PMID- 34357046
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210809
IS  - 2075-1729 (Print)
IS  - 2075-1729 (Electronic)
IS  - 2075-1729 (Linking)
VI  - 11
IP  - 7
DP  - 2021 Jul 10
TI  - Do Seminal Isoprostanes Have a Role in Assisted Reproduction Outcome?
LID - 10.3390/life11070675 [doi]
LID - 675
AB  - F(2)-isoprostanes (F(2)-IsoPs), stereoisomers of prostaglandin F2alpha generated by 
      the free radical-induced oxidation of arachidonic acid, have been associated with 
      different male infertility conditions. This study aimed to evaluate the role of 
      seminal isoprostane levels and sperm characteristics in the reproductive outcome 
      and embryo quality of 49 infertile couples. Semen analysis was performed 
      following WHO guidelines. Sperm chromatin maturity was detected using an aniline 
      blue (AB) assay, and DNA integrity was assessed using the acridine orange (AO) 
      test. Seminal F(2)-IsoP levels were quantified by gas chromatography/negative ion 
      chemical ionization tandem mass spectrometry (GC/NICI-MS/MS) analysis. 
      Correlations among variables and their impact on in vitro fertilization (IVF) and 
      intracytoplasmic sperm injection (ICSI) outcome were investigated. F(2)-IsoP 
      levels are positively correlated with double-stranded DNA sperm (p < 0.001) and 
      negatively correlated with mature sperm chromatin (p < 0.001). Patients with 
      positive outcomes had an increased percentage of sperm with double-stranded DNA, 
      as did patients producing high-quality embryo, who showed higher F(2)-IsoP levels 
      compared to those detected in the low-quality embryo group. An intriguing 
      relationship between a mild increase in F(2)-IsoP levels, DNA integrity, and 
      embryo quality seems to indicate that the non-enzymatic oxidation of arachidonic 
      acid can be also a marker of metabolic activity in human semen.
FAU - Collodel, Giulia
AU  - Collodel G
AUID- ORCID: 0000-0003-1587-0159
AD  - Department of Molecular and Developmental Medicine, University of Siena, 53100 
      Siena, Italy.
FAU - Noto, Daria
AU  - Noto D
AUID- ORCID: 0000-0002-1571-9056
AD  - Department of Molecular and Developmental Medicine, University of Siena, 53100 
      Siena, Italy.
FAU - Signorini, Cinzia
AU  - Signorini C
AUID- ORCID: 0000-0003-0006-9414
AD  - Department of Molecular and Developmental Medicine, University of Siena, 53100 
      Siena, Italy.
FAU - Gambera, Laura
AU  - Gambera L
AD  - Fertility Center, AGI Medica, 53100 Siena, Italy.
FAU - Stendardi, Anita
AU  - Stendardi A
AD  - Fertility Center, AGI Medica, 53100 Siena, Italy.
FAU - Mahmutbegovic, Amra
AU  - Mahmutbegovic A
AD  - Department of Molecular and Developmental Medicine, University of Siena, 53100 
      Siena, Italy.
FAU - Micheli, Lucia
AU  - Micheli L
AUID- ORCID: 0000-0002-0306-9308
AD  - Department of Medicine, Surgery and Neurosciences, University of Siena, 53100 
      Siena, Italy.
FAU - Menchiari, Andrea
AU  - Menchiari A
AUID- ORCID: 0000-0002-8194-0613
AD  - Department of Business and Law, University of Siena, 53100 Siena, Italy.
FAU - Moretti, Elena
AU  - Moretti E
AUID- ORCID: 0000-0002-9467-0501
AD  - Department of Molecular and Developmental Medicine, University of Siena, 53100 
      Siena, Italy.
LA  - eng
PT  - Journal Article
DEP - 20210710
PL  - Switzerland
TA  - Life (Basel)
JT  - Life (Basel, Switzerland)
JID - 101580444
PMC - PMC8303377
OTO - NOTNLM
OT  - embryo quality
OT  - in vitro fertilization outcome
OT  - male infertility
OT  - seminal F2-isoprostanes
OT  - sperm chromatin
COIS- The authors declare no conflict of interest.
EDAT- 2021/08/07 06:00
MHDA- 2021/08/07 06:01
PMCR- 2021/07/10
CRDT- 2021/08/06 12:22
PHST- 2021/06/16 00:00 [received]
PHST- 2021/07/01 00:00 [revised]
PHST- 2021/07/08 00:00 [accepted]
PHST- 2021/08/06 12:22 [entrez]
PHST- 2021/08/07 06:00 [pubmed]
PHST- 2021/08/07 06:01 [medline]
PHST- 2021/07/10 00:00 [pmc-release]
AID - life11070675 [pii]
AID - life-11-00675 [pii]
AID - 10.3390/life11070675 [doi]
PST - epublish
SO  - Life (Basel). 2021 Jul 10;11(7):675. doi: 10.3390/life11070675.