PMID- 34314863
OWN - NLM
STAT- MEDLINE
DCOM- 20210915
LR  - 20221221
IS  - 1873-1597 (Electronic)
IS  - 1572-1000 (Print)
IS  - 1572-1000 (Linking)
VI  - 35
DP  - 2021 Sep
TI  - Ultraviolet-A light reduces cellular cytokine release from human endotracheal 
      cells infected with Coronavirus.
PG  - 102457
LID - S1572-1000(21)00281-7 [pii]
LID - 10.1016/j.pdpdt.2021.102457 [doi]
AB  - BACKGROUND: An important clinical feature of coronavirus disease 2019 (COVID-19) 
      is hypercytokinemia (cytokine storm). We previously showed that narrow band 
      ultraviolet-A (NB-UVA) treatment salvages coronavirus (CoV)-229E-infected human 
      tracheal cells, and that daily endotracheal NB-UVA therapy reduced severe acute 
      respiratory syndrome coronavirus 2 (SARS-CoV-2) levels in human subjects, with 
      improved clinical outcomes. Here, we examined NB-UVA effects on cytokine release 
      during CoV-229E infection. METHODS: Primary human tracheal epithelial cells were 
      transfected with CoV-229E, then exposed to 2 mW/cm(2) NB-UVA for 20 minutes every 
      24h, either 3 or 4 times. Secreted cytokine/chemokine levels were analyzed in 
      supernatants collected from CoV-229E-infected/UVA-exposed cells 24h after the 
      last UVA treatment, and from matched non-infected/UVA-exposed controls, 
      CoV-229E-infected/non-exposed controls, and non-infected/non-exposed (naive) 
      controls. Metabolic pathway/downstream prediction analyses were also performed. 
      RESULTS: Pro-inflammatory cytokines interleukin (IL)-6 and tumor necrosis factor 
      (TNF), and chemokines IL-8, monocyte chemoattractant protein-1 (MCP1), and 
      interferon gamma-induced protein 10 (IP-10), were significantly increased in 
      CoV-229E-infected cells, and significantly decreased following NB-UVA treatment. 
      Interferon (IFN)-alpha2, IFN-gamma, and IL-10 were not upregulated in response to 
      CoV-229E. Metabolic pathway predictions indicated hypercytokinemia as the top 
      inflammatory response in CoV-229E-infected cells, whereas the top predicted 
      pathway in CoV-229E-infected/UVA-exposed cells was the recovery stage of severe 
      acute respiratory syndrome. CONCLUSIONS: Human tracheal epithelial cells infected 
      with CoV-229E showed reduced cytokine secretions including IL-6, TNF, IL-8, and 
      MCP-1, following NB-UVA exposure. This reduction of cytokine levels in vitro, 
      coupled with previously identified reduced cell death in 
      CoV-229E-infected/UVA-exposed cells, suggests that determining UVA effects on 
      cytokine storm in human SARS-Co-V2 patients is warranted.
CI  - Copyright (c) 2021. Published by Elsevier B.V.
FAU - Leite, Gabriela
AU  - Leite G
AD  - Medically Associated Science and Technology (MAST) Program, Cedars-Sinai, Los 
      Angeles, CA, USA.
FAU - Pimentel, Mark
AU  - Pimentel M
AD  - Medically Associated Science and Technology (MAST) Program, Cedars-Sinai, Los 
      Angeles, CA, USA; Karsh Division of Gastroenterology and Hepatology, 
      Cedars-Sinai, Los Angeles, CA, USA.
FAU - Mathur, Ruchi
AU  - Mathur R
AD  - Medically Associated Science and Technology (MAST) Program, Cedars-Sinai, Los 
      Angeles, CA, USA; Division of Endocrinology, Diabetes and Metabolism, Department 
      of Medicine, Cedars-Sinai, Los Angeles, CA, USA.
FAU - Barlow, Gillian M
AU  - Barlow GM
AD  - Medically Associated Science and Technology (MAST) Program, Cedars-Sinai, Los 
      Angeles, CA, USA.
FAU - Chan, Yin
AU  - Chan Y
AD  - Karsh Division of Gastroenterology and Hepatology, Cedars-Sinai, Los Angeles, CA, 
      USA.
FAU - Melmed, Gil Y
AU  - Melmed GY
AD  - Karsh Division of Gastroenterology and Hepatology, Cedars-Sinai, Los Angeles, CA, 
      USA.
FAU - Rezaie, Ali
AU  - Rezaie A
AD  - Medically Associated Science and Technology (MAST) Program, Cedars-Sinai, Los 
      Angeles, CA, USA; Karsh Division of Gastroenterology and Hepatology, 
      Cedars-Sinai, Los Angeles, CA, USA. Electronic address: ali.rezaie@cshs.org.
LA  - eng
PT  - Journal Article
DEP - 20210724
PL  - Netherlands
TA  - Photodiagnosis Photodyn Ther
JT  - Photodiagnosis and photodynamic therapy
JID - 101226123
RN  - 0 (Cytokines)
RN  - 0 (Photosensitizing Agents)
SB  - IM
MH  - *COVID-19
MH  - Cytokines
MH  - Humans
MH  - *Photochemotherapy/methods
MH  - Photosensitizing Agents
MH  - SARS-CoV-2
PMC - PMC8310417
OTO - NOTNLM
OT  - COVID-19
OT  - Coronavirus-229E
OT  - Cytokine storm
OT  - Human tracheal epithelial cells
OT  - Interleukin-6
OT  - Tumor necrosis factor
OT  - UV-A
OT  - Ultraviolet light-A
COIS- Cedars-Sinai has a patent on internal UV therapy, inventors: AR, MP, GM, RM and 
      GL. The rest of the authors declare no other financial conflicts of interest. 
      Cedars-Sinai has a licensing agreement with Aytu BioSciences.
EDAT- 2021/07/28 06:00
MHDA- 2021/09/16 06:00
PMCR- 2021/07/24
CRDT- 2021/07/27 20:17
PHST- 2021/06/16 00:00 [received]
PHST- 2021/07/12 00:00 [revised]
PHST- 2021/07/22 00:00 [accepted]
PHST- 2021/07/28 06:00 [pubmed]
PHST- 2021/09/16 06:00 [medline]
PHST- 2021/07/27 20:17 [entrez]
PHST- 2021/07/24 00:00 [pmc-release]
AID - S1572-1000(21)00281-7 [pii]
AID - 102457 [pii]
AID - 10.1016/j.pdpdt.2021.102457 [doi]
PST - ppublish
SO  - Photodiagnosis Photodyn Ther. 2021 Sep;35:102457. doi: 
      10.1016/j.pdpdt.2021.102457. Epub 2021 Jul 24.