PMID- 34344057
OWN - NLM
STAT- MEDLINE
DCOM- 20220524
LR  - 20220602
IS  - 1758-2229 (Electronic)
IS  - 1758-2229 (Linking)
VI  - 14
IP  - 3
DP  - 2022 Jun
TI  - Genotypes and phenotypes of methicillin-resistant staphylococci isolated from 
      shrimp aquaculture farms.
PG  - 391-399
LID - 10.1111/1758-2229.12995 [doi]
AB  - The population of methicillin-resistant (MR) staphylococci in aquatic environment 
      is rarely investigated. Here, we characterized a collection of MR staphylococci 
      recovered from shrimp aquaculture farms (n = 37) in Kerala, India. A total of 261 
      samples yielded 47 MR isolates (16 S. aureus, 13 S. haemolyticus, 11 S. 
      epidermidis, 3 S. saprophytics and 2 each of S.intermedius and S. kloosii). 
      Multi-drug resistance was evident in 72.3% of the isolates, with resistance 
      mainly towards erythromycin (78.7%), norfloxacin and 
      trimethoprim-sulfamethoxazole (53.2%), and gentamicin (34%). Major resistance 
      genes identified included mecA (100%), ermC (38.3%), aacA-aphD (21.3%), tetK 
      (14.9%) and tetM (21.3%). Almost 60% of the isolates carried type V SCCmec 
      (Staphylococcal Cassette Chromosome mec), and the remaining harboured untypeable 
      SCCmec elements. Comprehensive genotyping of the methicillin-resistant 
      Staphylococcus aureus isolates revealed high prevalence of ST772-t345-V (sequence 
      type-spa type-SCCmec type) (75%), followed by minor representations of 
      ST6657-t345-V and ST3190-t12353. The isolates of S. haemolyticus and S. 
      epidermidis were genotypically diverse as shown by their pulsed-field gel 
      electrophoresis (PFGE) profiles. Genes encoding staphylococcal enterotoxins were 
      observed in 53.2% of the isolates. Various genes involved in adhesion and biofilm 
      formation were also identified. In conclusion, our findings provide evidence that 
      shrimp aquaculture settings can act as reservoirs of methicillin-resistant 
      staphylococci.
CI  - (c) 2021 Society for Applied Microbiology and John Wiley & Sons Ltd.
FAU - Rajan, Vineeth
AU  - Rajan V
AUID- ORCID: 0000-0003-0648-1610
AD  - Microbiology, Fermentation and Biotechnology Division, ICAR-Central Institute of 
      Fisheries Technology, Cochin, Kerala, India.
FAU - Sivaraman, Gopalan Krishnan
AU  - Sivaraman GK
AD  - Microbiology, Fermentation and Biotechnology Division, ICAR-Central Institute of 
      Fisheries Technology, Cochin, Kerala, India.
FAU - Vijayan, Ardhra
AU  - Vijayan A
AD  - Microbiology, Fermentation and Biotechnology Division, ICAR-Central Institute of 
      Fisheries Technology, Cochin, Kerala, India.
FAU - Elangovan, Ravikrishnan
AU  - Elangovan R
AD  - Department of Biochemical Engineering and Biotechnology, Indian Institute of 
      Technology Delhi, New Delhi, India.
FAU - Prendiville, Alison
AU  - Prendiville A
AD  - Division of Infection and Pathway Medicine, Edinburgh Medical School, University 
      of Edinburgh, Edinburgh, UK.
FAU - Bachmann, Till T
AU  - Bachmann TT
AD  - London College of Communication, University of the Arts London, London, UK.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210803
PL  - United States
TA  - Environ Microbiol Rep
JT  - Environmental microbiology reports
JID - 101499207
RN  - 0 (Anti-Bacterial Agents)
SB  - IM
MH  - Anti-Bacterial Agents/pharmacology
MH  - Aquaculture
MH  - Genotype
MH  - Methicillin Resistance/genetics
MH  - *Methicillin-Resistant Staphylococcus aureus/genetics
MH  - Microbial Sensitivity Tests
MH  - Phenotype
MH  - Staphylococcus/genetics
MH  - Staphylococcus aureus
EDAT- 2021/08/04 06:00
MHDA- 2022/05/25 06:00
CRDT- 2021/08/03 20:32
PHST- 2021/07/18 00:00 [revised]
PHST- 2021/05/19 00:00 [received]
PHST- 2021/07/19 00:00 [accepted]
PHST- 2021/08/04 06:00 [pubmed]
PHST- 2022/05/25 06:00 [medline]
PHST- 2021/08/03 20:32 [entrez]
AID - 10.1111/1758-2229.12995 [doi]
PST - ppublish
SO  - Environ Microbiol Rep. 2022 Jun;14(3):391-399. doi: 10.1111/1758-2229.12995. Epub 
      2021 Aug 3.