PMID- 34309053
OWN - NLM
STAT- MEDLINE
DCOM- 20210923
LR  - 20210923
IS  - 1365-2052 (Electronic)
IS  - 0268-9146 (Linking)
VI  - 52
IP  - 5
DP  - 2021 Oct
TI  - Alternative polyadenylation events differ dramatically between Tongcheng and 
      Large White pigs in response to PRRSV infection.
PG  - 744-748
LID - 10.1111/age.13125 [doi]
AB  - Alternative polyadenylation (APA) is a widespread post-transcriptional regulation 
      mechanism that increases the biological complexity of transcriptome and proteome. 
      However, it is unclear whether APA regulation plays a role in genetic resistance 
      to porcine reproductive and respiratory syndrome virus (PRRSV). Here, we reported 
      genome-wide APA regulation of porcine alveolar macrophages in PRRSV-resistant 
      Tongcheng (TC) pigs and PRRSV-susceptible Large White (LW) pigs upon PRRSV 
      infection. Using 3' mRNA sequencing strategy, we detected 75 981 high-quality APA 
      sites in porcine alveolar macrophages of TC and LW pigs. Furthermore, 1202 and 
      1089 differentially expressed APA sites, as well as 79 and 117 untranslated 
      region-APA switching genes were identified in TC pigs and LW pigs upon PRRSV 
      infection respectively. The APA events in TC pigs and LW pigs were involved in 
      different biological pathways, while APA events in TC pigs are directly 
      associated with the immune response to PRRSV infection. In addition, we 
      identified genetic variations affecting polyadenylation signal between TC pigs 
      and LW pigs. These findings would provide helpful information on APA regulation 
      for further understanding of genetic resistance to PRRSV.
CI  - (c) 2021 Stichting International Foundation for Animal Genetics.
FAU - Meng, X
AU  - Meng X
AD  - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of 
      Ministry of Education, College of Animal Science and Technology, Huazhong 
      Agricultural University, Wuhan, 430070, China.
FAU - Kuang, K
AU  - Kuang K
AD  - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of 
      Ministry of Education, College of Animal Science and Technology, Huazhong 
      Agricultural University, Wuhan, 430070, China.
FAU - Zhang, Y
AU  - Zhang Y
AD  - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of 
      Ministry of Education, College of Animal Science and Technology, Huazhong 
      Agricultural University, Wuhan, 430070, China.
FAU - Guan, K
AU  - Guan K
AD  - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of 
      Ministry of Education, College of Animal Science and Technology, Huazhong 
      Agricultural University, Wuhan, 430070, China.
FAU - Liu, B
AU  - Liu B
AUID- ORCID: 0000-0002-3539-6020
AD  - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of 
      Ministry of Education, College of Animal Science and Technology, Huazhong 
      Agricultural University, Wuhan, 430070, China.
AD  - The Engineering Technology Research Center of Hubei Province Local Pig Breed 
      Improvement, Wuhan, 430070, China.
FAU - Zhou, X
AU  - Zhou X
AUID- ORCID: 0000-0002-5727-6783
AD  - Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of 
      Ministry of Education, College of Animal Science and Technology, Huazhong 
      Agricultural University, Wuhan, 430070, China.
AD  - The Engineering Technology Research Center of Hubei Province Local Pig Breed 
      Improvement, Wuhan, 430070, China.
LA  - eng
GR  - 319300104 and 31802040/National Natural Science Foundation of China/
GR  - 2019CFB454/Hubei Provincial Natural Science Foundation of China/
GR  - 2662018QD004/Fundamental Research Funds for the Central Universities/
PT  - Journal Article
DEP - 20210726
PL  - England
TA  - Anim Genet
JT  - Animal genetics
JID - 8605704
SB  - IM
MH  - Animals
MH  - Breeding
MH  - Gene Expression Regulation
MH  - Macrophages, Alveolar/metabolism
MH  - *Polyadenylation
MH  - Porcine Reproductive and Respiratory Syndrome/*genetics
MH  - Porcine respiratory and reproductive syndrome virus
MH  - Sus scrofa/*genetics/virology
MH  - Swine
OTO - NOTNLM
OT  - 3' mRNA-Seq
OT  - Large White pigs
OT  - Tongcheng pigs
OT  - alternative polyadenylation
OT  - porcine reproductive and respiratory syndrome virus
EDAT- 2021/07/27 06:00
MHDA- 2021/09/24 06:00
CRDT- 2021/07/26 09:00
PHST- 2021/07/05 00:00 [accepted]
PHST- 2021/07/27 06:00 [pubmed]
PHST- 2021/09/24 06:00 [medline]
PHST- 2021/07/26 09:00 [entrez]
AID - 10.1111/age.13125 [doi]
PST - ppublish
SO  - Anim Genet. 2021 Oct;52(5):744-748. doi: 10.1111/age.13125. Epub 2021 Jul 26.