PMID- 34319256
OWN - NLM
STAT- MEDLINE
DCOM- 20211217
LR  - 20221214
IS  - 1738-8872 (Electronic)
IS  - 1017-7825 (Print)
IS  - 1017-7825 (Linking)
VI  - 31
IP  - 9
DP  - 2021 Sep 28
TI  - Improvement of Biomineralization of Sporosarcina pasteurii as Biocementing 
      Material for Concrete Repair by Atmospheric and Room Temperature Plasma 
      Mutagenesis and Response Surface Methodology.
PG  - 1311-1322
LID - 10.4014/jmb.2104.04019 [doi]
AB  - Microbially induced calcium carbonate precipitation (MICP) has recently become an 
      intelligent and environmentally friendly method for repairing cracks in concrete. 
      To improve on this ability of microbial materials concrete repair, we applied 
      random mutagenesis and optimization of mineralization conditions to improve the 
      quantity and crystal form of microbially precipitated calcium carbonate. 
      Sporosarcina pasteurii ATCC 11859 was used as the starting strain to obtain the 
      mutant with high urease activity by atmospheric and room temperature plasma 
      (ARTP) mutagenesis. Next, we investigated the optimal biomineralization 
      conditions and precipitation crystal form using Plackett-Burman experimental 
      design and response surface methodology (RSM). Biomineralization with 0.73 mol/l 
      calcium chloride, 45 g/l urea, reaction temperature of 45 degrees C, and reaction time of 
      22 h, significantly increased the amount of precipitated calcium carbonate, which 
      was deposited in the form of calcite crystals. Finally, the repair of concrete 
      using the optimized biomineralization process was evaluated. A comparison of 
      water absorption and adhesion of concrete specimens before and after repairs 
      showed that concrete cracks and surface defects could be efficiently repaired. 
      This study provides a new method to engineer biocementing material for concrete 
      repair.
FAU - Han, Pei-Pei
AU  - Han PP
AD  - State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial 
      Fermentation Microbiology, Ministry of Education, College of Biotechnology, 
      Tianjin University of Science and Technology, Tianjin 300457, P.R. China.
FAU - Geng, Wen-Ji
AU  - Geng WJ
AD  - State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial 
      Fermentation Microbiology, Ministry of Education, College of Biotechnology, 
      Tianjin University of Science and Technology, Tianjin 300457, P.R. China.
FAU - Li, Meng-Nan
AU  - Li MN
AD  - State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial 
      Fermentation Microbiology, Ministry of Education, College of Biotechnology, 
      Tianjin University of Science and Technology, Tianjin 300457, P.R. China.
FAU - Jia, Shi-Ru
AU  - Jia SR
AD  - State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial 
      Fermentation Microbiology, Ministry of Education, College of Biotechnology, 
      Tianjin University of Science and Technology, Tianjin 300457, P.R. China.
FAU - Yin, Ji-Long
AU  - Yin JL
AD  - Tianjin Research Institute for Water Transportation Engineering, M.O.T., Tianjin 
      300456, P.R. China.
FAU - Xue, Run-Ze
AU  - Xue RZ
AD  - Tianjin Research Institute for Water Transportation Engineering, M.O.T., Tianjin 
      300456, P.R. China.
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - J Microbiol Biotechnol
JT  - Journal of microbiology and biotechnology
JID - 9431852
RN  - 0 (Plasma Gases)
RN  - 8W8T17847W (Urea)
RN  - EC 3.5.1.5 (Urease)
RN  - H0G9379FGK (Calcium Carbonate)
RN  - M4I0D6VV5M (Calcium Chloride)
RN  - Sporosarcina pasteurii
SB  - IM
MH  - Analysis of Variance
MH  - Biomineralization
MH  - Calcium Carbonate/chemistry/*metabolism
MH  - Calcium Chloride/chemistry/metabolism
MH  - Construction Materials/*microbiology
MH  - Mutagenesis
MH  - Mutation
MH  - Plasma Gases
MH  - Sporosarcina/genetics/*metabolism
MH  - Temperature
MH  - Urea/chemistry/metabolism
MH  - Urease/genetics/metabolism
PMC - PMC9705896
OTO - NOTNLM
OT  - Biomineralization
OT  - calcite
OT  - calcium carbonate
OT  - concrete repair
OT  - optimization
COIS- Conflict of Interest The authors have no financial conflicts of interest to 
      declare.
EDAT- 2021/07/29 06:00
MHDA- 2021/12/18 06:00
PMCR- 2021/08/03
CRDT- 2021/07/28 12:43
PHST- 2021/05/20 00:00 [received]
PHST- 2021/07/09 00:00 [revised]
PHST- 2021/07/28 00:00 [accepted]
PHST- 2021/07/29 06:00 [pubmed]
PHST- 2021/12/18 06:00 [medline]
PHST- 2021/07/28 12:43 [entrez]
PHST- 2021/08/03 00:00 [pmc-release]
AID - jmb.2104.04019 [pii]
AID - jmb-31-9-1311 [pii]
AID - 10.4014/jmb.2104.04019 [doi]
PST - ppublish
SO  - J Microbiol Biotechnol. 2021 Sep 28;31(9):1311-1322. doi: 10.4014/jmb.2104.04019.