mayis University, Samsun 55139, Turkey.
FAU - Saticioglu, Izzet Burcin
AU  - Saticioglu IB
AD  - Department of Aquatic Animal Diseases, Faculty of Veterinary Medicine, Erciyes 
      University, 38280, Kayseri, Turkey.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Int J Syst Evol Microbiol
JT  - International journal of systematic and evolutionary microbiology
JID - 100899600
RN  - 0 (DNA, Bacterial)
RN  - 0 (Fatty Acids)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Bacterial Typing Techniques
MH  - Base Composition
MH  - DNA, Bacterial/genetics
MH  - Fatty Acids/chemistry
MH  - Flavobacterium/*classification/isolation & purification
MH  - Nucleic Acid Hybridization
MH  - Oncorhynchus mykiss/*microbiology
MH  - *Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
OTO - NOTNLM
OT  - Aquaculture
OT  - Flavobacterium
OT  - genome analysis
OT  - rainbow trout
EDAT- 2021/07/24 06:00
MHDA- 2021/07/29 06:00
CRDT- 2021/07/23 12:15
PHST- 2021/07/23 12:15 [entrez]
PHST- 2021/07/24 06:00 [pubmed]
PHST- 2021/07/29 06:00 [medline]
AID - 10.1099/ijsem.0.004903 [doi]
PST - ppublish
SO  - Int J Syst Evol Microbiol. 2021 Jul;71(7). doi: 10.1099/ijsem.0.004903.