PMID- 34336357
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210803
IS  - 2090-8865 (Print)
IS  - 2090-8873 (Electronic)
IS  - 2090-8873 (Linking)
VI  - 2021
DP  - 2021
TI  - Impact of Different Extraction Methods on Furanosesquiterpenoids Content and 
      Antibacterial Activity of Commiphora myrrha Resin.
PG  - 5525173
LID - 10.1155/2021/5525173 [doi]
LID - 5525173
AB  - The oleo-gum-resin of Commiphora myrrha is one of the most known natural 
      antimicrobial agents, mainly due to its furanosesquiterpenes. A validated method 
      based on sample extraction by matrix solid-phase dispersion (MSPD) followed by 
      high-performance column chromatography (HPLC) determination is applied to analyze 
      two furanosesquiterpenoids, namely, 2-methoxyfuranodiene (CM-1) and 
      2-acetoxyfuranodiene (CM-2), existing in C. myrrha. The trial parameters that 
      controlled the extraction prospective were studied and optimized. These include 
      the nature of dispersant, mass ratio of sample to the dispersant, and the volume 
      of elution solvent. A comparative antimicrobial study that used the Minimum 
      Inhibitory Concentration Assay (MIC) method between MSPD, ultrasonic, and Soxhlet 
      of myrrh extracts was also conducted. The optimal MSPD parameters used were (i) 
      15 mL of methanol applied as elution solvent; (ii) silica gel/sample mass at a 
      2 : 1 ratio; and (iii) a dispersing sorbent selected as silica gel. Technique 
      retrievals were regulated from 96.87% to 100.54%, with relative standard 
      deviations (RSDs) from 1.24% to 4.45%. Commiphora myrrha-MSPD (CM-MSPD) extract 
      showed the highest antibacterial activity against gram-positive and gram-negative 
      bacteria (156.25 mug/mL and 312.5 mug/mL, respectively) and antifungal activity 
      (156.25 mug/mL). Yields acquired through the MSPD technique were larger than 
      yields from other extraction techniques (sonication and traditional reflux 
      extraction methods) with less consumption of time, sample, and solvent. The mode 
      of antibacterial action of CM-1 and CM-2 was elucidated by performing molecular 
      docking with bacterial DNA gyrase. Both the compounds interacted with key 
      residues of DNA gyrase.
CI  - Copyright (c) 2021 Ali S. Alqahtani et al.
FAU - Alqahtani, Ali S
AU  - Alqahtani AS
AUID- ORCID: 0000-0002-1034-7441
AD  - Department of Pharmacognosy, College of Pharmacy, King Saud University, P. O. Box 
      2457, Riyadh 11451, Saudi Arabia.
FAU - Herqash, Rashed N
AU  - Herqash RN
AUID- ORCID: 0000-0002-4502-9507
AD  - Department of Pharmacognosy, College of Pharmacy, King Saud University, P. O. Box 
      2457, Riyadh 11451, Saudi Arabia.
FAU - Noman, Omar M
AU  - Noman OM
AUID- ORCID: 0000-0003-0902-6381
AD  - Department of Pharmacognosy, College of Pharmacy, King Saud University, P. O. Box 
      2457, Riyadh 11451, Saudi Arabia.
FAU - Tabish Rehman, Md
AU  - Tabish Rehman M
AD  - Department of Pharmacognosy, College of Pharmacy, King Saud University, P. O. Box 
      2457, Riyadh 11451, Saudi Arabia.
FAU - Shahat, Abdelaaty A
AU  - Shahat AA
AD  - Department of Pharmacognosy, College of Pharmacy, King Saud University, P. O. Box 
      2457, Riyadh 11451, Saudi Arabia.
FAU - Alajmi, Mohamed F
AU  - Alajmi MF
AD  - Department of Pharmacognosy, College of Pharmacy, King Saud University, P. O. Box 
      2457, Riyadh 11451, Saudi Arabia.
FAU - Nasr, Fahd A
AU  - Nasr FA
AUID- ORCID: 0000-0002-6496-7822
AD  - Department of Pharmacognosy, College of Pharmacy, King Saud University, P. O. Box 
      2457, Riyadh 11451, Saudi Arabia.
LA  - eng
PT  - Journal Article
DEP - 20210710
PL  - Egypt
TA  - J Anal Methods Chem
JT  - Journal of analytical methods in chemistry
JID - 101575377
PMC - PMC8289610
COIS- The authors declare that they do not have any conflicts of interest regarding the 
      publication of this paper.
EDAT- 2021/08/03 06:00
MHDA- 2021/08/03 06:01
PMCR- 2021/07/10
CRDT- 2021/08/02 06:04
PHST- 2021/02/23 00:00 [received]
PHST- 2021/03/25 00:00 [revised]
PHST- 2021/06/24 00:00 [accepted]
PHST- 2021/08/02 06:04 [entrez]
PHST- 2021/08/03 06:00 [pubmed]
PHST- 2021/08/03 06:01 [medline]
PHST- 2021/07/10 00:00 [pmc-release]
AID - 10.1155/2021/5525173 [doi]
PST - epublish
SO  - J Anal Methods Chem. 2021 Jul 10;2021:5525173. doi: 10.1155/2021/5525173. 
      eCollection 2021.