PMID- 34333004
OWN - NLM
STAT- MEDLINE
DCOM- 20220103
LR  - 20240226
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 187
DP  - 2021 Sep 30
TI  - Development of a monoclonal antibody against PRRSV glycoprotein 3 using an 
      immuodominant peptide as immunogen.
PG  - 683-689
LID - S0141-8130(21)01619-6 [pii]
LID - 10.1016/j.ijbiomac.2021.07.168 [doi]
AB  - Glycoprotein 3 (GP3), a highly glycosylated membrane protein, is a protective 
      antigen and minor structural protein of porcine reproductive and respiratory 
      syndrome virus (PRRSV), and plays a crucial role in virus assembly and infection. 
      In the present study, we synthesized 23 overlapping peptides span GP3 protein 
      sequence and used pig anti-PRRSV serums to identify immunodominant peptides by 
      indirect ELISA. Five immunodominant peptides GP3-P3, P4, P5, P6 and P7 were 
      identified and GP3-P4 (P(55)LCPTRQAAAEILEPGKS(72)) was conjugated to carrier 
      protein BSA. One mAb 1E5 against GP3 was generated from BALB/c mice immunized 
      with the conjugates BSA-P4. The Characterization of mAb was identified by Western 
      blot, Dot-ELISA, IPMA and IFA. We found that mAb 1E5 can specifically react with 
      HP-PRRSV strains but not C-PRRSV or NADC30-like PRRSV strains tested in this 
      study. Site-directed alanine substitution analysis revealed that 8 amino acid 
      residues were involved in antibody binding, among them E65, L67 and P69 were 
      critical residue recognized by mAb 1E5. Taken together, this study provided a 
      novel strategy for generating specific mAbs against virus proteins by using 
      immunodominant peptides as targets, and the mAb 1E5 may be useful for development 
      of rapid differential detection method differentiating HP-PRRSV from C-PRRSV and 
      NADC30-like PRRSV.
CI  - Copyright (c) 2021. Published by Elsevier B.V.
FAU - Liang, Chao
AU  - Liang C
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou 450001, Henan, China.
FAU - Liu, Hongliang
AU  - Liu H
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou 450001, Henan, China.
FAU - Zhou, Jingming
AU  - Zhou J
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou 450001, Henan, China.
FAU - Chen, Yumei
AU  - Chen Y
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou 450001, Henan, China.
FAU - Ding, Peiyang
AU  - Ding P
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou 450001, Henan, China.
FAU - Zhu, Xifang
AU  - Zhu X
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou 450001, Henan, China.
FAU - Wang, Mengmeng
AU  - Wang M
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou 450001, Henan, China.
FAU - Ding, Menghao
AU  - Ding M
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou 450001, Henan, China.
FAU - Wang, Aiping
AU  - Wang A
AD  - School of Life Sciences, Zhengzhou University, Zhengzhou 450001, Henan, China. 
      Electronic address: pingaw@126.com.
LA  - eng
PT  - Journal Article
DEP - 20210730
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Viral)
RN  - 0 (Glycoproteins)
RN  - 0 (Immunodominant Epitopes)
RN  - 0 (Peptide Fragments)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*immunology
MH  - Antibody Specificity
MH  - Antigens, Viral/administration & dosage/genetics/*immunology
MH  - Cell Line, Tumor
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epitope Mapping
MH  - Female
MH  - Glycoproteins/administration & dosage/genetics/*immunology
MH  - Immunization
MH  - *Immunodominant Epitopes
MH  - Mice, Inbred BALB C
MH  - Peptide Fragments/administration & dosage/genetics/*immunology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*immunology
MH  - Sus scrofa
MH  - Viral Proteins/administration & dosage/genetics/*immunology
MH  - Mice
OTO - NOTNLM
OT  - GP3
OT  - Glycosylated membrane protein
OT  - Immunodominant peptides
OT  - Monoclonal antibodies
OT  - PRRSV
EDAT- 2021/08/02 06:00
MHDA- 2022/01/04 06:00
CRDT- 2021/08/01 20:41
PHST- 2021/04/13 00:00 [received]
PHST- 2021/07/25 00:00 [revised]
PHST- 2021/07/26 00:00 [accepted]
PHST- 2021/08/02 06:00 [pubmed]
PHST- 2022/01/04 06:00 [medline]
PHST- 2021/08/01 20:41 [entrez]
AID - S0141-8130(21)01619-6 [pii]
AID - 10.1016/j.ijbiomac.2021.07.168 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2021 Sep 30;187:683-689. doi: 
      10.1016/j.ijbiomac.2021.07.168. Epub 2021 Jul 30.