PMID- 34349452
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210806
IS  - 0973-6247 (Print)
IS  - 1998-3565 (Electronic)
IS  - 0973-6247 (Linking)
VI  - 15
IP  - 1
DP  - 2021 Jan-Jun
TI  - Prevalance of Anti-HLA antibodies in parous female blood donors: A pilot study 
      from tertiary care hospital of North India.
PG  - 16-20
LID - 10.4103/ajts.AJTS_30_19 [doi]
AB  - BACKGROUND: Various studies have implicated that plasma causing 
      transfusion-related acute lung injury is from alloimmunized females. The 
      frequency of sensitization to human leukocyte antigen (HLA) was found to 
      correlate with their parity score. No literature on the prevalence of anti-HLA 
      antibodies in Indian blood donors is available to date. Hence, this pilot study 
      was done to know the frequency of HLA alloimmunization in Indian blood donors. 
      MATERIALS AND METHODS: A total of 192 consenting voluntary blood donors from 
      blood donation camps were enrolled in the study. Test group: Parous female donors 
      (n = 96) and control group: Nulliparous female donors (n = 48) and male donors (n 
      = 48). HLA alloimmunization was tested on the Luminex platform by screening assay 
      to detect IgG antibodies to HLA Class I and II molecules of human origin. A mean 
      fluoresence index of more than 2000 was considered as a positive reaction, 
      considering the high sensitivity of Luminex assay. RESULTS: Sixty-three out of 
      192 donors (32.8%) tested positive for anti-HLA antibodies, out of which 23 
      donors were in the control group (23.9%), and 40 donors were in the test group 
      (41.7%); P = 0.002. On gender-based comparison, 9 out of 48 male donors (18.7%), 
      as compared to 54 out of 144 female donors (37.5%), tested positive for HLA 
      antibodies (P = 0.02). Based on an increase in parity score, the frequency of HLA 
      alloimmunization was found to be significantly correlated (P = 0.002). A decrease 
      in the trend of HLA alloimmunization was observed as the duration from the last 
      pregnancy increased. A higher frequency of HLA alloimmunization was observed in 
      female donors with a history of transfusion and bad obstetric history. 
      CONCLUSION: The present study substantiates that plasma from parous female donors 
      has a higher chance of containing anti-HLA antibodies as compared to nulliparous 
      female and male donors.
CI  - Copyright: (c) 2021 Asian Journal of Transfusion Science.
FAU - Priyadarsini, Arcot Jayachandran
AU  - Priyadarsini AJ
AD  - Department of Transfusion Medicine, AIIMS, New Delhi, India.
FAU - Dhawan, Hari Krishan
AU  - Dhawan HK
AD  - Department of Transfusion Medicine, PGIMER, Chandigarh, India.
FAU - Sharma, Ratti Ram
AU  - Sharma RR
AD  - Department of Transfusion Medicine, PGIMER, Chandigarh, India.
FAU - Saikia, Biman
AU  - Saikia B
AD  - Department of Immunopathology, PGIMER, Chandigarh, India.
FAU - Minz, Ranjana W
AU  - Minz RW
AD  - Department of Immunopathology, PGIMER, Chandigarh, India.
LA  - eng
PT  - Journal Article
DEP - 20210612
PL  - India
TA  - Asian J Transfus Sci
JT  - Asian journal of transfusion science
JID - 101306858
PMC - PMC8294446
OTO - NOTNLM
OT  - Anti-HLA antibodies
OT  - mean fluoresence index
OT  - parous female blood donors
OT  - transfusion-related acute lung injury
COIS- There are no conflicts of interest.
EDAT- 2021/08/06 06:00
MHDA- 2021/08/06 06:01
PMCR- 2021/01/01
CRDT- 2021/08/05 06:21
PHST- 2019/03/07 00:00 [received]
PHST- 2019/10/28 00:00 [revised]
PHST- 2020/08/30 00:00 [accepted]
PHST- 2021/08/05 06:21 [entrez]
PHST- 2021/08/06 06:00 [pubmed]
PHST- 2021/08/06 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - AJTS-15-16 [pii]
AID - 10.4103/ajts.AJTS_30_19 [doi]
PST - ppublish
SO  - Asian J Transfus Sci. 2021 Jan-Jun;15(1):16-20. doi: 10.4103/ajts.AJTS_30_19. 
      Epub 2021 Jun 12.