PMID- 34354522
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210807
IS  - 1880-7291 (Electronic)
IS  - 1344-7882 (Print)
IS  - 1344-7882 (Linking)
VI  - 66
IP  - 2
DP  - 2019
TI  - Construction of Cellulose Binding Domain Fusion FMN-Dependent NADH-Azoreductase 
      and Glucose 1-Dehydrogenase for the Development of Flow Injection Analysis with 
      Fusion Enzymes Immobilized on Cellulose.
PG  - 65-72
LID - 10.5458/jag.jag.JAG-2018_0011 [doi]
AB  - The cellulose binding domain (CBD) of cellulosome-integrating protein A from 
      Clostridium thermocellum NBRC 103400 was genetically fused to FMN-dependent 
      NADH-azoreductase (AZR) and glucose 1-dehydrogenase (GDH) from Bacillus subtilis. 
      The fusion enzymes, AZR-CBD and CBD-GDH, were expressed in Escherichia coli 
      Rosetta-gami B (DE3). The enzymes were purified from cell-free extracts, and the 
      specific activity of AZR-CBD was 15.1 U/mg and that of CBD-GDH was 22.6 U/mg. 
      AZR-CBD and CBD-GDH bound strongly to 0.5 % swollen cellulose at approximately 95 
      and 98 % of the initial protein amounts, respectively. After immobilization onto 
      the swollen cellulose, AZR-CBD and CBD-GDH retained their catalytic activity. 
      Both enzymes bound weakly to 0.5 % microcrystalline cellulose, but the addition 
      of a high concentration of microcrystalline cellulose (10 %) improved the binding 
      rate of both enzymes. A reactor for flow injection analysis was filled with 
      microcrystalline cellulose-immobilized AZR-CBD and CBD-GDH. This flow injection 
      analysis system was successfully applied for the determination of glucose, and a 
      linear calibration curve was observed in the range of approximately 0.16-2.5 mM 
      glucose, with a correlation coefficient, r, of 0.998.
CI  - 2019 by The Japanese Society of Applied Glycoscience.
FAU - Yano, Shigekazu
AU  - Yano S
AD  - 1 Department of Biochemical Engineering, Graduate School of Sciences and 
      Engineering, Yamagata University.
FAU - Hori, Yukari
AU  - Hori Y
AD  - 1 Department of Biochemical Engineering, Graduate School of Sciences and 
      Engineering, Yamagata University.
FAU - Kijima, Tatsuro
AU  - Kijima T
AD  - 1 Department of Biochemical Engineering, Graduate School of Sciences and 
      Engineering, Yamagata University.
FAU - Konno, Hiroyuki
AU  - Konno H
AD  - 1 Department of Biochemical Engineering, Graduate School of Sciences and 
      Engineering, Yamagata University.
FAU - Suyotha, Wasana
AU  - Suyotha W
AD  - 2 Department of Industrial Biotechnology, Faculty of Agro-industry.
FAU - Takagi, Kazuyoshi
AU  - Takagi K
AD  - 3 Department of Applied Chemistry, Faculty of Life Sciences, Ritsumeikan 
      University.
FAU - Wakayama, Mamoru
AU  - Wakayama M
AD  - 4 Department of Biotechnology, Faculty of Life Sciences, Ritsumeikan University.
LA  - eng
PT  - Journal Article
DEP - 20190521
PL  - Japan
TA  - J Appl Glycosci (1999)
JT  - Journal of applied glycoscience
JID - 101167786
PMC - PMC8056932
OTO - NOTNLM
OT  - FMN-dependent NADH-azoreductase
OT  - cellulose binding domain
OT  - enzyme immobilization
OT  - glucose dehydrogenase
COIS- The authors declare no conflict of interests.
EDAT- 2019/05/21 00:00
MHDA- 2019/05/21 00:01
PMCR- 2019/05/21
CRDT- 2021/08/06 06:48
PHST- 2018/11/13 00:00 [received]
PHST- 2019/01/10 00:00 [accepted]
PHST- 2021/08/06 06:48 [entrez]
PHST- 2019/05/21 00:00 [pubmed]
PHST- 2019/05/21 00:01 [medline]
PHST- 2019/05/21 00:00 [pmc-release]
AID - 10.5458/jag.jag.JAG-2018_0011 [doi]
PST - epublish
SO  - J Appl Glycosci (1999). 2019 May 21;66(2):65-72. doi: 
      10.5458/jag.jag.JAG-2018_0011. eCollection 2019.