PMID- 34308380
OWN - NLM
STAT- MEDLINE
DCOM- 20220321
LR  - 20240402
IS  - 2666-1667 (Electronic)
IS  - 2666-1667 (Linking)
VI  - 2
IP  - 3
DP  - 2021 Sep 17
TI  - Microglia replacement by microglia transplantation (Mr MT) in the adult mouse 
      brain.
PG  - 100665
LID - 10.1016/j.xpro.2021.100665 [doi]
LID - 100665
AB  - Mutations in microglia may cause brain disorders. Replacement of dysfunctional 
      microglia by allogeneic wild-type microglia from bone marrow transplantation (Mr 
      BMT) or peripheral blood can correct the gene deficiency at the brain-wide scale 
      but cannot achieve precise replacement at specific brain regions. Here, we 
      introduce a strategy with potential clinical relevance-microglia replacement by 
      microglia transplantation (Mr MT), combining tamoxifen-induced ablation of Mr BMT 
      cells and intracranial injection of microglia to mouse brain, to achieve 
      region-sepcific microglia replacement. The original abbreviation of this 
      microglia replacement strategy is mrMT. We hereby change the name to Mr MT. For 
      complete details on the use and execution of this protocol, please refer to Xu 
      et al. (2020).
CI  - (c) 2021 The Author(s).
FAU - Xu, Zhen
AU  - Xu Z
AD  - Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 
      518055, China.
FAU - Peng, Bo
AU  - Peng B
AD  - Department of Neurosurgery, Huashan Hospital, Institute for Translational Brain 
      Research, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for 
      Brain Science, Fudan University, Shanghai 200032, China.
FAU - Rao, Yanxia
AU  - Rao Y
AD  - Shanghai Key Laboratory of Psychotic Disorders, Shanghai Mental Health Center, 
      Shanghai Jiao Tong University School of Medicine, Shanghai 201108, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210709
PL  - United States
TA  - STAR Protoc
JT  - STAR protocols
JID - 101769501
RN  - 0 (CX3C Chemokine Receptor 1)
RN  - 0 (Cx3cr1 protein, mouse)
RN  - 094ZI81Y45 (Tamoxifen)
RN  - 147336-22-9 (Green Fluorescent Proteins)
MH  - Animals
MH  - Bone Marrow Transplantation/methods
MH  - Brain/*cytology/physiology
MH  - CX3C Chemokine Receptor 1/genetics
MH  - Female
MH  - Green Fluorescent Proteins/genetics
MH  - Male
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Microglia/drug effects/*transplantation
MH  - Tamoxifen/pharmacology
MH  - Tissue Transplantation/instrumentation/*methods
MH  - Transplants
MH  - Mice
PMC - PMC8283144
OTO - NOTNLM
OT  - Cell Biology
OT  - Immunology
OT  - Microscopy
OT  - Model Organisms
OT  - Neuroscience
OT  - Stem Cells
COIS- The authors declare no competing interests.
EDAT- 2021/07/27 06:00
MHDA- 2021/07/27 06:01
PMCR- 2021/07/09
CRDT- 2021/07/26 06:50
PHST- 2021/07/26 06:50 [entrez]
PHST- 2021/07/27 06:00 [pubmed]
PHST- 2021/07/27 06:01 [medline]
PHST- 2021/07/09 00:00 [pmc-release]
AID - S2666-1667(21)00372-5 [pii]
AID - 100665 [pii]
AID - 10.1016/j.xpro.2021.100665 [doi]
PST - epublish
SO  - STAR Protoc. 2021 Jul 9;2(3):100665. doi: 10.1016/j.xpro.2021.100665. eCollection 
      2021 Sep 17.