PMID- 34309459
OWN - NLM
STAT- MEDLINE
DCOM- 20220228
LR  - 20221207
IS  - 1532-432X (Electronic)
IS  - 0363-0269 (Linking)
VI  - 45
IP  - 3
DP  - 2021 May
TI  - Hb A(2)-Calderdale [delta2(NA2)His-->Asn; HBD: c.7C>A] and Misdiagnosis of Type 2 
      Diabetes Mellitus Due to Interference with Hb A(1c) When Using Cation Exchange 
      High Performance Liquid Chromatography: A Case Report.
PG  - 207-209
LID - 10.1080/03630269.2021.1954945 [doi]
AB  - The Hb A(2)-Calderdale variant [delta2(NA2)His-->Asn, HBD: c.7C>A] was described as a 
      novel variant in 2014. However, a high performance liquid chromatography (HPLC) 
      peak was never identified indicating that this fraction could be 'hiding' 
      somewhere else in the chromatogram. In this case report, we present evidence that 
      the physiochemical properties of Hb A(2)-Calderdale resemble those of Hb A(1c), 
      resulting in a coelution in variant mode on the Tosoh G8 but not the Tosoh G11. 
      This coelution results in an overestimation of Hb A(1c) and can potentially cause 
      misdiagnosis of type 2 diabetes mellitus (T2DM).
FAU - Stangerup, Ida
AU  - Stangerup I
AUID- ORCID: 0000-0003-3192-150X
AD  - Department of Clinical Biochemistry, Bispebjerg and Frederiksberg Hospital, 
      Copenhagen, Denmark.
FAU - Petersen, Jesper
AU  - Petersen J
AUID- ORCID: 0000-0001-6116-5114
AD  - Department of Hematology, Danish Center for Hemoglobinopathies, Copenhagen, 
      Denmark.
FAU - Glenthoj, Andreas
AU  - Glenthoj A
AUID- ORCID: 0000-0003-2082-0738
AD  - Department of Hematology, Danish Center for Hemoglobinopathies, Copenhagen, 
      Denmark.
LA  - eng
PT  - Case Reports
PT  - Journal Article
DEP - 20210726
PL  - England
TA  - Hemoglobin
JT  - Hemoglobin
JID - 7705865
RN  - 0 (Cations)
RN  - 0 (Glycated Hemoglobin A)
RN  - 0 (Hemoglobins, Abnormal)
SB  - IM
MH  - Cations
MH  - Chromatography, High Pressure Liquid
MH  - *Diabetes Mellitus, Type 2/diagnosis
MH  - *Diagnostic Errors
MH  - Glycated Hemoglobin/analysis
MH  - *Hemoglobins, Abnormal/genetics
MH  - Humans
OTO - NOTNLM
OT  - Hb A1c
OT  - Hb A2-Calderdale
OT  - high performance liquid chromatography (HPLC)
OT  - delta-Globin gene
EDAT- 2021/07/27 06:00
MHDA- 2022/03/01 06:00
CRDT- 2021/07/26 12:18
PHST- 2021/07/27 06:00 [pubmed]
PHST- 2022/03/01 06:00 [medline]
PHST- 2021/07/26 12:18 [entrez]
AID - 10.1080/03630269.2021.1954945 [doi]
PST - ppublish
SO  - Hemoglobin. 2021 May;45(3):207-209. doi: 10.1080/03630269.2021.1954945. Epub 2021 
      Jul 26.