PMID- 34324170
OWN - NLM
STAT- MEDLINE
DCOM- 20211215
LR  - 20231108
IS  - 1678-4405 (Electronic)
IS  - 1517-8382 (Print)
IS  - 1517-8382 (Linking)
VI  - 52
IP  - 4
DP  - 2021 Dec
TI  - In vitro and in silico analysis reveals antifungal activity and potential targets 
      of curcumin on Paracoccidioides spp.
PG  - 1897-1911
LID - 10.1007/s42770-021-00548-6 [doi]
AB  - The search for new compounds with activity against Paracoccidioides, etiologic 
      agents of Paracoccidioidomycosis (PCM), is extremely necessary due to the current 
      scenario of the available therapeutic arsenal. Treatment is restricted to three 
      classes of antifungals with side effects. Curcumin is a polyphenol with 
      antifungal effects that is extracted from Curcuma longa. The present work aimed 
      to evaluate the activity of curcumin in different species of Paracoccidioides and 
      to evaluate the potential molecular targets of curcumin using computational 
      strategies. In addition, interactions with classic antifungals used in the 
      treatment of PCM were evaluated. Curcumin inhibits the growth of Paracoccidioides 
      spp. exerting a fungicidal effect. The combination of curcumin with amphotericin 
      B, co-trimoxazole, and itraconazole showed a synergistic or additive interaction. 
      Molecular targets as superoxide dismutase, catalase, and isocitrate lyase were 
      proposed based on in silico approaches. Curcumin affects the fungal plasma 
      membrane and increases the production of reactive oxygen species. Therefore, 
      curcumin is a good alternative for the treatment of PCM.
CI  - (c) 2021. Sociedade Brasileira de Microbiologia.
FAU - Rocha, Olivia Basso
AU  - Rocha OB
AD  - Laboratory of Molecular Biology, Institute of Biological Sciences, Federal 
      University of Goias, Avenida Esperanca, s/n, ICB2, Sala 206, Goiania, Goias, 
      74690-900, Brazil.
FAU - do Carmo Silva, Livia
AU  - do Carmo Silva L
AD  - Laboratory of Molecular Biology, Institute of Biological Sciences, Federal 
      University of Goias, Avenida Esperanca, s/n, ICB2, Sala 206, Goiania, Goias, 
      74690-900, Brazil.
FAU - de Carvalho Junior, Marcos Antonio Batista
AU  - de Carvalho Junior MAB
AD  - Laboratory of Molecular Biology, Institute of Biological Sciences, Federal 
      University of Goias, Avenida Esperanca, s/n, ICB2, Sala 206, Goiania, Goias, 
      74690-900, Brazil.
FAU - de Oliveira, Amanda Alves
AU  - de Oliveira AA
AD  - Laboratory of Molecular Biology, Institute of Biological Sciences, Federal 
      University of Goias, Avenida Esperanca, s/n, ICB2, Sala 206, Goiania, Goias, 
      74690-900, Brazil.
AD  - Institute of Tropical Pathology and Public Health, Federal University of Goias, 
      Goiania, Goias, Brazil.
FAU - de Almeida Soares, Celia Maria
AU  - de Almeida Soares CM
AD  - Laboratory of Molecular Biology, Institute of Biological Sciences, Federal 
      University of Goias, Avenida Esperanca, s/n, ICB2, Sala 206, Goiania, Goias, 
      74690-900, Brazil.
FAU - Pereira, Maristela
AU  - Pereira M
AUID- ORCID: 0000-0002-5482-8036
AD  - Laboratory of Molecular Biology, Institute of Biological Sciences, Federal 
      University of Goias, Avenida Esperanca, s/n, ICB2, Sala 206, Goiania, Goias, 
      74690-900, Brazil. maristelaufg@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20210729
PL  - Brazil
TA  - Braz J Microbiol
JT  - Brazilian journal of microbiology : [publication of the Brazilian Society for 
      Microbiology]
JID - 101095924
RN  - 0 (Antifungal Agents)
RN  - IT942ZTH98 (Curcumin)
SB  - IM
MH  - Antifungal Agents/pharmacology
MH  - Computer Simulation
MH  - *Curcumin/pharmacology/therapeutic use
MH  - Drug Synergism
MH  - Humans
MH  - In Vitro Techniques
MH  - *Paracoccidioides/drug effects
MH  - *Paracoccidioidomycosis/drug therapy/microbiology
PMC - PMC8578512
OTO - NOTNLM
OT  - Antifungal activity
OT  - Curcumin
OT  - Paracoccidioides spp.
COIS- The authors declare no competing interests.
EDAT- 2021/07/30 06:00
MHDA- 2021/12/16 06:00
PMCR- 2022/07/29
CRDT- 2021/07/29 12:33
PHST- 2020/12/08 00:00 [received]
PHST- 2021/06/21 00:00 [accepted]
PHST- 2021/07/30 06:00 [pubmed]
PHST- 2021/12/16 06:00 [medline]
PHST- 2021/07/29 12:33 [entrez]
PHST- 2022/07/29 00:00 [pmc-release]
AID - 10.1007/s42770-021-00548-6 [pii]
AID - 548 [pii]
AID - 10.1007/s42770-021-00548-6 [doi]
PST - ppublish
SO  - Braz J Microbiol. 2021 Dec;52(4):1897-1911. doi: 10.1007/s42770-021-00548-6. Epub 
      2021 Jul 29.