PMID- 34326390
OWN - NLM
STAT- MEDLINE
DCOM- 20211213
LR  - 20240403
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 11
IP  - 1
DP  - 2021 Jul 29
TI  - Ontology of the apelinergic system in mouse pancreas during pregnancy and 
      relationship with beta-cell mass.
PG  - 15475
LID - 10.1038/s41598-021-94725-0 [doi]
LID - 15475
AB  - The apelin receptor (Aplnr) and its ligands, Apelin and Apela, contribute to 
      metabolic control. The insulin resistance associated with pregnancy is 
      accommodated by an expansion of pancreatic beta-cell mass (BCM) and increased 
      insulin secretion, involving the proliferation of insulin-expressing, glucose 
      transporter 2-low (Ins(+)Glut2(LO)) progenitor cells. We examined changes in the 
      apelinergic system during normal mouse pregnancy and in pregnancies complicated 
      by glucose intolerance with reduced BCM. Expression of Aplnr, Apelin and Apela 
      was quantified in Ins(+)Glut2(LO) cells isolated from mouse pancreata and found 
      to be significantly higher than in mature beta-cells by DNA microarray and qPCR. 
      Apelin was localized to most beta-cells by immunohistochemistry although Aplnr was 
      predominantly associated with Ins(+)Glut2(LO) cells. Aplnr-staining cells 
      increased three- to four-fold during pregnancy being maximal at gestational days 
      (GD) 9-12 but were significantly reduced in glucose intolerant mice. Apelin-13 
      increased beta-cell proliferation in isolated mouse islets and INS1E cells, but not 
      glucose-stimulated insulin secretion. Glucose intolerant pregnant mice had 
      significantly elevated serum Apelin levels at GD 9 associated with an increased 
      presence of placental IL-6. Placental expression of the apelinergic axis remained 
      unaltered, however. Results show that the apelinergic system is highly expressed 
      in pancreatic beta-cell progenitors and may contribute to beta-cell proliferation in 
      pregnancy.
CI  - (c) 2021. The Author(s).
FAU - Strutt, Brenda
AU  - Strutt B
AD  - Lawson Health Research Institute, St Joseph Health Care, 268 Grosvenor St, 
      London, ON, N6A 4V2, Canada.
FAU - Szlapinski, Sandra
AU  - Szlapinski S
AD  - Lawson Health Research Institute, St Joseph Health Care, 268 Grosvenor St, 
      London, ON, N6A 4V2, Canada.
AD  - Department of Physiology and Pharmacology, Western University, London, ON, N6A 
      3K7, Canada.
FAU - Gnaneswaran, Thineesha
AU  - Gnaneswaran T
AD  - Department of Physiology and Pharmacology, Western University, London, ON, N6A 
      3K7, Canada.
FAU - Donegan, Sarah
AU  - Donegan S
AD  - Department of Physiology and Pharmacology, Western University, London, ON, N6A 
      3K7, Canada.
FAU - Hill, Jessica
AU  - Hill J
AD  - Institute of Biomedical and Clinical Science, University of Exeter Medical 
      School, Exeter, EX2 5DW, UK.
FAU - Bennett, Jamie
AU  - Bennett J
AD  - Lawson Health Research Institute, St Joseph Health Care, 268 Grosvenor St, 
      London, ON, N6A 4V2, Canada.
AD  - Life Sciences Program, School of Interdisciplinary Science, McMaster University, 
      Hamilton, ON, L8S 4LD, Canada.
FAU - Hill, David J
AU  - Hill DJ
AUID- ORCID: 0000-0002-2490-5678
AD  - Lawson Health Research Institute, St Joseph Health Care, 268 Grosvenor St, 
      London, ON, N6A 4V2, Canada. david.hill@lawsonresearch.com.
AD  - Department of Physiology and Pharmacology, Western University, London, ON, N6A 
      3K7, Canada. david.hill@lawsonresearch.com.
AD  - Departments of Medicine and Paediatrics, Western University, London, ON, N6A 3K7, 
      Canada. david.hill@lawsonresearch.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210729
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Apelin)
RN  - 0 (Apelin Receptors)
RN  - 0 (Insulin)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Interleukin-6)
RN  - 0 (apelin-13 peptide)
RN  - 0 (interleukin-6, mouse)
SB  - IM
MH  - Animals
MH  - Apelin/metabolism
MH  - Apelin Receptors/metabolism
MH  - Cell Proliferation
MH  - Cell Separation
MH  - Female
MH  - Flow Cytometry
MH  - Gene Expression Profiling
MH  - Glucose Intolerance
MH  - Insulin/*metabolism
MH  - Insulin Resistance
MH  - Insulin-Secreting Cells/*metabolism
MH  - Intercellular Signaling Peptides and Proteins/metabolism
MH  - Interleukin-6/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Oligonucleotide Array Sequence Analysis
MH  - Pancreas/*embryology
MH  - Placenta/metabolism
MH  - Pregnancy
MH  - *Pregnancy, Animal
PMC - PMC8322410
COIS- The study funders were not involved in the design of the study; the collection, 
      analysis, and interpretation of data; writing the report; and did not impose any 
      restrictions regarding the publication of the report. The authors had no 
      financial or professional conflicts of interest with the funding agencies.
EDAT- 2021/07/31 06:00
MHDA- 2021/12/15 06:00
PMCR- 2021/07/29
CRDT- 2021/07/30 06:14
PHST- 2021/04/11 00:00 [received]
PHST- 2021/07/15 00:00 [accepted]
PHST- 2021/07/30 06:14 [entrez]
PHST- 2021/07/31 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2021/07/29 00:00 [pmc-release]
AID - 10.1038/s41598-021-94725-0 [pii]
AID - 94725 [pii]
AID - 10.1038/s41598-021-94725-0 [doi]
PST - epublish
SO  - Sci Rep. 2021 Jul 29;11(1):15475. doi: 10.1038/s41598-021-94725-0.