PMID- 34314522
OWN - NLM
STAT- MEDLINE
DCOM- 20220224
LR  - 20220224
IS  - 1440-1681 (Electronic)
IS  - 0305-1870 (Linking)
VI  - 48
IP  - 11
DP  - 2021 Nov
TI  - Crosstalk between AdipoR1/AdipoR2 and Nrf2/HO-1 signal pathways activated by 
      beta-caryophyllene suppressed the compound 48/80 induced pseudo-allergic reactions.
PG  - 1523-1536
LID - 10.1111/1440-1681.13555 [doi]
AB  - Mast cell activation is initiated by two signalling pathways: immunoglobulin E 
      (IgE)-dependent and IgE-independent pathway. It is reported that the 
      IgE-independent type or pseudo-allergy pathway gets activated by 
      G-protein-dependent activation of the mast cell. Recently, adiponectin (APN) 
      receptors, AdipoR1, and AdipoR2 have been identified as G-protein-coupled 
      receptors (GPCRs). Interestingly, APN replenishment is reported to activate the 
      Nrf2/HO-1 signalling axis. However, no study has been performed interlinking the 
      role of APN and the Nrf2/HO-1 signalling axis in pseudo-allergic reaction. In the 
      present study, we evaluated the anti-pseudo-allergic effects of beta-caryophyllene, 
      an FDA-approved food additive, in activating AdipoR1/AdipoR2 and Nrf2/HO-1 axis 
      signalling pathway. Compound 48/80 (C48/80)-induced systemic and cutaneous 
      anaphylaxis-like shock in BALB/c mice was performed to assess the efficacy of 
      beta-caryophyllene (BCP). To assess the effect of BCP in anaphylactic hypotension, 
      mean arterial pressure was measured in Wistar rats. Inhibitory properties of BCP 
      in mast cell degranulation were estimated in rat peritoneal mast cells (RPMCs). 
      ELISA was performed to estimate interleukin (IL)-6, tumour necrosis factor (TNF), 
      IL-1beta, IgE, ovalbumin (OVA)-IgE and APN and western blotting for protein 
      expression of Nrf2/HO-1 and AdipoR1-AdipoR2. BCP dose-dependently inhibited 
      systemic and cutaneous anaphylaxis-like shock induced by C48/80. BCP 
      dose-dependently inhibited the mast cell degranulation followed by inhibition of 
      histamine release. Also BCP dose-dependently activated the Nrf2/HO-1 and 
      AdipoR1-AdipoR2 signalling axis pathway. Moreover, BCP reversed the drop in blood 
      pressure when the haemodynamic parameters were accessed. Our findings suggest 
      that BCP is a potent AdipoR1/AdipoR2-Nrf2/HO-1 axis pathway agonist that may 
      suppress the IgE-independent pathway towards allergic response to secretagogues.
CI  - (c) 2021 John Wiley & Sons Australia, Ltd.
FAU - Pathak, Manash Pratim
AU  - Pathak MP
AD  - Division of Pharmaceutical Technology, Defence Research Laboratory, Tezpur, 
      India.
AD  - Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh, India.
FAU - Patowary, Pompy
AU  - Patowary P
AD  - Division of Pharmaceutical Technology, Defence Research Laboratory, Tezpur, 
      India.
AD  - Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh, India.
FAU - Das, Aparoop
AU  - Das A
AD  - Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh, India.
FAU - Goyary, Danswrang
AU  - Goyary D
AD  - Division of Pharmaceutical Technology, Defence Research Laboratory, Tezpur, 
      India.
FAU - Karmakar, Sanjeev
AU  - Karmakar S
AD  - Division of Pharmaceutical Technology, Defence Research Laboratory, Tezpur, 
      India.
FAU - Bhutia, Yangchen D
AU  - Bhutia YD
AD  - Division of Pharmaceutical Technology, Defence Research Laboratory, Tezpur, 
      India.
FAU - Roy, Probin Kumar
AU  - Roy PK
AD  - Department of Pharmaceutics, Regional Institute of Paramedical and Nursing 
      Sciences, Aizawl, Mizoram, India.
FAU - Das, Sanghita
AU  - Das S
AD  - Division of Pharmaceutical Technology, Defence Research Laboratory, Tezpur, 
      India.
AD  - Pharmaceutical & Fine Chemical Division, Department of Chemical Technology, 
      University of Calcutta, Kolkata, India.
FAU - Chattopadhyay, Pronobesh
AU  - Chattopadhyay P
AUID- ORCID: 0000-0001-7770-6341
AD  - Division of Pharmaceutical Technology, Defence Research Laboratory, Tezpur, 
      India.
LA  - eng
GR  - University Grants Commission/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210815
PL  - Australia
TA  - Clin Exp Pharmacol Physiol
JT  - Clinical and experimental pharmacology & physiology
JID - 0425076
RN  - 4091-50-3 (p-Methoxy-N-methylphenethylamine)
SB  - IM
MH  - *p-Methoxy-N-methylphenethylamine
OTO - NOTNLM
OT  - Adipor1-AdipoR2
OT  - IgE-independent pathway
OT  - Nrf2/HO-1
OT  - allergic asthma
OT  - anaphylactic reactions
OT  - mast cell
OT  - ovalbumin
OT  - secretagogues
OT  - beta-caryophyllene
EDAT- 2021/07/28 06:00
MHDA- 2022/02/25 06:00
CRDT- 2021/07/27 17:23
PHST- 2021/07/09 00:00 [revised]
PHST- 2020/09/18 00:00 [received]
PHST- 2021/07/22 00:00 [accepted]
PHST- 2021/07/28 06:00 [pubmed]
PHST- 2022/02/25 06:00 [medline]
PHST- 2021/07/27 17:23 [entrez]
AID - 10.1111/1440-1681.13555 [doi]
PST - ppublish
SO  - Clin Exp Pharmacol Physiol. 2021 Nov;48(11):1523-1536. doi: 
      10.1111/1440-1681.13555. Epub 2021 Aug 15.