PMID- 34349437
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220425
IS  - 0973-029X (Print)
IS  - 1998-393X (Electronic)
IS  - 0973-029X (Linking)
VI  - 25
IP  - 1
DP  - 2021 Jan-Apr
TI  - Comparative cytopathologic evaluation using acridine orange with Papanicolaou 
      stain in psychoactive substances abusers with potentially malignant and malignant 
      disorders.
PG  - 198
LID - 10.4103/jomfp.JOMFP_275_20 [doi]
AB  - INTRODUCTION: Oral cancer is a major health problem and its early detection is 
      advantageous for therapeutic purposes. According to available evidence, the risks 
      of oral malignancies increase with the usage of tobacco and other psychoactive 
      substances (PSs). The present study showed expression pattern of nuclear and 
      cytoplasmic changes from normal individuals without habit to oral potentially 
      malignant disorders (OPMD) and oral squamous cell carcinoma (OSCC) in PS abusers 
      with the help of fluorescence acridine orange (AO) stain and Papanicolaou (PAP) 
      stain. AIM AND OBJECTIVES: This study aimed to investigate and compare diagnostic 
      efficacy of fluorescence microscopic evaluation of AO stain in cytological smears 
      with PAP staining under light microscopy in PS abusers having oral potentially 
      malignant and malignant lesions. MATERIALS AND METHODS: Oral smears from 120 
      individuals among which 40 from potentially malignant disorders, 40 from oral 
      malignancy and 40 normal buccal mucosa smears were prepared. One set of smears 
      was stained by AO staining and the other by PAP staining and examined under 
      fluorescence and light microscope, respectively. The results of both the 
      stainings were evaluated by grading cytology smears in class-I to class-V 
      cytology. RESULTS: The AO fluorescence stain reliably demonstrated malignant 
      cells based on the differential fluorescence. The efficacy of AO fluorescence 
      stain was higher than PAP stain in screening of oral lesions suspicious of 
      malignancy. The sensitivity of PAP staining and AO staining is 57.50% and 61.25%, 
      respectively. CONCLUSION: As compared to PAP staining method, fluorescent AO 
      method is more effective in screening of OPMD and OSCC in PS abusers.
CI  - Copyright: (c) 2021 Journal of Oral and Maxillofacial Pathology.
FAU - Patel, Falguni H
AU  - Patel FH
AD  - Department of Oral Pathology, K. M. Shah Dental College and Hospital, Sumandeep 
      Vidyapeeth University, Vadodara, Gujarat, India.
FAU - Bhavasar, Rashmi
AU  - Bhavasar R
AD  - Department of Oral Pathology, K. M. Shah Dental College and Hospital, Sumandeep 
      Vidyapeeth University, Vadodara, Gujarat, India.
FAU - Shah, Shreyas
AU  - Shah S
AD  - Department of Oral Pathology, K. M. Shah Dental College and Hospital, Sumandeep 
      Vidyapeeth University, Vadodara, Gujarat, India.
FAU - Shah, Vandana
AU  - Shah V
AD  - Department of Oral Pathology, K. M. Shah Dental College and Hospital, Sumandeep 
      Vidyapeeth University, Vadodara, Gujarat, India.
FAU - Patel, Pratik D
AU  - Patel PD
AD  - Department of Factory Medical, Medical Officer, Alembic Pharma, Vadodara, 
      Gujarat, India.
LA  - eng
PT  - Journal Article
DEP - 20210514
PL  - India
TA  - J Oral Maxillofac Pathol
JT  - Journal of oral and maxillofacial pathology : JOMFP
JID - 101227995
PMC - PMC8272495
OTO - NOTNLM
OT  - Acridine orange stain
OT  - Papanicolaou stain
OT  - oral potentially malignant disorders
OT  - oral squamous cell carcinoma
COIS- There are no conflicts of interest.
EDAT- 2021/08/06 06:00
MHDA- 2021/08/06 06:01
PMCR- 2021/01/01
CRDT- 2021/08/05 06:21
PHST- 2020/07/07 00:00 [received]
PHST- 2021/01/10 00:00 [revised]
PHST- 2021/01/15 00:00 [accepted]
PHST- 2021/08/05 06:21 [entrez]
PHST- 2021/08/06 06:00 [pubmed]
PHST- 2021/08/06 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - JOMFP-25-198a [pii]
AID - 10.4103/jomfp.JOMFP_275_20 [doi]
PST - ppublish
SO  - J Oral Maxillofac Pathol. 2021 Jan-Apr;25(1):198. doi: 
      10.4103/jomfp.JOMFP_275_20. Epub 2021 May 14.