PMID- 34337939
OWN - NLM
STAT- MEDLINE
DCOM- 20210812
LR  - 20220531
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 69
IP  - 31
DP  - 2021 Aug 11
TI  - Fab Fragment of Immunoglobulin Y Modulates NF-kappaB and MAPK Signaling through TLR4 
      and alphaVbeta3 Integrin and Inhibits the Inflammatory Effect on R264.7 Macrophages.
PG  - 8747-8757
LID - 10.1021/acs.jafc.1c03330 [doi]
AB  - High-purity Fab fragment and immunoglobulin Y (IgY) were prepared to evaluate 
      their anti-inflammatory activity in the lipopolysaccharide (LPS)-induced Raw 
      264.7 macrophage system. Compared with IgY, the Fab fragment possessed a greater 
      potency in inhibiting the inflammation by nitric oxide (NO)/inducible nitric 
      oxide synthase (iNOS) and prostaglandin-E2 (PGE2)/cyclooxygenase-2 (COX-2) 
      pathways. The Fab fragment attenuated the levels of tumor necrosis factor-alpha 
      (TNF-alpha), interleukin-6 (IL-6), and interleukin-10 (IL-10) to 38.07 +/- 1.86-48.39 +/- 
      11.33 pg/mL (63.1-71.0% inhibition), 31.59 +/- 3.91-38.08 +/- 4.44 pg/mL (72.4-77.1% 
      inhibition), and 20.62 +/- 0.46-21.91 +/- 0.65 pg/mL (50-53% inhibition), 
      respectively. Additionally, the Fab fragment significantly inhibited the 
      translocation of nuclear transcription factor-kappaB (NF-kappaB) p65 and the 
      phosphorylation of mitogen-activated protein kinase (MAPK) proteins, including 
      ERK1/2 (41.5/33.2%), JNK1/2 (44.2/39.6%), and p38 (42.2%). The Fab fragment could 
      be internalized into cells, and the pretreatment of RAW 264.7 macrophages with 
      the Fab fragment reduced the mRNA expression of the Toll-like receptor (TLR4, 
      32.7-44.4% inhibition) and alphaVbeta3 integrin (76.1% inhibition). In conclusion, Fab 
      fragments regulated the TLR4 and alphaVbeta3 integrin-mediated inflammatory processes by 
      blocking the NF-kappaB and MAPKs pathways in the LPS-induced RAW 264.7 macrophage 
      system.
FAU - Zhou, Xin
AU  - Zhou X
AD  - Key Laboratory of Environment Correlative Dietology, National Research and 
      Development Centre for Egg Processing, College of Food Science and Technology, 
      Huazhong Agricultural University, Wuhan 430070, China.
FAU - Ahn, Dong Uk
AU  - Ahn DU
AD  - Department of Animal Science, Iowa State University, Ames, Iowa 50011, United 
      States.
FAU - Xia, Minquan
AU  - Xia M
AD  - Key Laboratory of Environment Correlative Dietology, National Research and 
      Development Centre for Egg Processing, College of Food Science and Technology, 
      Huazhong Agricultural University, Wuhan 430070, China.
FAU - Zeng, Qi
AU  - Zeng Q
AD  - Key Laboratory of Environment Correlative Dietology, National Research and 
      Development Centre for Egg Processing, College of Food Science and Technology, 
      Huazhong Agricultural University, Wuhan 430070, China.
FAU - Li, Xiaomeng
AU  - Li X
AD  - Key Laboratory of Environment Correlative Dietology, National Research and 
      Development Centre for Egg Processing, College of Food Science and Technology, 
      Huazhong Agricultural University, Wuhan 430070, China.
FAU - Cai, Zhaoxia
AU  - Cai Z
AUID- ORCID: 0000-0002-8441-6446
AD  - Key Laboratory of Environment Correlative Dietology, National Research and 
      Development Centre for Egg Processing, College of Food Science and Technology, 
      Huazhong Agricultural University, Wuhan 430070, China.
LA  - eng
PT  - Journal Article
DEP - 20210802
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (IgY)
RN  - 0 (Immunoglobulin Fab Fragments)
RN  - 0 (Immunoglobulins)
RN  - 0 (Integrin alpha5)
RN  - 0 (Integrins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (Tlr4 protein, mouse)
RN  - 0 (Toll-Like Receptor 4)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Cyclooxygenase 2/metabolism
MH  - Immunoglobulin Fab Fragments/*immunology
MH  - Immunoglobulins
MH  - Integrin alpha5
MH  - Integrins
MH  - Lipopolysaccharides
MH  - Macrophages/metabolism
MH  - Mice
MH  - Mitogen-Activated Protein Kinases/genetics
MH  - *NF-kappa B/genetics/metabolism
MH  - Nitric Oxide/metabolism
MH  - Nitric Oxide Synthase Type II/metabolism
MH  - RAW 264.7 Cells
MH  - *Toll-Like Receptor 4/genetics/metabolism
OTO - NOTNLM
OT  - Fab fragment
OT  - IgY
OT  - TLR4
OT  - anti-inflammation
OT  - alphaVbeta3 integrin
EDAT- 2021/08/03 06:00
MHDA- 2021/08/13 06:00
CRDT- 2021/08/02 08:41
PHST- 2021/08/03 06:00 [pubmed]
PHST- 2021/08/13 06:00 [medline]
PHST- 2021/08/02 08:41 [entrez]
AID - 10.1021/acs.jafc.1c03330 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2021 Aug 11;69(31):8747-8757. doi: 10.1021/acs.jafc.1c03330. 
      Epub 2021 Aug 2.