PMID- 34359112
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240402
IS  - 2076-2615 (Print)
IS  - 2076-2615 (Electronic)
IS  - 2076-2615 (Linking)
VI  - 11
IP  - 7
DP  - 2021 Jul 2
TI  - Conservation of Imprinting and Methylation of MKRN3, MAGEL2 and NDN Genes in 
      Cattle.
LID - 10.3390/ani11071985 [doi]
LID - 1985
AB  - Genomic imprinting is the epigenetic mechanism of transcriptional regulation that 
      involves differential DNA methylation modification. Comparative analysis of 
      imprinted genes between species can help us to investigate the biological 
      significance and regulatory mechanisms of genomic imprinting. MKRN3, MAGEL2 and 
      NDN are three maternally imprinted genes identified in the human PWS/AS imprinted 
      locus. This study aimed to assess the allelic expression of MKRN3, MAGEL2 and NDN 
      and to examine the differentially methylated regions (DMRs) of bovine PWS/AS 
      imprinted domains. An expressed single-nucleotide polymorphism (SNP)-based 
      approach was used to investigate the allelic expression of MKRN3, MAGEL2 and NDN 
      genes in bovine adult tissues and placenta. Consistent with the expression in 
      humans and mice, we found that the MKRN3, MAGEL2 and NDN genes exhibit 
      monoallelic expression in bovine somatic tissues and the paternal allele 
      expressed in the bovine placenta. Three DMRs, PWS-IC, MKRN3 and NDN DMR, were 
      identified in the bovine PWS/AS imprinted region by analysis of the DNA 
      methylation status in bovine tissues using the bisulfite sequencing method and 
      were located in the promoter and exon 1 of the SNRPN gene, NDN promoter and 5' 
      untranslated region (5'UTR) of MKRN3 gene, respectively. The PWS-IC DMR is a 
      primary DMR inherited from the male or female gamete, but NDN and MKRN3 DMR are 
      secondary DMRs that occurred after fertilization by examining the methylation 
      status in gametes.
FAU - Li, Junliang
AU  - Li J
AD  - College of Life Science, Agricultural University of Hebei, Baoding 071000, China.
FAU - Chen, Weina
AU  - Chen W
AD  - Department of Traditional Chinese Medicine, Hebei University, Baoding 071000, 
      China.
FAU - Li, Dongjie
AU  - Li D
AD  - College of Bioscience and Bioengineering, Hebei University of Science and 
      Technology, Shijiazhuang 050081, China.
FAU - Gu, Shukai
AU  - Gu S
AD  - College of Life Science, Agricultural University of Hebei, Baoding 071000, China.
FAU - Liu, Xiaoqian
AU  - Liu X
AD  - College of Life Science, Agricultural University of Hebei, Baoding 071000, China.
FAU - Dong, Yanqiu
AU  - Dong Y
AD  - College of Life Science, Agricultural University of Hebei, Baoding 071000, China.
FAU - Jin, Lanjie
AU  - Jin L
AD  - College of Life Science, Agricultural University of Hebei, Baoding 071000, China.
FAU - Zhang, Cui
AU  - Zhang C
AD  - College of Life Science, Agricultural University of Hebei, Baoding 071000, China.
FAU - Li, Shijie
AU  - Li S
AD  - College of Life Science, Agricultural University of Hebei, Baoding 071000, China.
LA  - eng
GR  - 31372312/National Natural Science Foundation of China/
GR  - C2020204004/Hebei Province Natural Science Foundation of China/
PT  - Journal Article
DEP - 20210702
PL  - Switzerland
TA  - Animals (Basel)
JT  - Animals : an open access journal from MDPI
JID - 101635614
PMC - PMC8300276
OTO - NOTNLM
OT  - DMR
OT  - DNA methylation
OT  - MAGEL2 gene
OT  - MKRN3 gene
OT  - NDN gene
OT  - cow
OT  - imprinting
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript, or in the decision to publish the results.
EDAT- 2021/08/08 06:00
MHDA- 2021/08/08 06:01
PMCR- 2021/07/02
CRDT- 2021/08/07 01:01
PHST- 2021/06/02 00:00 [received]
PHST- 2021/06/25 00:00 [revised]
PHST- 2021/06/30 00:00 [accepted]
PHST- 2021/08/07 01:01 [entrez]
PHST- 2021/08/08 06:00 [pubmed]
PHST- 2021/08/08 06:01 [medline]
PHST- 2021/07/02 00:00 [pmc-release]
AID - ani11071985 [pii]
AID - animals-11-01985 [pii]
AID - 10.3390/ani11071985 [doi]
PST - epublish
SO  - Animals (Basel). 2021 Jul 2;11(7):1985. doi: 10.3390/ani11071985.