PMID- 34304344
OWN - NLM
STAT- MEDLINE
DCOM- 20220208
LR  - 20220208
IS  - 1615-7605 (Electronic)
IS  - 1615-7591 (Linking)
VI  - 44
IP  - 11
DP  - 2021 Nov
TI  - Novel dual-flow perfusion bioreactor for in vitro pre-screening of nanoparticles 
      delivery: design, characterization and testing.
PG  - 2361-2374
LID - 10.1007/s00449-021-02609-4 [doi]
AB  - An advanced dual-flow perfusion bioreactor with a simple and compact design was 
      developed and evaluated as a potential apparatus to reduce the gap between animal 
      testing and drug administration to human subjects in clinical trials. All the 
      experimental tests were carried out using an ad hoc Poly Lactic Acid (PLLA) 
      scaffold synthesized via Thermally Induced Phase Separation (TIPS). The 
      bioreactor shows a tunable radial flow throughout the microporous matrix of the 
      scaffold. The radial perfusion was quantified both with permeability tests and 
      with a mathematical model, applying a combination of Darcy's Theory, Bernoulli's 
      Equation, and Poiseuille's Law. Finally, a diffusion test allowed to investigate 
      the efficacy of the radial flow using Polymeric Fluorescent Nanoparticles (FNPs) 
      mimicking drug/colloidal carriers. These tests confirmed the ability of our 
      bioreactor to create a uniform distribution of particles inside porous matrices. 
      All the findings candidate our system as a potential tool for drug pre-screening 
      testing with a cost and time reduction over animal models.
CI  - (c) 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, 
      part of Springer Nature.
FAU - Lombardo, Maria Elena
AU  - Lombardo ME
AUID- ORCID: 0000-0003-4685-3503
AD  - Dipartimento di Ingegneria, University of Palermo, Viale delle Scienze building 
      8, 90128, Palermo, Italy. mariaelena.lombardo01@unipa.it.
AD  - Centre Energie, Materiaux et Telecommunications, Institut National de la 
      Recherche Scientifique, 1650 Boulevard Lionel-Boulet, Varennes, QC, J3X 1S2, 
      Canada. mariaelena.lombardo01@unipa.it.
FAU - Carfi Pavia, Francesco
AU  - Carfi Pavia F
AD  - Dipartimento di Ingegneria, University of Palermo, Viale delle Scienze building 
      8, 90128, Palermo, Italy.
AD  - Consorzio Universitario di Caltanissetta, Corso Vittorio Emanuele 92, 93100, 
      Caltanissetta, Italy.
FAU - Craparo, Emanuela Fabiola
AU  - Craparo EF
AD  - Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche, 
      University of Palermo, Viale delle Scienze building 16, 90128, Palermo, Italy.
FAU - Capuana, Elisa
AU  - Capuana E
AD  - Dipartimento di Ingegneria, University of Palermo, Viale delle Scienze building 
      8, 90128, Palermo, Italy.
FAU - Cavallaro, Gennara
AU  - Cavallaro G
AD  - Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche, 
      University of Palermo, Viale delle Scienze building 16, 90128, Palermo, Italy.
AD  - ATeN center, University of Palermo, Viale delle Scienze building 18, 90128, 
      Palermo, Italy.
FAU - Brucato, Valerio
AU  - Brucato V
AD  - Dipartimento di Ingegneria, University of Palermo, Viale delle Scienze building 
      8, 90128, Palermo, Italy.
FAU - La Carrubba, Vincenzo
AU  - La Carrubba V
AD  - Dipartimento di Ingegneria, University of Palermo, Viale delle Scienze building 
      8, 90128, Palermo, Italy.
AD  - ATeN center, University of Palermo, Viale delle Scienze building 18, 90128, 
      Palermo, Italy.
LA  - eng
PT  - Journal Article
DEP - 20210725
PL  - Germany
TA  - Bioprocess Biosyst Eng
JT  - Bioprocess and biosystems engineering
JID - 101088505
RN  - 0 (Biocompatible Materials)
RN  - 0 (Drug Carriers)
RN  - 0 (Polymers)
SB  - IM
MH  - Animals
MH  - Biocompatible Materials
MH  - *Bioreactors
MH  - Drug Carriers
MH  - Humans
MH  - In Vitro Techniques
MH  - Microscopy, Electron, Scanning
MH  - Nanoparticles/*administration & dosage
MH  - Polymers/chemistry
MH  - Tissue Engineering
OTO - NOTNLM
OT  - 3D Scaffold
OT  - Dual-flow perfusion bioreactor
OT  - Fluid dynamics
OT  - Polymeric fluorescent nanoparticles
OT  - Tunable radial flow
EDAT- 2021/07/26 06:00
MHDA- 2022/02/09 06:00
CRDT- 2021/07/25 20:55
PHST- 2021/04/20 00:00 [received]
PHST- 2021/06/26 00:00 [accepted]
PHST- 2021/07/26 06:00 [pubmed]
PHST- 2022/02/09 06:00 [medline]
PHST- 2021/07/25 20:55 [entrez]
AID - 10.1007/s00449-021-02609-4 [pii]
AID - 10.1007/s00449-021-02609-4 [doi]
PST - ppublish
SO  - Bioprocess Biosyst Eng. 2021 Nov;44(11):2361-2374. doi: 
      10.1007/s00449-021-02609-4. Epub 2021 Jul 25.