PMID- 34359982
OWN - NLM
STAT- MEDLINE
DCOM- 20211026
LR  - 20240226
IS  - 2073-4409 (Electronic)
IS  - 2073-4409 (Linking)
VI  - 10
IP  - 7
DP  - 2021 Jul 17
TI  - Protective Response in Experimental Paracoccidioidomycosis Elicited by 
      Extracellular Vesicles Containing Antigens of Paracoccidioides brasiliensis.
LID - 10.3390/cells10071813 [doi]
LID - 1813
AB  - Paracoccidioidomycosis (PCM) is a systemic disease caused by Paracoccidioides 
      spp. PCM is endemic in Latin America and most cases are registered in Brazil. 
      This mycosis affects mainly the lungs, but can also spread to other tissues and 
      organs, including the liver. Several approaches have been investigated to improve 
      treatment effectiveness and protection against the disease. Extracellular 
      vesicles (EVs) are good antigen delivery vehicles. The present work aims to 
      investigate the use of EVs derived from Paracoccidioides brasiliensis as an 
      immunization tool in a murine model of PCM. For this, male C57BL/6 were immunized 
      with two doses of EVs plus adjuvant and then infected with P. brasiliensis. EV 
      immunization induced IgM and IgG in vivo and cytokine production by splenocytes 
      ex vivo. Further, immunization with EVs had a positive effect on mice infected 
      with P. brasiliensis, as it induced activated T lymphocytes and NKT cell 
      mobilization to the infected lungs, improved production of proinflammatory 
      cytokines and the histopathological profile, and reduced fungal burden. 
      Therefore, the present study shows a new role for P. brasiliensis EVs in the 
      presence of adjuvant as modulators of the host immune system, suggesting their 
      utility as immunizing agents.
FAU - Baltazar, Ludmila Matos
AU  - Baltazar LM
AD  - Laboratorio de Interacao Microrganismo Hospedeiro, Departamento de Microbiologia, 
      Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, 6627, 
      Antonio Carlos Ave, Belo Horizonte, Minas Gerais 31270-901, Brazil.
FAU - Ribeiro, Gabriela Fior
AU  - Ribeiro GF
AD  - Laboratorio de Interacao Microrganismo Hospedeiro, Departamento de Microbiologia, 
      Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, 6627, 
      Antonio Carlos Ave, Belo Horizonte, Minas Gerais 31270-901, Brazil.
FAU - Freitas, Gustavo J
AU  - Freitas GJ
AD  - Departamento de Microbiologia, Instituto de Ciencias Biologicas, Universidade 
      Federal de Minas Gerais, 6627, Antonio Carlos Ave, Belo Horizonte, Minas Gerais 
      31270-901, Brazil.
FAU - Queiroz-Junior, Celso Martins
AU  - Queiroz-Junior CM
AD  - Departamento de Morfologia, Instituto de Ciencias Biologicas, Universidade 
      Federal de Minas Gerais, 6627, Antonio Carlos Ave, Belo Horizonte, Minas Gerais 
      31270-901, Brazil.
FAU - Fagundes, Caio Tavares
AU  - Fagundes CT
AUID- ORCID: 0000-0002-6747-5233
AD  - Laboratorio de Interacao Microrganismo Hospedeiro, Departamento de Microbiologia, 
      Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, 6627, 
      Antonio Carlos Ave, Belo Horizonte, Minas Gerais 31270-901, Brazil.
FAU - Chaves-Olortegui, Carlos
AU  - Chaves-Olortegui C
AD  - Departamento de Bioquimica e Imunologia, Instituto de Ciencias Biologicas, 
      Universidade Federal de Minas Gerais, 6627, Antonio Carlos Ave, Belo Horizonte, 
      Minas Gerais 31270-901, Brazil.
FAU - Teixeira, Mauro Martins
AU  - Teixeira MM
AD  - Departamento de Bioquimica e Imunologia, Instituto de Ciencias Biologicas, 
      Universidade Federal de Minas Gerais, 6627, Antonio Carlos Ave, Belo Horizonte, 
      Minas Gerais 31270-901, Brazil.
FAU - Souza, Daniele G
AU  - Souza DG
AUID- ORCID: 0000-0002-7478-5934
AD  - Laboratorio de Interacao Microrganismo Hospedeiro, Departamento de Microbiologia, 
      Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, 6627, 
      Antonio Carlos Ave, Belo Horizonte, Minas Gerais 31270-901, Brazil.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210717
PL  - Switzerland
TA  - Cells
JT  - Cells
JID - 101600052
RN  - 0 (Antibodies, Fungal)
RN  - 0 (Antigens, Fungal)
RN  - 0 (Cytokines)
RN  - 0 (Protective Agents)
SB  - IM
MH  - Animals
MH  - Antibodies, Fungal/immunology
MH  - Antigens, Fungal/*immunology
MH  - Cell Movement
MH  - Cytokines/metabolism
MH  - Extracellular Vesicles/drug effects/*microbiology
MH  - Immunization
MH  - Immunologic Memory
MH  - Lung/microbiology/pathology
MH  - Lymphocyte Activation/immunology
MH  - Male
MH  - Mice, Inbred C57BL
MH  - Natural Killer T-Cells/immunology
MH  - Paracoccidioides/*immunology
MH  - Paracoccidioidomycosis/*immunology/*microbiology
MH  - Protective Agents/*pharmacology
MH  - Reference Standards
MH  - Mice
PMC - PMC8304155
OTO - NOTNLM
OT  - Paracoccidioides brasiliensis
OT  - extracellular vesicles
OT  - host response
OT  - immunization
COIS- The authors declare no conflict of interest.
EDAT- 2021/08/08 06:00
MHDA- 2021/10/27 06:00
PMCR- 2021/07/17
CRDT- 2021/08/07 01:04
PHST- 2021/06/13 00:00 [received]
PHST- 2021/07/10 00:00 [revised]
PHST- 2021/07/13 00:00 [accepted]
PHST- 2021/08/07 01:04 [entrez]
PHST- 2021/08/08 06:00 [pubmed]
PHST- 2021/10/27 06:00 [medline]
PHST- 2021/07/17 00:00 [pmc-release]
AID - cells10071813 [pii]
AID - cells-10-01813 [pii]
AID - 10.3390/cells10071813 [doi]
PST - epublish
SO  - Cells. 2021 Jul 17;10(7):1813. doi: 10.3390/cells10071813.