PMID- 34313929
OWN - NLM
STAT- MEDLINE
DCOM- 20211026
LR  - 20220531
IS  - 1573-2630 (Electronic)
IS  - 0165-5701 (Linking)
VI  - 41
IP  - 11
DP  - 2021 Nov
TI  - MicroRNA-93-5p participates in type 2 diabetic retinopathy through targeting 
      Sirt1.
PG  - 3837-3848
LID - 10.1007/s10792-021-01953-4 [doi]
AB  - OBJECTIVE: To investigate the role of miR-93-5p in rats with type 2 diabetic 
      retinopathy (DR) through targeting Sirt1. METHODS: The targeting correlation 
      between miR-93-5p and Sirt1 was validated by dual-luciferase reporter gene assay. 
      Type 2 diabetes mellitus (T2DM) rat models were received intravitreal injection 
      of antagomir NC (negative control), miR-93-5p antagomir, miR-93-5p agomir and/or 
      recombinant Sirt1, followed by observation of pathological changes in retina via 
      HE staining. Besides, retinal vascular permeability was determined by fluorescein 
      isothiocyanate-bovine serum albumin (FITC-BSA), while the retinal vasculature was 
      observed through retinal trypsin digestion. Expression of miR-93-5p and Sirt1 was 
      measured by qRT-PCR and Western blotting, while the levels of VEGF, 
      proinflammatory cytokines and anti-oxidative indicators were determined using 
      corresponding kits. RESULTS: MiR-93-5p could target Sirt1 as analyzed by the 
      luciferase reporter gene assay. Rats in the T2DM group presented the 
      up-regulation of miR-93-5p and down-regulation of Sirt1 in the retina, and 
      miR-93-5p inhibition could up-regulate Sirt1 expression in the T2DM rats. 
      Recombinant Sirt1 decreased retinal vascular permeability and acellular 
      capillaries with improved pathological changes in retina from T2DM rats, which 
      was abolished by miR-93-5p agomir. Moreover, miR-93-5p inhibition or Sirt1 
      overexpression decreased the levels of VEGF and proinflammatory cytokines while 
      enhancing the activity of anti-oxidative indicators. However, indicators above 
      had no significant differences between T2DM group and T2DM + agomir + Sirt1 
      group. CONCLUSION: MiR-93-5p, via targeting Sirt1, could affect the vascular 
      permeability and acellular capillaries and mitigate the inflammation and 
      oxidative stress in the retinas, which may play a critical role in DR.
CI  - (c) 2021. The Author(s), under exclusive licence to Springer Nature B.V.
FAU - Wang, Hui
AU  - Wang H
AD  - Department of Ophthalmology, Shijiazhuang People's Hospital, No. 365, Jianhua 
      South Street, Yuhua District, Shijiazhuang, 050030, Hebei Province, China.
FAU - Su, Xian
AU  - Su X
AD  - Department of Ophthalmology, Shijiazhuang People's Hospital, No. 365, Jianhua 
      South Street, Yuhua District, Shijiazhuang, 050030, Hebei Province, China.
FAU - Zhang, Qian-Qian
AU  - Zhang QQ
AD  - Department of Ophthalmology, Shijiazhuang People's Hospital, No. 365, Jianhua 
      South Street, Yuhua District, Shijiazhuang, 050030, Hebei Province, China.
FAU - Zhang, Ying-Ying
AU  - Zhang YY
AD  - Department of Ophthalmology, Shijiazhuang People's Hospital, No. 365, Jianhua 
      South Street, Yuhua District, Shijiazhuang, 050030, Hebei Province, China.
FAU - Chu, Zhan-Ya
AU  - Chu ZY
AD  - Department of Ophthalmology, Shijiazhuang People's Hospital, No. 365, Jianhua 
      South Street, Yuhua District, Shijiazhuang, 050030, Hebei Province, China.
FAU - Zhang, Jin-Ling
AU  - Zhang JL
AD  - Department of Ophthalmology, Shijiazhuang People's Hospital, No. 365, Jianhua 
      South Street, Yuhua District, Shijiazhuang, 050030, Hebei Province, China.
FAU - Ren, Qian
AU  - Ren Q
AD  - Department of Ophthalmology, Shijiazhuang People's Hospital, No. 365, Jianhua 
      South Street, Yuhua District, Shijiazhuang, 050030, Hebei Province, China. 
      17603119856@163.com.
LA  - eng
PT  - Journal Article
DEP - 20210727
PL  - Netherlands
TA  - Int Ophthalmol
JT  - International ophthalmology
JID - 7904294
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn93 microRNA, rat)
RN  - EC 3.5.1.- (Sirt1 protein, rat)
RN  - EC 3.5.1.- (Sirtuin 1)
SB  - IM
MH  - Animals
MH  - *Diabetes Mellitus, Type 2/complications/genetics
MH  - *Diabetic Retinopathy/genetics
MH  - *MicroRNAs/genetics
MH  - Rats
MH  - Sirtuin 1/genetics/metabolism
MH  - Up-Regulation
OTO - NOTNLM
OT  - Diabetic retinopathy
OT  - Mir-93-5p
OT  - Sirt1
OT  - T2DM
EDAT- 2021/07/28 06:00
MHDA- 2021/10/27 06:00
CRDT- 2021/07/27 12:33
PHST- 2021/02/07 00:00 [received]
PHST- 2021/07/09 00:00 [accepted]
PHST- 2021/07/28 06:00 [pubmed]
PHST- 2021/10/27 06:00 [medline]
PHST- 2021/07/27 12:33 [entrez]
AID - 10.1007/s10792-021-01953-4 [pii]
AID - 10.1007/s10792-021-01953-4 [doi]
PST - ppublish
SO  - Int Ophthalmol. 2021 Nov;41(11):3837-3848. doi: 10.1007/s10792-021-01953-4. Epub 
      2021 Jul 27.