PMID- 34307535
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210727
IS  - 2297-1769 (Print)
IS  - 2297-1769 (Electronic)
IS  - 2297-1769 (Linking)
VI  - 8
DP  - 2021
TI  - Mycobacterial Infection of Precision-Cut Lung Slices Reveals Type 1 Interferon 
      Pathway Is Locally Induced by Mycobacterium bovis but Not M. tuberculosis in a 
      Cattle Breed.
PG  - 696525
LID - 10.3389/fvets.2021.696525 [doi]
LID - 696525
AB  - Tuberculosis exacts a terrible toll on human and animal health. While 
      Mycobacterium tuberculosis (Mtb) is restricted to humans, Mycobacterium bovis 
      (Mb) is present in a large range of mammalian hosts. In cattle, bovine TB (bTB) 
      is a noticeable disease responsible for important economic losses in developed 
      countries and underestimated zoonosis in the developing world. Early interactions 
      that take place between mycobacteria and the lung tissue early after aerosol 
      infection govern the outcome of the disease. In cattle, these early steps remain 
      poorly characterized. The precision-cut lung slice (PCLS) model preserves the 
      structure and cell diversity of the lung. We developed this model in cattle in 
      order to study the early lung response to mycobacterial infection. In situ 
      imaging of PCLS infected with fluorescent Mb revealed bacilli in the alveolar 
      compartment, in adjacent or inside alveolar macrophages, and in close contact 
      with pneumocytes. We analyzed the global transcriptional lung inflammation 
      signature following infection of PCLS with Mb and Mtb in two French beef breeds: 
      Blonde d'Aquitaine and Charolaise. Whereas, lungs from the Blonde d'Aquitaine 
      produced high levels of mediators of neutrophil and monocyte recruitment in 
      response to infection, such signatures were not observed in the Charolaise in our 
      study. In the Blonde d'Aquitaine lung, whereas the inflammatory response was 
      highly induced by two Mb strains, AF2122 isolated from cattle in the UK and 
      Mb3601 circulating in France, the response against two Mtb strains, H37Rv, the 
      reference laboratory strain, and BTB1558, isolated from zebu in Ethiopia, was 
      very low. Strikingly, the type I interferon pathway was only induced by Mb but 
      not Mtb strains, indicating that this pathway may be involved in mycobacterial 
      virulence and host tropism. Hence, the PCLS model in cattle is a valuable tool to 
      deepen our understanding of early interactions between lung host cells and 
      mycobacteria. It revealed striking differences between cattle breeds and 
      mycobacterial strains. This model could help in deciphering biomarkers of 
      resistance vs. susceptibility to bTB in cattle as such information is still 
      critically needed for bovine genetic selection programs and would greatly help 
      the global effort to eradicate bTB.
CI  - Copyright (c) 2021 Remot, Carreras, Coupe, Doz-Deblauwe, Boschiroli, Browne, 
      Marquant, Descamps, Archer, Aseffa, Germon, Gordon and Winter.
FAU - Remot, Aude
AU  - Remot A
AD  - INRAE, Universite de Tours, Nouzilly, France.
FAU - Carreras, Florence
AU  - Carreras F
AD  - INRAE, Universite de Tours, Nouzilly, France.
FAU - Coupe, Anthony
AU  - Coupe A
AD  - INRAE, Universite de Tours, Nouzilly, France.
FAU - Doz-Deblauwe, Emilie
AU  - Doz-Deblauwe E
AD  - INRAE, Universite de Tours, Nouzilly, France.
FAU - Boschiroli, Maria L
AU  - Boschiroli ML
AD  - Paris-Est University, National Reference Laboratory for Tuberculosis, Animal 
      Health Laboratory, Anses, Maisons-Alfort, France.
FAU - Browne, John A
AU  - Browne JA
AD  - UCD School of Agriculture and Food Science, University College Dublin, Dublin, 
      Ireland.
FAU - Marquant, Quentin
AU  - Marquant Q
AD  - INRAE, Universite Paris-Saclay, UVSQ, Jouy-en-Josas, France.
FAU - Descamps, Delphyne
AU  - Descamps D
AD  - INRAE, Universite Paris-Saclay, UVSQ, Jouy-en-Josas, France.
FAU - Archer, Fabienne
AU  - Archer F
AD  - INRAE, UMR754, Viral Infections and Comparative Pathology, IVPC, Univ Lyon, 
      Universite Claude Bernard Lyon 1, EPHE, Lyon, France.
FAU - Aseffa, Abraham
AU  - Aseffa A
AD  - Armauer Hansen Research Institute, Addis Ababa, Ethiopia.
FAU - Germon, Pierre
AU  - Germon P
AD  - INRAE, Universite de Tours, Nouzilly, France.
FAU - Gordon, Stephen V
AU  - Gordon SV
AD  - UCD School of Veterinary Medicine and UCD Conway Institute, University College 
      Dublin, Dublin, Ireland.
FAU - Winter, Nathalie
AU  - Winter N
AD  - INRAE, Universite de Tours, Nouzilly, France.
LA  - eng
PT  - Journal Article
DEP - 20210709
PL  - Switzerland
TA  - Front Vet Sci
JT  - Frontiers in veterinary science
JID - 101666658
PMC - PMC8299756
OTO - NOTNLM
OT  - Mycobacterium bovis
OT  - alveolar macrophages
OT  - cattle
OT  - ex vivo
OT  - precision cut lung slices
OT  - type I interferon
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest. The handling editor declared a past collaboration with one 
      of the authors SG.
EDAT- 2021/07/27 06:00
MHDA- 2021/07/27 06:01
PMCR- 2021/01/01
CRDT- 2021/07/26 06:39
PHST- 2021/04/16 00:00 [received]
PHST- 2021/06/02 00:00 [accepted]
PHST- 2021/07/26 06:39 [entrez]
PHST- 2021/07/27 06:00 [pubmed]
PHST- 2021/07/27 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fvets.2021.696525 [doi]
PST - epublish
SO  - Front Vet Sci. 2021 Jul 9;8:696525. doi: 10.3389/fvets.2021.696525. eCollection 
      2021.