PMID- 34298903 OWN - NLM STAT- MEDLINE DCOM- 20210805 LR - 20210805 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 22 IP - 14 DP - 2021 Jul 6 TI - Key Regulators of Sucrose Metabolism Identified through Comprehensive Comparative Transcriptome Analysis in Peanuts. LID - 10.3390/ijms22147266 [doi] LID - 7266 AB - Sucrose content is a crucial indicator of quality and flavor in peanut seed, and there is a lack of clarity on the molecular basis of sucrose metabolism in peanut seed. In this context, we performed a comprehensive comparative transcriptome study on the samples collected at seven seed development stages between a high-sucrose content variety (ICG 12625) and a low-sucrose content variety (Zhonghua 10). The transcriptome analysis identified a total of 8334 genes exhibiting significantly different abundances between the high- and low-sucrose varieties. We identified 28 differentially expressed genes (DEGs) involved in sucrose metabolism in peanut and 12 of these encoded sugars will eventually be exported transporters (SWEETs). The remaining 16 genes encoded enzymes, such as cell wall invertase (CWIN), vacuolar invertase (VIN), cytoplasmic invertase (CIN), cytosolic fructose-bisphosphate aldolase (FBA), cytosolic fructose-1,6-bisphosphate phosphatase (FBP), sucrose synthase (SUS), cytosolic phosphoglucose isomerase (PGI), hexokinase (HK), and sucrose-phosphate phosphatase (SPP). The weighted gene co-expression network analysis (WGCNA) identified seven genes encoding key enzymes (CIN, FBA, FBP, HK, and SPP), three SWEET genes, and 90 transcription factors (TFs) showing a high correlation with sucrose content. Furthermore, upon validation, six of these genes were successfully verified as exhibiting higher expression in high-sucrose recombinant inbred lines (RILs). Our study suggested the key roles of the high expression of SWEETs and enzymes in sucrose synthesis making the genotype ICG 12625 sucrose-rich. This study also provided insights into the molecular basis of sucrose metabolism during seed development and facilitated exploring key candidate genes and molecular breeding for sucrose content in peanuts. FAU - Li, Weitao AU - Li W AUID- ORCID: 0000-0003-3143-9043 AD - Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China. FAU - Huang, Li AU - Huang L AD - Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China. FAU - Liu, Nian AU - Liu N AD - Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China. FAU - Pandey, Manish K AU - Pandey MK AUID- ORCID: 0000-0002-4101-6530 AD - Center of Excellence in Genomics & Systems Biology, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad 502324, India. FAU - Chen, Yuning AU - Chen Y AD - Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China. FAU - Cheng, Liangqiang AU - Cheng L AD - Oil Research Institute of Guizhou Province, Guizhou Academy of Agricultural Science, Guiyang 550006, China. FAU - Guo, Jianbin AU - Guo J AD - Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China. FAU - Yu, Bolun AU - Yu B AD - Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China. FAU - Luo, Huaiyong AU - Luo H AD - Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China. FAU - Zhou, Xiaojing AU - Zhou X AD - Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China. FAU - Huai, Dongxin AU - Huai D AD - Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China. FAU - Chen, Weigang AU - Chen W AD - Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China. FAU - Yan, Liying AU - Yan L AD - Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China. FAU - Wang, Xin AU - Wang X AD - Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China. FAU - Lei, Yong AU - Lei Y AD - Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China. FAU - Varshney, Rajeev K AU - Varshney RK AUID- ORCID: 0000-0002-4562-9131 AD - Center of Excellence in Genomics & Systems Biology, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad 502324, India. AD - State Agricultural Biotechnology Centre, Centre for Crop and Food Innovation, Murdoch University, Murdoch 6150, Australia. FAU - Liao, Boshou AU - Liao B AD - Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China. FAU - Jiang, Huifang AU - Jiang H AD - Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China. LA - eng GR - CARS-13/National Peanut Industry Technology System Construction/ GR - 2020NWB033/National Program for Crop Germplasm Protection of China/ GR - NCGRC-2020-036/National Crop Germplasm Resources Center/ GR - KF2020008/Open Project of Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, P.R. China/ PT - Journal Article DEP - 20210706 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 57-50-1 (Sucrose) RN - EC 2.4.1.- (Glucosyltransferases) RN - EC 2.4.1.13 (sucrose synthase) RN - EC 3.2.1.26 (beta-Fructofuranosidase) SB - IM MH - Arachis/*genetics/*metabolism MH - Carbohydrate Metabolism/genetics MH - Cell Wall/genetics/metabolism MH - Gene Expression Profiling/methods MH - Gene Expression Regulation, Plant/genetics MH - Glucosyltransferases/genetics/metabolism MH - Seeds/genetics/metabolism MH - Sucrose/*metabolism MH - Transcriptome/*genetics MH - beta-Fructofuranosidase/genetics/metabolism PMC - PMC8306169 OTO - NOTNLM OT - RNA-based sequencing (RNA-seq) OT - development OT - peanut seed OT - sucrose metabolism OT - transcriptional regulation COIS- The authors declare no conflict of interest. EDAT- 2021/07/25 06:00 MHDA- 2021/08/06 06:00 PMCR- 2021/07/06 CRDT- 2021/07/24 01:01 PHST- 2021/05/20 00:00 [received] PHST- 2021/07/02 00:00 [revised] PHST- 2021/07/03 00:00 [accepted] PHST- 2021/07/24 01:01 [entrez] PHST- 2021/07/25 06:00 [pubmed] PHST- 2021/08/06 06:00 [medline] PHST- 2021/07/06 00:00 [pmc-release] AID - ijms22147266 [pii] AID - ijms-22-07266 [pii] AID - 10.3390/ijms22147266 [doi] PST - epublish SO - Int J Mol Sci. 2021 Jul 6;22(14):7266. doi: 10.3390/ijms22147266.