PMID- 34302699
OWN - NLM
STAT- MEDLINE
DCOM- 20211022
LR  - 20211022
IS  - 1399-3054 (Electronic)
IS  - 0031-9317 (Linking)
VI  - 173
IP  - 3
DP  - 2021 Nov
TI  - Xyloglucan endotransglucosylase/hydrolase genes LcXTH4/7/19 are involved in 
      fruitlet abscission and are activated by LcEIL2/3 in litchi.
PG  - 1136-1146
LID - 10.1111/ppl.13509 [doi]
AB  - Organ abscission in plants requires the hydrolysis of cell wall components, 
      mainly including celluloses, pectins, and xyloglucans. However, how the genes 
      that encode those hydrolytic enzymes are regulated and their function in 
      abscission remains unclear. Previously we revealed that two cellulase genes 
      LcCEL2/8 and two polygalacturonase genes LcPG1/2 were responsible for the 
      degradation of celluloses and pectins, respectively, during fruitlet abscission 
      in litchi. Here, we further identified three xyloglucan 
      endotransglucosylase/hydrolase genes (LcXTH4, LcXTH7, LcXTH19) that are also 
      involved in this process. Nineteen LcXTHs, named LcXTH1-19, were identified in 
      the litchi genome. Transcriptome data and qRT-PCR confirmed that LcXTH4/7/19 were 
      significantly induced at the abscission zone (AZ) during fruitlet abscission in 
      litchi. The GUS reporter driven by each promoter of LcXTH4/7/19 was specifically 
      expressed at the floral abscission zone of Arabidopsis, and importantly ectopic 
      expression of LcXTH19 in Arabidopsis resulted in precocious floral organ 
      abscission. Moreover, electrophoretic mobility shift assay (EMSA) and 
      dual-luciferase reporter analysis showed that the expression of LcXTH4/7/19 could 
      be directly activated by two ETHYLENE INSENSITIVE 3-like (EIL) transcription 
      factors LcEIL2/3. Collectively, we propose that LcXTH4/7/19 are involved in 
      fruitlet abscission, and LcEIL2/3-mediated transcriptional regulation of diverse 
      cell wall hydrolytic genes is responsible for this process in litchi.
CI  - (c) 2021 Scandinavian Plant Physiology Society.
FAU - Ma, Xingshuai
AU  - Ma X
AUID- ORCID: 0000-0003-4795-9596
AD  - State Key Laboratory for Conservation and Utilization of Subtropical 
      Agro-Bioresources, China Litchi Research Center, South China Agricultural 
      University, Guangzhou, China.
AD  - Guangdong Litchi Engineering Research Center, College of Horticulture, South 
      China Agricultural University, Guangzhou, China.
FAU - Li, Caiqin
AU  - Li C
AD  - State Key Laboratory for Conservation and Utilization of Subtropical 
      Agro-Bioresources, China Litchi Research Center, South China Agricultural 
      University, Guangzhou, China.
AD  - Guangdong Litchi Engineering Research Center, College of Horticulture, South 
      China Agricultural University, Guangzhou, China.
FAU - Yuan, Ye
AU  - Yuan Y
AD  - State Key Laboratory for Conservation and Utilization of Subtropical 
      Agro-Bioresources, China Litchi Research Center, South China Agricultural 
      University, Guangzhou, China.
AD  - Guangdong Litchi Engineering Research Center, College of Horticulture, South 
      China Agricultural University, Guangzhou, China.
FAU - Zhao, Minglei
AU  - Zhao M
AD  - State Key Laboratory for Conservation and Utilization of Subtropical 
      Agro-Bioresources, China Litchi Research Center, South China Agricultural 
      University, Guangzhou, China.
AD  - Guangdong Litchi Engineering Research Center, College of Horticulture, South 
      China Agricultural University, Guangzhou, China.
AD  - Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and 
      Vegetables, College of Horticulture, South China Agricultural University, 
      Guangzhou, China.
FAU - Li, Jianguo
AU  - Li J
AD  - State Key Laboratory for Conservation and Utilization of Subtropical 
      Agro-Bioresources, China Litchi Research Center, South China Agricultural 
      University, Guangzhou, China.
AD  - Guangdong Litchi Engineering Research Center, College of Horticulture, South 
      China Agricultural University, Guangzhou, China.
LA  - eng
GR  - 2019LHPY001/Graduate Student Overseas Study Program from South China Agricultural 
      University/
GR  - 201804020063/Science and Technology Key Project of Guangzhou/
GR  - South China Agricultural University/
GR  - 32072544/National Natural Science Foundation of China/
GR  - 32072514/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20210818
PL  - Denmark
TA  - Physiol Plant
JT  - Physiologia plantarum
JID - 1256322
RN  - 0 (Plant Proteins)
RN  - EC 2.4.- (Glycosyltransferases)
RN  - EC 2.4.1.207 (xyloglucan - xyloglucosyltransferase)
RN  - EC 3.- (Hydrolases)
SB  - IM
MH  - Glycosyltransferases/genetics
MH  - Hydrolases
MH  - *Litchi/genetics
MH  - Plant Proteins/genetics
EDAT- 2021/07/25 06:00
MHDA- 2023/02/25 06:00
CRDT- 2021/07/24 12:13
PHST- 2021/06/23 00:00 [revised]
PHST- 2021/01/26 00:00 [received]
PHST- 2021/07/19 00:00 [accepted]
PHST- 2021/07/25 06:00 [pubmed]
PHST- 2023/02/25 06:00 [medline]
PHST- 2021/07/24 12:13 [entrez]
AID - 10.1111/ppl.13509 [doi]
PST - ppublish
SO  - Physiol Plant. 2021 Nov;173(3):1136-1146. doi: 10.1111/ppl.13509. Epub 2021 Aug 
      18.