PMID- 34350523
OWN - NLM
STAT- MEDLINE
DCOM- 20220214
LR  - 20220224
IS  - 1435-4373 (Electronic)
IS  - 0934-9723 (Print)
IS  - 0934-9723 (Linking)
VI  - 41
IP  - 3
DP  - 2022 Mar
TI  - Rapid molecular detection of pathogenic microorganisms and antimicrobial 
      resistance markers in blood cultures: evaluation and utility of the 
      next-generation FilmArray Blood Culture Identification 2 panel.
PG  - 363-371
LID - 10.1007/s10096-021-04314-2 [doi]
AB  - Rapid detection of pathogens causing bloodstream infections (BSI) directly from 
      positive blood cultures is of highest importance in order to enable an adequate 
      and timely antimicrobial therapy. In this study, the utility and performance of a 
      recently launched next-generation fully automated test system, the Biofire 
      FilmArray(R) Blood Culture Identification 2 (BCID2) panel, was evaluated using a 
      set of 103 well-characterized microbial isolates including 29 antimicrobial 
      resistance genes and 80 signal-positive and 23 signal-negative clinical blood 
      culture samples. The results were compared to culture-based reference methods, 
      MALDI-TOF, and/or 16S rDNA sequencing. Of the clinical blood culture samples, 68 
      were monomicrobial (85.0%) and 12 polymicrobial (15.0%). Six samples contained 
      ESBL (bla(CTX-M)), two MRSA (mecA), and three MRSE (mecA) isolates. In overall, 
      the FilmArray BCID2 panel detected well on-panel targets and resistance markers 
      from mono- and polymicrobial samples. However, one Klebsiella aerogenes and one 
      Bacteroides ovatus were undetected, and the assay falsely reported one Shigella 
      flexneri as Escherichia coli. Hence, the sensitivity and specificity for 
      detecting microbial species were 98.8% (95%CI, 95.8-99.9%) and 99.9% (95%CI, 
      99.8-99.9%), respectively. The sensitivity and specificity for detecting of 
      resistance gene markers were 100%. The results were available within 70 min from 
      signal-positive blood cultures with minimal hands-on time. In conclusion, the 
      BCID2 test allows reliable and simplified detection of a vast variety of 
      clinically relevant microbes causing BSI and the most common antimicrobial 
      resistance markers present among these isolates.
CI  - (c) 2021. The Author(s).
FAU - Holma, Tanja
AU  - Holma T
AD  - HUS Diagnostic Center, HUSLAB, Department of Clinical Microbiology, University of 
      Helsinki and Helsinki University Hospital, Helsinki, Finland. tanja.holma@hus.fi.
FAU - Torvikoski, Jukka
AU  - Torvikoski J
AD  - HUS Diagnostic Center, HUSLAB, Department of Clinical Microbiology, University of 
      Helsinki and Helsinki University Hospital, Helsinki, Finland.
FAU - Friberg, Nathalie
AU  - Friberg N
AD  - HUS Diagnostic Center, HUSLAB, Department of Clinical Microbiology, University of 
      Helsinki and Helsinki University Hospital, Helsinki, Finland.
FAU - Nevalainen, Annika
AU  - Nevalainen A
AD  - HUS Diagnostic Center, HUSLAB, Department of Clinical Microbiology, University of 
      Helsinki and Helsinki University Hospital, Helsinki, Finland.
FAU - Tarkka, Eveliina
AU  - Tarkka E
AD  - HUS Diagnostic Center, HUSLAB, Department of Clinical Microbiology, University of 
      Helsinki and Helsinki University Hospital, Helsinki, Finland.
FAU - Antikainen, Jenni
AU  - Antikainen J
AD  - HUS Diagnostic Center, HUSLAB, Department of Clinical Microbiology, University of 
      Helsinki and Helsinki University Hospital, Helsinki, Finland.
FAU - Martelin, Jari J
AU  - Martelin JJ
AD  - HUS Diagnostic Center, HUSLAB, Department of Clinical Microbiology, University of 
      Helsinki and Helsinki University Hospital, Helsinki, Finland.
LA  - eng
PT  - Journal Article
DEP - 20210805
PL  - Germany
TA  - Eur J Clin Microbiol Infect Dis
JT  - European journal of clinical microbiology & infectious diseases : official 
      publication of the European Society of Clinical Microbiology
JID - 8804297
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Anti-Infective Agents)
SB  - IM
EIN - Eur J Clin Microbiol Infect Dis. 2022 Feb 8;:. PMID: 35133542
MH  - Anti-Bacterial Agents/pharmacology
MH  - *Anti-Infective Agents
MH  - *Bacteremia/diagnosis
MH  - Bacteria/genetics
MH  - Blood Culture
MH  - Drug Resistance, Bacterial
MH  - Humans
PMC - PMC8831274
OTO - NOTNLM
OT  - Antimicrobial resistance
OT  - BSI
OT  - Bacteremia
OT  - Blood culture
OT  - Molecular detection methods
OT  - Rapid diagnostics
COIS- The authors declare no competing interests.
EDAT- 2021/08/06 06:00
MHDA- 2022/02/15 06:00
PMCR- 2021/08/05
CRDT- 2021/08/05 06:38
PHST- 2021/03/09 00:00 [received]
PHST- 2021/07/09 00:00 [accepted]
PHST- 2021/08/06 06:00 [pubmed]
PHST- 2022/02/15 06:00 [medline]
PHST- 2021/08/05 06:38 [entrez]
PHST- 2021/08/05 00:00 [pmc-release]
AID - 10.1007/s10096-021-04314-2 [pii]
AID - 4314 [pii]
AID - 10.1007/s10096-021-04314-2 [doi]
PST - ppublish
SO  - Eur J Clin Microbiol Infect Dis. 2022 Mar;41(3):363-371. doi: 
      10.1007/s10096-021-04314-2. Epub 2021 Aug 5.