PMID- 34350121
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240403
IS  - 2234-943X (Print)
IS  - 2234-943X (Electronic)
IS  - 2234-943X (Linking)
VI  - 11
DP  - 2021
TI  - Differential Expression Profile of lncRNA in Glioma Cells and the Effect of 
      lncRNA NKX3-1 on Glioma Cells Through Fem1b/SPDEF Pathway.
PG  - 706863
LID - 10.3389/fonc.2021.706863 [doi]
LID - 706863
AB  - OBJECTIVE: To investigate the differential expression of lncRNA in glioma cells, 
      as well as the effect of lncRNA NKX3-1 on glioma cells. METHODS: Glioma-related 
      data were first downloaded from the TCGA database and analyzed using 
      bioinformatics, after which the lncRNA NKX3-1 was chosen for further experiments. 
      The expression of the lncRNA NKX3-1 in glioma tumor samples was detected using 
      qRT-PCR. The subcellular localization of lncRNA NKX3-1 was determined using 
      fluorescence in situ hybridization (FISH). CCK-8, flow cytometry, cell scratch, 
      and transwell assays were used to detect cell proliferation, apoptosis, and 
      invasion. The downstream pathway of lncRNA NKX3-1 was investigated using 
      luciferase assays and detected using western blot, transwell, and cell scratch 
      assays. RESULTS: The differential expression profile of lncRNA in glioma was 
      obtained. NKX3-1 lncRNA was found to be significantly increased in glioma tumor 
      tissues. LncRNA NKX3-1 was found in the nucleus. Proliferation, invasion, and 
      migration of glioma cells were significantly increased (P <0.05) in the lncRNA 
      NKX3-1 overexpression group, while apoptosis ability was significantly decreased 
      (P <0.05). Tumor volume and weight were significantly increased in the lncRNA 
      NKX3-1 overexpression group in nude mice (P <0.05). LncRNA NKX3-1 significantly 
      increased the luciferase activity of Fem1b 3'-UTR-WT reporter genes (P <0.05) as 
      well as the levels of SPDEF protein (P <0.05). The protein level of FEM1B was 
      significantly reduced. Cell invasion and migration were significantly increased 
      (P <0.05) in the lncRNA NKX3-1 overexpression group plus SPDEF group. CONCLUSION: 
      We investigated the differential expression profile of lncRNAs in glioma and 
      discovered that the lncRNA NKX3-1 plays an important role in cancer promotion via 
      the Fem1b/SPDEF pathway.
CI  - Copyright (c) 2021 Cai, Wang, Cui, Tan and Jiang.
FAU - Cai, Yang
AU  - Cai Y
AD  - Department of Neurosurgery, The Second Xiangya Hospital, Central South 
      University, Changsha, China.
FAU - Wang, Ming
AU  - Wang M
AD  - Department of Neurosurgery, The Second Xiangya Hospital, Central South 
      University, Changsha, China.
FAU - Cui, Yan
AU  - Cui Y
AD  - Department of Neurosurgery, The Second Xiangya Hospital, Central South 
      University, Changsha, China.
FAU - Tan, Zhigang
AU  - Tan Z
AD  - Department of Neurosurgery, The Second Xiangya Hospital, Central South 
      University, Changsha, China.
FAU - Jiang, Yugang
AU  - Jiang Y
AD  - Department of Neurosurgery, The Second Xiangya Hospital, Central South 
      University, Changsha, China.
LA  - eng
PT  - Journal Article
DEP - 20210719
PL  - Switzerland
TA  - Front Oncol
JT  - Frontiers in oncology
JID - 101568867
PMC - PMC8328487
OTO - NOTNLM
OT  - FEM1B
OT  - NKX3-1
OT  - SPDEF
OT  - glioma
OT  - lncRNA
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/08/06 06:00
MHDA- 2021/08/06 06:01
PMCR- 2021/01/01
CRDT- 2021/08/05 06:29
PHST- 2021/05/08 00:00 [received]
PHST- 2021/06/28 00:00 [accepted]
PHST- 2021/08/05 06:29 [entrez]
PHST- 2021/08/06 06:00 [pubmed]
PHST- 2021/08/06 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fonc.2021.706863 [doi]
PST - epublish
SO  - Front Oncol. 2021 Jul 19;11:706863. doi: 10.3389/fonc.2021.706863. eCollection 
      2021.