PMID- 34311833
OWN - NLM
STAT- MEDLINE
DCOM- 20210728
LR  - 20210728
IS  - 1000-8020 (Print)
IS  - 1000-8020 (Linking)
VI  - 50
IP  - 4
DP  - 2021 Jul
TI  - [Determination of vitamin A, vitamin E isomers and cholesterol in foods by 
      reversed-phase high-performance liquid chromatography].
PG  - 615-619
LID - 10.19813/j.cnki.weishengyanjiu.2021.04.013 [doi]
AB  - OBJECTIVE: To establish a method for simultaneous analysis of vitamin A, three 
      vitamin E isomers and cholesterol in meat products by reversed-phase 
      high-performance liquid chromatography. This method is used to determine the 
      contents of the corresponding nutrients in several meat products. METHODS: The 
      sample was pretreated with saponification and liquid-liquid extraction, then 
      separated on Waters Symmetry C_(18) column(4.6 mmx250 mm, 5 mum). The mobile phase 
      consisted of methanol(98%) and water(2%). The analytes were detected by 
      photo-diode array(PDA)detector. 325 nm, 294 nm, and 210 nm were selected as the 
      characteristic absorption wavelengths of vitamin A, vitamin E, and cholesterol, 
      respectively by scanning in the range of 190-350 nm in 3 D mode. Benzo[e]pyrene 
      was internal standard for vitamin A and E. Cholesterol was quantified by external 
      standard curve method. RESULTS: The concentration of vitamin A, three vitamin E 
      isomers and cholesterol showed good linearity in the range of 0.18-9.00 
      mug/mL、0.76-5.2 mug/mL and 0.11-5.50 mg/mL, respectively, with the correlation 
      coefficients greater than 0.995.The limits of detections(LODs) were 5、20 and 330 
      mug/100 g and the limits of quantification(LOQs) for vitamin A, three vitamin E 
      isomers and cholesterol were 15、60 and 990 mug/100 g, respectively. The recoveries 
      at three levels in the matrix were 86.3%-105.6%, and the relative standard 
      deviations(RSDs) were all less than 7.0%(n=6). CONCLUSION: The method is 
      sensitive, accurate and suitable for analysis of vitamin A, three vitamin E 
      isomers and cholesterol in meat products.
FAU - Yin, Danyang
AU  - Yin D
AD  - Center for Disease Control and Prevention of Shaanxi Province, Xi'an 710054, 
      China.
FAU - Hu, Jiawei
AU  - Hu J
AD  - Center for Disease Control and Prevention of Shaanxi Province, Xi'an 710054, 
      China.
FAU - Zhao, Di
AU  - Zhao D
AD  - Center for Disease Control and Prevention of Shaanxi Province, Xi'an 710054, 
      China.
FAU - Zhao, Ying
AU  - Zhao Y
AD  - Center for Disease Control and Prevention of Shaanxi Province, Xi'an 710054, 
      China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Wei Sheng Yan Jiu
JT  - Wei sheng yan jiu = Journal of hygiene research
JID - 9426367
RN  - 11103-57-4 (Vitamin A)
RN  - 1406-18-4 (Vitamin E)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Cholesterol
MH  - Chromatography, High Pressure Liquid
MH  - Liquid-Liquid Extraction
MH  - *Vitamin A
MH  - *Vitamin E
OTO - NOTNLM
OT  - cholesterol
OT  - high-performance liquid chromatography
OT  - meat products
OT  - vitamin A
OT  - vitamin E isomer
EDAT- 2021/07/28 06:00
MHDA- 2021/07/29 06:00
CRDT- 2021/07/27 05:47
PHST- 2021/07/27 05:47 [entrez]
PHST- 2021/07/28 06:00 [pubmed]
PHST- 2021/07/29 06:00 [medline]
AID - 10.19813/j.cnki.weishengyanjiu.2021.04.013 [doi]
PST - ppublish
SO  - Wei Sheng Yan Jiu. 2021 Jul;50(4):615-619. doi: 
      10.19813/j.cnki.weishengyanjiu.2021.04.013.