PMID- 34359988
OWN - NLM
STAT- MEDLINE
DCOM- 20211026
LR  - 20211026
IS  - 2073-4409 (Electronic)
IS  - 2073-4409 (Linking)
VI  - 10
IP  - 7
DP  - 2021 Jul 19
TI  - New Data on Organization and Spatial Localization of B-Chromosomes in Cell Nuclei 
      of the Yellow-Necked Mouse Apodemus flavicollis.
LID - 10.3390/cells10071819 [doi]
LID - 1819
AB  - The gene composition, function and evolution of B-chromosomes (Bs) have been 
      actively discussed in recent years. However, the additional genomic elements are 
      still enigmatic. One of Bs mysteries is their spatial organization in the 
      interphase nucleus. It is known that heterochromatic compartments are not 
      randomly localized in a nucleus. The purpose of this work was to study the 
      organization and three-dimensional spatial arrangement of Bs in the interphase 
      nucleus. Using microdissection of Bs and autosome centromeric heterochromatic 
      regions of the yellow-necked mouse (Apodemus flavicollis) we obtained DNA probes 
      for further two-dimensional (2D)- and three-dimensional (3D)- fluorescence in 
      situ hybridization (FISH) studies. Simultaneous in situ hybridization of obtained 
      here B-specific DNA probes and autosomal C-positive pericentromeric 
      region-specific probes further corroborated the previously stated hypothesis 
      about the pseudoautosomal origin of the additional chromosomes of this species. 
      Analysis of the spatial organization of the Bs demonstrated the peripheral 
      location of B-specific chromatin within the interphase nucleus and feasible 
      contact with the nuclear envelope (similarly to pericentromeric regions of 
      autosomes and sex chromosomes). It is assumed that such interaction is essential 
      for the regulation of nuclear architecture. It also points out that Bs may follow 
      the same mechanism as sex chromosomes to avoid a meiotic checkpoint.
FAU - Karamysheva, Tatyana
AU  - Karamysheva T
AUID- ORCID: 0000-0002-5140-4350
AD  - Institute of Cytology and Genetics, The Siberian Branch of the Russian Academy of 
      Sciences, 630090 Novosibirsk, Russia.
FAU - Romanenko, Svetlana
AU  - Romanenko S
AUID- ORCID: 0000-0002-0951-5209
AD  - Institute of Molecular and Cellular Biology, The Siberian Branch of the Russian 
      Academy of Sciences, 630090 Novosibirsk, Russia.
FAU - Makunin, Alexey
AU  - Makunin A
AUID- ORCID: 0000-0002-9555-5097
AD  - Wellcome Sanger Institute, Cambridge CB10 1SA, UK.
FAU - Rajicic, Marija
AU  - Rajicic M
AUID- ORCID: 0000-0002-2470-9888
AD  - Institute for Biological Research "Sinisa Stankovic", National Institute of 
      Republic of Serbia, 11060 Belgrade, Serbia.
FAU - Bogdanov, Alexey
AU  - Bogdanov A
AUID- ORCID: 0000-0002-2106-3989
AD  - Koltzov Institute of Developmental Biology, Russian Academy of Sciences, 119991 
      Moscow, Russia.
FAU - Trifonov, Vladimir
AU  - Trifonov V
AUID- ORCID: 0000-0003-0454-8359
AD  - Institute of Molecular and Cellular Biology, The Siberian Branch of the Russian 
      Academy of Sciences, 630090 Novosibirsk, Russia.
AD  - Department of Genetic Technologies, Novosibirsk State University, 630090 
      Novosibirsk, Russia.
FAU - Blagojevic, Jelena
AU  - Blagojevic J
AUID- ORCID: 0000-0001-7102-5510
AD  - Institute for Biological Research "Sinisa Stankovic", National Institute of 
      Republic of Serbia, 11060 Belgrade, Serbia.
FAU - Vujosevic, Mladen
AU  - Vujosevic M
AUID- ORCID: 0000-0002-5577-4455
AD  - Institute for Biological Research "Sinisa Stankovic", National Institute of 
      Republic of Serbia, 11060 Belgrade, Serbia.
FAU - Orishchenko, Konstantin
AU  - Orishchenko K
AUID- ORCID: 0000-0001-9718-9038
AD  - Institute of Cytology and Genetics, The Siberian Branch of the Russian Academy of 
      Sciences, 630090 Novosibirsk, Russia.
AD  - Department of Genetic Technologies, Novosibirsk State University, 630090 
      Novosibirsk, Russia.
FAU - Rubtsov, Nikolay
AU  - Rubtsov N
AUID- ORCID: 0000-0001-7406-8057
AD  - Institute of Cytology and Genetics, The Siberian Branch of the Russian Academy of 
      Sciences, 630090 Novosibirsk, Russia.
AD  - Department of Genetic Technologies, Novosibirsk State University, 630090 
      Novosibirsk, Russia.
LA  - eng
GR  - 19-015-00084small a, Cyrillic/Russian Foundation for Basic Research/
GR  - 0259-2021-0011/the State Budget of ICG and by the Ministry of Science and Higher 
      Education of the Russian Federation via IC&G SB RAS/
GR  - 0088-2021-0019/Government basic research program/
GR  - 2019-0546 (FSUS-2020-0040)/the Ministry of Science and Higher Education of the 
      Russian Federation via the Novosibirsk State University/
GR  - 206194/WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210719
PL  - Switzerland
TA  - Cells
JT  - Cells
JID - 101600052
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/metabolism
MH  - Cell Nucleus/*genetics
MH  - Centromere/genetics
MH  - Chromosome Painting
MH  - Chromosomes, Mammalian/*genetics
MH  - DNA/genetics
MH  - Fibroblasts/metabolism
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Metaphase/genetics
MH  - Murinae/*genetics
PMC - PMC8305704
OTO - NOTNLM
OT  - 3D genome organization
OT  - B-chromosomes (Bs)
OT  - chromosome territories (CT)
OT  - confocal microscopy
OT  - duplication clusters
OT  - microdissection
OT  - repetitive DNA
OT  - speciation
COIS- All authors have no conflict of interest to declare.
EDAT- 2021/08/08 06:00
MHDA- 2021/10/27 06:00
PMCR- 2021/07/19
CRDT- 2021/08/07 01:04
PHST- 2021/06/29 00:00 [received]
PHST- 2021/07/13 00:00 [revised]
PHST- 2021/07/14 00:00 [accepted]
PHST- 2021/08/07 01:04 [entrez]
PHST- 2021/08/08 06:00 [pubmed]
PHST- 2021/10/27 06:00 [medline]
PHST- 2021/07/19 00:00 [pmc-release]
AID - cells10071819 [pii]
AID - cells-10-01819 [pii]
AID - 10.3390/cells10071819 [doi]
PST - epublish
SO  - Cells. 2021 Jul 19;10(7):1819. doi: 10.3390/cells10071819.