PMID- 34345289
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240228
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Print)
IS  - 1792-0981 (Linking)
VI  - 22
IP  - 3
DP  - 2021 Sep
TI  - Knockdown of circ_0001883 may inhibit epithelial-mesenchymal transition in 
      laryngeal squamous cell carcinoma via the miR-125-5p/PI3K/AKT axis.
PG  - 1007
LID - 10.3892/etm.2021.10440 [doi]
LID - 1007
AB  - Laryngeal squamous cell carcinoma (LSCC) is a malignant tumor with increasing 
      incidence and poor prognosis. Circular RNAs (circRNAs) are known to modulate 
      tumorigenesis and cancer development that may function through microRNAs (miRs). 
      The aim of the present study was to investigate the functional roles of 
      circ_0001883 in LSCC and the underlying molecular mechanism. The expression of 
      circ_0001883 was upregulated and measured using reverse 
      transcription-quantitative PCR (RT-qPCR) and RNase R. miR-125b-5p expression was 
      downregulated in LSCC tissues and cells as determined using RT-qPCR. 
      Subsequently, knockdown of circ_0001883 inhibited LSCC cell migration, invasion 
      and epithelial-mesenchymal transition (EMT), which were tested by wound healing 
      assays, Transwell assays and western blotting, respectively. Bioinformatics 
      analysis predicted that circ_0001883 was a sponge of miR-125b-5p, which was 
      verified using a dual-luciferase reporter assay. Knockdown of circ_0001883 played 
      a functional role by sponging miR-125b-5p. Additionally, circ_0001883 and 
      miR-125b-5p influenced phosphorylation of PI3K and AKT, detected via western 
      blotting. In an in vivo study, knockdown of circ_0001883 reduced tumor volume and 
      weight in mice, along with enhanced miR-125b-5p and E-cadherin expression levels, 
      and decreased N-cadherin, phosphorylated (p)-PI3K/PI3K and p-AKT/AKT ratios. In 
      conclusion, knockdown of circ_0001883 inhibited cell migration, invasion and EMT 
      of LSCC by sponging miR-125b-5p. This is hypothesized to be via the PI3K/AKT 
      signaling pathway, which suggested that circ_0001883 has potential for LSCC 
      therapy.
CI  - Copyright: (c) Chen et al.
FAU - Chen, Fu
AU  - Chen F
AD  - Department of Radiation Oncology, Eye and ENT Hospital of Fudan University, 
      Shanghai 200031, P.R. China.
FAU - Lao, Zheng
AU  - Lao Z
AD  - Radiotherapy Division, Department of Oral and Maxillofacial-Head and Neck 
      Oncology, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University 
      School of Medicine, Shanghai 200011, P.R. China.
FAU - Zhang, Haiyan
AU  - Zhang H
AD  - Department of Radiation Oncology, Eye and ENT Hospital of Fudan University, 
      Shanghai 200031, P.R. China.
FAU - Wang, Jie
AU  - Wang J
AD  - Department of Radiation Oncology, Eye and ENT Hospital of Fudan University, 
      Shanghai 200031, P.R. China.
FAU - Wang, Shengzi
AU  - Wang S
AD  - Department of Radiation Oncology, Eye and ENT Hospital of Fudan University, 
      Shanghai 200031, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
DEP - 20210715
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
RIN - Exp Ther Med. 2024 Feb 05;27(4):129. PMID: 38414792
PMC - PMC8311254
OTO - NOTNLM
OT  - circ_0001883
OT  - epithelial-mesenchymal transition
OT  - invasion
OT  - laryngeal squamous cell carcinoma
OT  - microRNA-125b-5p
OT  - migration
COIS- The authors declare that they have no competing interests.
EDAT- 2021/08/05 06:00
MHDA- 2021/08/05 06:01
PMCR- 2021/07/15
CRDT- 2021/08/04 07:06
PHST- 2020/10/16 00:00 [received]
PHST- 2021/06/09 00:00 [accepted]
PHST- 2021/08/04 07:06 [entrez]
PHST- 2021/08/05 06:00 [pubmed]
PHST- 2021/08/05 06:01 [medline]
PHST- 2021/07/15 00:00 [pmc-release]
AID - ETM-0-0-10440 [pii]
AID - 10.3892/etm.2021.10440 [doi]
PST - ppublish
SO  - Exp Ther Med. 2021 Sep;22(3):1007. doi: 10.3892/etm.2021.10440. Epub 2021 Jul 15.