PMID- 34335696
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210803
IS  - 1664-8021 (Print)
IS  - 1664-8021 (Electronic)
IS  - 1664-8021 (Linking)
VI  - 12
DP  - 2021
TI  - Identification of Genomic Regions Influencing N-Metabolism and N-Excretion in 
      Lactating Holstein- Friesians.
PG  - 699550
LID - 10.3389/fgene.2021.699550 [doi]
LID - 699550
AB  - Excreted nitrogen (N) of dairy cows contribute to environmental eutrophication. 
      The main N-excretory metabolite of dairy cows is urea, which is synthesized as a 
      result of N-metabolization in the liver and is excreted via milk and urine. 
      Genetic variation in milk urea (MU) has been postulated but the complex 
      physiology behind the trait as well as the tremendous diversity of processes 
      regulating the N-metabolism impede the consistent determination of causal regions 
      in the bovine genome. In order to map the genetic determinants affecting 
      N-excretion, MU and eight other N-excretory metabolites in milk and urine were 
      assessed in a genome-wide association study. Therefore phenotypes of 371 
      Holstein- Friesians were obtained in a trial on a dairy farm under near 
      commercial conditions. Genotype data comprised SNP information of the Bovine 50K 
      MD Genome chip (45,613 SNPs). Significantly associated genomic regions for MU 
      concentration revealed GJA1 (BTA 9), RXFP1, and FRY1 (both BTA 12) as putative 
      candidates. For milk urea yield (MUY) a promising QTL on BTA 17 including SH3D19 
      emerged, whereas RCAN2, CLIC5, ENPP4, and ENPP5 (BTA 23) are suggested to 
      influence urinary urea concentration. Minor N-fractions in milk (MN) may be 
      regulated by ELF2 and SLC7A11 (BTA 17), whilst ITPR2 and MYBPC1 (BTA 5), STIM2 
      (BTA 6), SGCD (BTA 7), SLC6A2 (BTA 18), TMCC2 and MFSD4A (BTA 16) are suggested 
      to have an impact on various non-urea-N (NUN) fractions excreted via urine. Our 
      results highlight genomic regions and candidate genes for N-excretory metabolites 
      and provide a deeper insight into the predisposed component to regulate the 
      N-metabolism in dairy cows.
CI  - Copyright (c) 2021 Honerlagen, Reyer, Oster, Ponsuksili, Trakooljul, Kuhla, Reinsch 
      and Wimmers.
FAU - Honerlagen, Hanne
AU  - Honerlagen H
AD  - Genomics Unit, Institute for Genome Biology, Leibniz Institute for Farm Animal 
      Biology (FBN), Dummerstorf, Germany.
FAU - Reyer, Henry
AU  - Reyer H
AD  - Genomics Unit, Institute for Genome Biology, Leibniz Institute for Farm Animal 
      Biology (FBN), Dummerstorf, Germany.
FAU - Oster, Michael
AU  - Oster M
AD  - Genomics Unit, Institute for Genome Biology, Leibniz Institute for Farm Animal 
      Biology (FBN), Dummerstorf, Germany.
FAU - Ponsuksili, Siriluck
AU  - Ponsuksili S
AD  - Genomics Unit, Institute for Genome Biology, Leibniz Institute for Farm Animal 
      Biology (FBN), Dummerstorf, Germany.
FAU - Trakooljul, Nares
AU  - Trakooljul N
AD  - Genomics Unit, Institute for Genome Biology, Leibniz Institute for Farm Animal 
      Biology (FBN), Dummerstorf, Germany.
FAU - Kuhla, Bjorn
AU  - Kuhla B
AD  - Metabolism Efficiency Unit, Institute of Nutritional Physiology "Oskar Kellner," 
      Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, Germany.
FAU - Reinsch, Norbert
AU  - Reinsch N
AD  - Livestock Genetics and Breeding Unit, Institute of Genetics and Biometry, Leibniz 
      Institute for Farm Animal Biology (FBN), Dummerstorf, Germany.
FAU - Wimmers, Klaus
AU  - Wimmers K
AD  - Genomics Unit, Institute for Genome Biology, Leibniz Institute for Farm Animal 
      Biology (FBN), Dummerstorf, Germany.
AD  - Faculty of Agricultural and Environmental Sciences, University of Rostock, 
      Rostock, Germany.
LA  - eng
PT  - Journal Article
DEP - 20210714
PL  - Switzerland
TA  - Front Genet
JT  - Frontiers in genetics
JID - 101560621
PMC - PMC8318802
OTO - NOTNLM
OT  - GWAS
OT  - cattle
OT  - emission
OT  - milk urea
OT  - nitrogen
OT  - non-urea nitrogen
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/08/03 06:00
MHDA- 2021/08/03 06:01
PMCR- 2021/07/14
CRDT- 2021/08/02 05:56
PHST- 2021/04/23 00:00 [received]
PHST- 2021/06/18 00:00 [accepted]
PHST- 2021/08/02 05:56 [entrez]
PHST- 2021/08/03 06:00 [pubmed]
PHST- 2021/08/03 06:01 [medline]
PHST- 2021/07/14 00:00 [pmc-release]
AID - 10.3389/fgene.2021.699550 [doi]
PST - epublish
SO  - Front Genet. 2021 Jul 14;12:699550. doi: 10.3389/fgene.2021.699550. eCollection 
      2021.