PMID- 34348542
OWN - NLM
STAT- MEDLINE
DCOM- 20220202
LR  - 20220202
IS  - 1744-8387 (Electronic)
IS  - 1478-9450 (Linking)
VI  - 18
IP  - 7
DP  - 2021 Jul
TI  - Chondrocyte protein co-synthesis network analysis links ECM mechanosensing to 
      metabolic adaptation in osteoarthritis.
PG  - 623-635
LID - 10.1080/14789450.2021.1962299 [doi]
AB  - BACKGROUND: Knee osteoarthritis (OA) is one of the most common structural OA 
      disorders globally. Incomplete understanding of the fundamental biological 
      aspects of osteoarthritis underlies the current lack of effective treatment or 
      disease modifying drugs. RESEARCH DESIGN AND METHODS: We implemented a systems 
      approach by making use of the statistical network concepts in Weighted Gene 
      Co-expression Analysis to reconstruct the organization of the core proteome 
      network in chondrocytes obtained from OA patients and healthy individuals. 
      Protein modules reflect groups of tightly co-ordinated changes in protein 
      abundance across healthy and OA chondrocytes. RESULTS: The unbiased systems 
      analysis identified extracellular matrix (ECM) mechanosensing and glycolysis as 
      two modules that are most highly correlated with OmicronAlpha. The ECM module was enriched 
      in the OA genetic risk factors tenascin-C (TNC) and collagen 11A1 (COL11A1), as 
      well as in cartilage oligomeric matrix protein (COMP), a biomarker associated 
      with cartilage integrity. Mapping proteins that are unique to OA or healthy 
      chondrocytes onto the core interactome, which connects microenvironment sensing 
      and regulation of glycolysis, identified differences in metabolic and 
      anti-inflammatory adaptation. CONCLUSION: The interconnection between cartilage 
      ECM remodeling and metabolism is indicative of the dynamic chondrocyte states and 
      their significance in osteoarthritis.
FAU - Destouni, Aspasia
AU  - Destouni A
AD  - Laboratory of Cytogenetics and Molecular Genetics, Faculty of Medicine, 
      University of Thessaly, Larissa, Greece.
FAU - Tsolis, Konstantinos C
AU  - Tsolis KC
AD  - KULeuven, Department of Microbiology, Immunology and Transplantation, Rega 
      Institute for Medical Research, Laboratory of Molecular Bacteriology, Leuven, 
      Belgium.
FAU - Economou, Anastassios
AU  - Economou A
AD  - KULeuven, Department of Microbiology, Immunology and Transplantation, Rega 
      Institute for Medical Research, Laboratory of Molecular Bacteriology, Leuven, 
      Belgium.
FAU - Papathanasiou, Ioanna
AU  - Papathanasiou I
AD  - Laboratory of Cytogenetics and Molecular Genetics, Faculty of Medicine, 
      University of Thessaly, Larissa, Greece.
AD  - Department of Biology, Faculty of Medicine, University of Thessaly, Larissa, 
      Greece.
FAU - Balis, Charalampos
AU  - Balis C
AD  - Laboratory of Cytogenetics and Molecular Genetics, Faculty of Medicine, 
      University of Thessaly, Larissa, Greece.
FAU - Mourmoura, Evanthia
AU  - Mourmoura E
AD  - Laboratory of Cytogenetics and Molecular Genetics, Faculty of Medicine, 
      University of Thessaly, Larissa, Greece.
FAU - Tsezou, Aspasia
AU  - Tsezou A
AD  - Laboratory of Cytogenetics and Molecular Genetics, Faculty of Medicine, 
      University of Thessaly, Larissa, Greece.
AD  - Department of Biology, Faculty of Medicine, University of Thessaly, Larissa, 
      Greece.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210811
PL  - England
TA  - Expert Rev Proteomics
JT  - Expert review of proteomics
JID - 101223548
SB  - IM
MH  - Cells, Cultured
MH  - *Chondrocytes
MH  - Extracellular Matrix
MH  - Humans
MH  - *Osteoarthritis
OTO - NOTNLM
OT  - Chondrocyte proteome
OT  - mechanosensing
OT  - mechanotransduction
OT  - microenvironment
OT  - osteoarthritis
OT  - protein co-synthesis network analysis
EDAT- 2021/08/06 06:00
MHDA- 2022/02/03 06:00
CRDT- 2021/08/05 05:27
PHST- 2021/08/06 06:00 [pubmed]
PHST- 2022/02/03 06:00 [medline]
PHST- 2021/08/05 05:27 [entrez]
AID - 10.1080/14789450.2021.1962299 [doi]
PST - ppublish
SO  - Expert Rev Proteomics. 2021 Jul;18(7):623-635. doi: 
      10.1080/14789450.2021.1962299. Epub 2021 Aug 11.