PMID- 34319453
OWN - NLM
STAT- MEDLINE
DCOM- 20210913
LR  - 20210913
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 166
IP  - 10
DP  - 2021 Oct
TI  - The glycosyltransferase ST3GAL2 modulates virus proliferation and the 
      inflammation response in porcine reproductive and respiratory syndrome virus 
      infection.
PG  - 2723-2732
LID - 10.1007/s00705-021-05180-1 [doi]
AB  - beta-galactoside alpha-2,3-sialyltransferase 2 (ST3GAL2) is a member of the 
      sialyltransferase family that mediates terminal modification of glycoproteins and 
      glycolipids. ST3GAL2 has been found to play a role in obesity, aging, and 
      malignant diseases. In this study, we cloned porcine ST3GAL2 (pST3GAL2) from 
      porcine alveolar macrophages (PAMs), and its role in porcine reproductive and 
      respiratory syndrome virus (PRRSV) infection was investigated by transcriptome 
      analysis. pST3GAL2 was found to be located in the Golgi apparatus, and it was 
      expressed at high levels in PRRSV-infected PAMs. Overexpression of pST3GAL2 
      resulted in a slight increase in PRRSV proliferation, and the interaction between 
      pST3GAL2 and GP2a of PRRSV was detected by coimmunoprecipitation and confocal 
      microscopy. The expression of pro-inflammatory cytokines (IFN-beta, IL-2, IL-6, 
      IL-18, IL-1beta and TNF-alpha) was significantly inhibited in pST3GAL2-overexpressing, 
      PRRSV-infected cells and upregulated in PRRSV-infected pST3GAL2-knockout cells, 
      while the pattern of expression of anti-inflammatory cytokines (IL-4 and IL-10) 
      was diametrically opposite. Our results demonstrate that the regulation of 
      pST3GAL2 plays an important role in PRRSV proliferation and functional 
      alterations in virus-infected cells. These results contribute to our 
      understanding of the role of beta-galactoside alpha-2,3-sialyltransferase 2 in antiviral 
      immunity.
CI  - (c) 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, 
      part of Springer Nature.
FAU - Li, Xiaoyang
AU  - Li X
AD  - School of Life Sciences, Tianjin University, No. 92, Weijin Road, Nankai 
      District, Tianjin, 300072, China.
FAU - Guo, Yanyu
AU  - Guo Y
AD  - School of Life Sciences, Tianjin University, No. 92, Weijin Road, Nankai 
      District, Tianjin, 300072, China.
FAU - Song, Yinna
AU  - Song Y
AD  - School of Life Sciences, Tianjin University, No. 92, Weijin Road, Nankai 
      District, Tianjin, 300072, China.
FAU - Sun, Ruiqi
AU  - Sun R
AD  - School of Life Sciences, Tianjin University, No. 92, Weijin Road, Nankai 
      District, Tianjin, 300072, China.
FAU - Zhu, Min
AU  - Zhu M
AD  - School of Life Sciences, Tianjin University, No. 92, Weijin Road, Nankai 
      District, Tianjin, 300072, China.
FAU - Tan, Zheng
AU  - Tan Z
AD  - School of Life Sciences, Tianjin University, No. 92, Weijin Road, Nankai 
      District, Tianjin, 300072, China.
FAU - Swaiba, Umm E
AU  - Swaiba UE
AD  - School of Life Sciences, Tianjin University, No. 92, Weijin Road, Nankai 
      District, Tianjin, 300072, China.
FAU - Zhang, Lilin
AU  - Zhang L
AD  - School of Life Sciences, Tianjin University, No. 92, Weijin Road, Nankai 
      District, Tianjin, 300072, China.
FAU - Huang, Jinhai
AU  - Huang J
AUID- ORCID: 0000-0002-1073-533X
AD  - School of Life Sciences, Tianjin University, No. 92, Weijin Road, Nankai 
      District, Tianjin, 300072, China. jinhaih@tju.edu.cn.
LA  - eng
GR  - 20YFZCSN00340/the Key Projects of Science and Technology Support Grant of Tianjin 
      in China/
GR  - 2018YFD0500500/National Basic Research Program of China (973 Program) (CN)/
PT  - Journal Article
DEP - 20210728
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Cytokines)
RN  - 0 (Viral Envelope Proteins)
RN  - EC 2.4.99.- (Sialyltransferases)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cytokines/metabolism
MH  - Golgi Apparatus/metabolism
MH  - Inflammation
MH  - Macrophages, Alveolar/metabolism/virology
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/metabolism/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Sialyltransferases/genetics/*metabolism
MH  - Swine
MH  - Up-Regulation
MH  - Viral Envelope Proteins/metabolism
MH  - *Virus Replication
EDAT- 2021/07/29 06:00
MHDA- 2021/09/14 06:00
CRDT- 2021/07/28 12:51
PHST- 2021/01/26 00:00 [received]
PHST- 2021/05/10 00:00 [accepted]
PHST- 2021/07/29 06:00 [pubmed]
PHST- 2021/09/14 06:00 [medline]
PHST- 2021/07/28 12:51 [entrez]
AID - 10.1007/s00705-021-05180-1 [pii]
AID - 10.1007/s00705-021-05180-1 [doi]
PST - ppublish
SO  - Arch Virol. 2021 Oct;166(10):2723-2732. doi: 10.1007/s00705-021-05180-1. Epub 
      2021 Jul 28.